TR
EN
EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES
Abstract
Vegetables are vital sources of dietary minerals, particularly for individuals following plant-based diets. However, the total mineral content does not necessarily reflect its bioaccessibility, which determines the proportion available for absorption in the gastrointestinal tract. This study evaluates the elemental composition and in-vitro mineral bioaccessibility of commonly consumed vegetables, including spinach (Spinacia oleracea), lettuce (Lactuca sativa), cucumber (Cucumis sativus), broccoli (Brassica oleracea), and parsley (Petroselinum crispum). Mineral concentrations of K, Mg, Ca, Na, Fe, Mn, and Zn were quantified using ICP-OES following microwave-assisted digestion. An in-vitro digestion model simulated gastric and intestinal conditions. The highest Fe bioaccessibility was 18.82%, while K, Mg, and Ca were most bioaccessible in cucumber (21.9%), lettuce (25.09%), and parsley (23.42%), respectively. These findings highlight the importance of considering bioaccessibility in nutritional assessments. Future studies should investigate the effects of cooking methods and soil composition on mineral bioavailability to improve dietary recommendations.
Keywords
Supporting Institution
TUBITAK
Project Number
1919B012305356
Thanks
This study was derived from the project titled “Vegan – Vejetaryen Beslenme Eğilimlerinde Sıklıkla Kullanılan Yeşil Sebzelerin In-Vitro Mineral Biyoyararlanımının Değerlendirilmesi (Project no: 1919B012305356)”, which was supported under the “TÜBİTAK–2209-A Üniversite Öğrencileri Araştırma Projeleri Desteği Programı”.
References
- Aghili, F., Khoshgoftarmanesh, A. H., Afyuni, M., Mobli, M. (2009). Relationships between fruit mineral nutrients concentrations and some fruit quality attributes in greenhouse cucumber. Journal of Plant Nutrition, 32(12), 1994–2007. https://doi.org/10.1080/01904160903308119
- Amalraj, A., Pius, A. (2015). In vitro study on the bioavailability of calcium and its absorption inhibitors in raw and cooked pulses commonly consumed in India. International Food Research Journal, 22(4), 1525–1532.
- Apaydın, H., Demirci, M., Bölük, E., Kopuk, B., Palabiyik, I. (2024). Effect of different roasting conditions on the physicochemical properties, acrylamide concentration, and mineral bioaccessibility of nuts. Food Bioscience, 58, 103646. https://doi.org/10.1016/j.fbio.2024.103646
- Aribas, M., Kahraman, K., Koksel, H. (2020). Effects of resistant starch type 4 supplementation of bread on in vitro glycemic index value, bile acid-binding capacity, and mineral bioavailability. Cereal Chemistry, 97(2), 163–171. https://doi.org/10.1002/cche.10229
- Atta-Aly, M. A. (1999). Effect of nickel addition on the yield and quality of parsley leaves. Scientia Horticulturae, 82(1–2), 9–24. https://doi.org/ 10.1016/S0304-4238(99)00032-1
- Buturi, C. V., Mauro, R. P., Fogliano, V., Leonardi, C., Giuffrida, F. (2021). Mineral biofortification of vegetables as a tool to improve human diet. Foods, 10(2), 1–23. https://doi.org/ 10.3390/foods10020223
- Ceccanti, C., Guidi, L., D’Alessandro, C., Cupisti, A. (2022). Potassium Bioaccessibility in Uncooked and Cooked Plant Foods: Results from a Static In Vitro Digestion Methodology. Toxins, 14(10). https://doi.org/10.3390/toxins14100668
- Choleva, T. G., Tziasiou, C., Gouma, V., Vlessidis, A. G., Giokas, D. L. (2023). In Vitro Assessment of the Physiologically Relevant Oral Bioaccessibility of Metallic Elements in Edible Herbs Using the Unified Bioaccessibility Protocol. Molecules, 28(14). https://doi.org/ 10.3390/molecules28145396
Details
Primary Language
English
Subjects
Food Nutritional Balance, Food Engineering
Journal Section
Research Article
Publication Date
October 15, 2025
Submission Date
March 4, 2025
Acceptance Date
August 30, 2025
Published in Issue
Year 2025 Volume: 50 Number: 5
APA
Apaydin, H., & Afşar, A. S. (2025). EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES. Gıda, 50(5), 713-722. https://doi.org/10.15237/gida.GD25037
AMA
1.Apaydin H, Afşar AS. EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES. The Journal of Food. 2025;50(5):713-722. doi:10.15237/gida.GD25037
Chicago
Apaydin, Hakan, and Ahmet Serhat Afşar. 2025. “EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES”. Gıda 50 (5): 713-22. https://doi.org/10.15237/gida.GD25037.
EndNote
Apaydin H, Afşar AS (October 1, 2025) EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES. Gıda 50 5 713–722.
IEEE
[1]H. Apaydin and A. S. Afşar, “EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES”, The Journal of Food, vol. 50, no. 5, pp. 713–722, Oct. 2025, doi: 10.15237/gida.GD25037.
ISNAD
Apaydin, Hakan - Afşar, Ahmet Serhat. “EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES”. Gıda 50/5 (October 1, 2025): 713-722. https://doi.org/10.15237/gida.GD25037.
JAMA
1.Apaydin H, Afşar AS. EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES. The Journal of Food. 2025;50:713–722.
MLA
Apaydin, Hakan, and Ahmet Serhat Afşar. “EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES”. Gıda, vol. 50, no. 5, Oct. 2025, pp. 713-22, doi:10.15237/gida.GD25037.
Vancouver
1.Hakan Apaydin, Ahmet Serhat Afşar. EVALUATION OF IN-VITRO MINERAL BIOACCESSIBILITY OF COMMONLY CONSUMED VEGETABLES. The Journal of Food. 2025 Oct. 1;50(5):713-22. doi:10.15237/gida.GD25037