B. CEREUS BİYOFİLMLERİNİN SİTRİK ASİT UYGULAMALARI İLE KONTROLÜ

Abstract

Bu çalışmada, önemli bir gıda patojeni olan B. cereus vejetatif hücrelerinin ve sporlarının mikrotitrasyon plaklarında, glukoz ve süt içeren besiyerleri kullanılarak (TSB_G ve TSB_S) oluşturduğu 24-72 saatlik biyofilmlerinin, %2 sitrik asit ve 200 ppm klor uygulamaları ile önlenmesi ve ortadan kaldırılması araştırılmıştır. B. cereus vejetatif hücrelerinin TSB_G ve TSB_S besiyerleri kullanıldığında oluşan biyofilmlerinin, sitrik asit uygulamaları ile %59 oranında önlendiği ve %38-63 oranlarında giderildiği belirlenmiştir. B. cereus spor biyofilmlerinin ise, %56 oranında önlenebildiği ve %40-56 oranlarında giderilebildiği görülmüştür. Sitrik asit uygulamasının (%2) oluşan B. cereus vejetatif hücrelerinin ve sporlarının biyofilmleri üzerinde klor kadar etkili olabildiği tespit edilmiştir.

Keywords

• B. cereus,biyofilm önlenmesi,biyofilm ortadan kaldırılması,sitrik asit,B. cereus sporları

CONTROL OF B. CEREUS BIOFILMS BY CITRIC ACID TREATMENTS

Abstract

In this work, the prevention and removal of biofilm formations of B. cereus vegetative cells and spore formations by citric acid (2%) and chlorine (200 ppm) treatments on microtitration plates were investigated. The biofilms were produced in the presence of glucose and milk (TSB_G and TSB_M) for 24-72 hours. B. cereus biofilms, formed by vegetative cells in TSB_G and TSB_M were inhibited by citric acid treatments by up to 59% and removed about 38-63%, respectively. However, biofilms of B. cereus spores were prevented about 56% and removed by 40-56%. It was shown that citric acid treatment (2%) could be as effective as chlorine treatment for biofilms of B. cereus vegetative cells and spores. 

Keywords

• prevention of biofilm,removal of biofilm,citric acid,B. cereus,spores of B. cereus

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