YER FISTIĞI KABUĞUNDAN BİYOETANOL ÜRETİMİ

Abstract

İkinci nesil biyoyakıtların önemi her geçen gün artmaktadır. Yer fıstığı ülkemizde bol miktarda yetişen bir tarım ürünüdür. Lignoselülozik bir tarımsal atık olan yer fıstığı kabuğu ikinci nesil biyoyakıt üretiminde biyokütle olarak kullanılabilmektedir. Yapılan deneylerde, üretilen biyoetanolü optimize etmek amacıyla farklı kabuk konsantrasyonları, hidroliz koşulları, Accellerase 1500 enzim kullanımı, inkübasyon koşulları, inokülasyon oranı ve farklı azot kaynakları denenmiştir. Denemeler sonucunda, enzimatik hidrolizle %10 kabuk ve 1 g/L soya unu ile hazırlanan besiyerlerinde, 12 saatte, aerobik koşullarda ve maya optik yoğunluğu 0.86 olduğunda etanol üretiminin 2.24 g/L olduğu belirlenmiştir. İnokülasyon oranı ve inkübasyon süresi arttığında maya gelişimi OD₆₀₀: 6.0’a kadar yükselmiş ancak etanol üretimi düşmüştür.

Keywords

• Yer fıstığı kabuğu,biyoetanol,yenilenebilir enerji,biyokütle

BIOETHANOL PRODUCTION FROM PEANUT SHELL

Abstract

The importance of second generation biofuels is increasing day by day. Peanut is an agricultural product that is abundantly produced in Turkey. Peanut shell which is a lignocellulosic agricultural biomass may be used to produce biofuel. In experiments to optimise bioethanol production, different shell loading concentrations, pretreatment conditions, the usage of Accellerase 1500 enzyme, incubation conditions, inoculation ratios and different nitrogen source were studied. In the media preparing with peanut shell sugar, obtaining after enzymatic hydrolysis, the bioethanol concentration and yeast growth was obtained as 2.24 g/L and 0.86 (OD₆₀₀) in the presence of 10% shell loading and 1 g/L soy flour at the end of 12 hours  incubation time in aerobic conditions. Higher yeast growth such as 6.0 (OD₆₀₀), lower bioethanol production were obtained when the inoculation ratio and incubation times were increased.

Keywords

• Peanut shell,bioethanol,renewable energy,biomass

References

1. Aguilar-Reynosa, A., Romaní, A., Rodríguez-Jasso, R.M., Aguilar, C.N., Garrote, G., Ruiz, H.A., (2017). Comparison of microwave and conduction-convection heating autohydrolysis pretreatment for bioethanol production. Bioresour. Technol. 243, 273–283. https://doi.org/10.1016/j.biortech.2017.06.096
2. Arı, T., Dönmez, S., (1999). Melastan Etil Alkol Üretiminde Soya Ununun Alkol ve Hücre Konsantrasyonuna Etkisi. Turkish J. Biol. 24, 573–584.
3. Bely, M., Sablayrolles, J.-M., Barre, P., (1990). Automatic detection of assimilable nitrogen deficiencies during alcoholic fermentation in oenological conditions. J. Ferment. Bioeng. 70, 246–252. https://doi.org/10.1016/0922-338X(90)90057-4
4. Carneiro, A.P., Rodr�guez, O., Macedo, E.A., (2017). Dissolution and fractionation of nut shells in ionic liquids. Bioresour. Technol. https://doi.org/10.1016/j.biortech.2016.11.112
5. Cho, D.H., Shin, S.J., Bae, Y., Park, C., Kim, Y.H., (2011). Ethanol production from acid hydrolysates based on the construction and demolition wood waste using Pichia stipitis. Bioresour. Technol. 102, 4439–4443. https://doi.org/10.1016/j.biortech.2010.12.094
6. Dadi, A.P., Varanasi, S., Schall, C.A., (2006). Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step. Biotechnol. Bioeng. 95, 904–910. https://doi.org/10.1002/bit.21047
7. Demirbaş, A., (1999). Properties of charcoal derived from hazelnut shell and the production of briquettes using pyrolytic oil. Energy 24, 141–150. https://doi.org/10.1016/S0360-5442(98)00077-2
8. Dussán, K.J., Silva, D.D. V, Moraes, E.J.C., Priscila, V., Felipe, M.G. a, (2014). Dilute-acid Hydrolysis of Cellulose to Glucose from Sugarcane Bagasse. Chem. Eng. Trans. 38, 433–438. https://doi.org/10.3303/CET1438073

9. Frei, M., (2013). Lignin: Characterization of a multifaceted crop component. Sci. World J. 2013. https://doi.org/10.1155/2013/436517
10. García-Ríos, E., Gutiérrez, A., Salvadó, Z.Z., Arroyo-López, F.N., Guillamon, J.M., (2014). The Fitness Advantage of Commercial Wine Yeasts in Relation to the Nitrogen Concentration, Temperature, and Ethanol Content under Microvinification Conditions. Appl. Environ. Microbiol. 80, 704–713. https://doi.org/10.1128/AEM.03405-13
11. Leitner, V., Lindorfer, J., (2016). Evaluation of technology structure based on energy yield from wheat straw for combined bioethanol and biomethane facility. Renew. Energy 87, 193–202. https://doi.org/10.1016/j.renene.2015.09.037
12. Martín, C., Thomsen, A.B., (2007). Wet oxidation pretreatment of lignocellulosic residues of sugarcane, rice, cassava and peanuts for ethanol production. J. Chem. Technol. Biotechnol. 82, 174–181. https://doi.org/10.1002/jctb.1648
13. Naz, A.A., Reinert, S., Bostanci, C., Seperi, B., Leon, J., Böttger, C., Südekum, K.-H., Frei, M., (2017). Mining the global diversity for bioenergy traits of barley straw: Genomewide association study under varying plant water status. GCB Bioenergy. https://doi.org/10.1111/gcbb.12433
14. Polachini, T.C., Sato, A.C.K., Cunha, R.L., Telis-Romero, J., (2016). Density and rheology of acid suspensions of peanut waste in different conditions: An engineering basis for bioethanol production. Powder Technol. 294, 168–176. https://doi.org/10.1016/j.powtec.2016.02.022
15. Putra, M.D., Abasaeed, A.E., Atiyeh, H.K., Al-Zahrani, S.M., Gaily, M.H., Sulieman, A.K., Zeinelabdeen, M.A., (2015). Kinetic Modeling and Enhanced Production of Fructose and Ethanol From Date Fruit Extract. Chem. Eng. Commun. 202, 1618–1627. https://doi.org/10.1080/00986445.2014.968711
16. Rouhollah, H., Iraj, N., Giti, E., Sorah, A., (2007). Mixed sugar fermentation by Pichia stipitis , Sacharomyces cerevisiaea , and an isolated xylose- fermenting Kluyveromyces marxianus and their cocultures. African J. Biotechnol. 6, 1110–1114.
17. Saha, B.C., Cotta, M.A., (2006). Ethanol production from alkaline peroxide pretreated enzymatically saccharified wheat straw. Biotechnol. Prog. 22, 449–453. https://doi.org/10.1021/bp050310r
18. Soccol, C.R., Vandenberghe, L.P. de S., Medeiros, A.B.P., Karp, S.G., Buckeridge, M., Ramos, L.P., Pitarelo, A.P., Ferreira-Leitão, V., Gottschalk, L.M.F., Ferrara, M.A., Silva Bon, E.P. da, Moraes, L.M.P. de, Araújo, J. de A., Torres, F.A.G., (2010). Bioethanol from lignocelluloses: Status and perspectives in Brazil. Bioresour. Technol. 101, 4820–4825. https://doi.org/10.1016/j.biortech.2009.11.067
19. Trigueros, D.E.G., Fiorese, M.L., Kroumov, A.D., Hinterholz, C.L., Nadai, B.L., Assunção, G.M., (2016). Medium optimization and kinetics modeling for the fermentation of hydrolyzed cheese whey permeate as a substrate for Saccharomyces cerevisiae var. boulardii. Biochem. Eng. J. 110, 71–83. https://doi.org/10.1016/j.bej.2016.02.014
20. Tuck, C.O., Pérez, E., Horváth, I.T., Sheldon, R.A., Poliakoff, M., (2012). Valorization of biomass: Deriving more value from waste. Science (80-. ). https://doi.org/10.1126/science.1218930
21. Wang, W., Kang, L., Wei, H., Arora, R., Lee, Y.Y., (2011). Study on the decreased sugar yield in enzymatic hydrolysis of cellulosic substrate at high solid loading. Appl. Biochem. Biotechnol. 164, 1139–1149. https://doi.org/10.1007/s12010-011-9200-8