EFFECT OF H2O2 OXIDATION STRESS ON CAROTENOID PRODUCTION IN CHLAMYDOMONAS REINHARDTII AND ITS ANTIOXIDANT ACTIVITY

Abstract

The effect of H2O2 oxidation stress on carotenoid production in C. reinhardtii and its antioxidant properties were investigated in this study. For this purpose, the amount of carotenoids determined by HPLC-DAD, total of phenolic contents and antioxidant capacities determined by Folin and CHROMAC methods respectively, in different oxidative stress conditions were studied. When the concentration of H2O2 was increased during the incubation period, total of phenolic content and antioxidant capacity value were decreased. In the same incubation period, HPLC-PDA results showed highest amounts of carotenoids in microalgae exposed to 1 μM H2O2 oxidative stress and it was thought that toxic dose might be in 20 μM oxidation media for microalgae. Thus, when the oxidative stress conditions were changed, the amounts of carotenoids and the structure of carotenoids could be changed. This study is important that the relationship between carotenoid and the power of oxidation stress in microalgae system.

Keywords

• Antioxidant
• Chlamydomonas reinhardtii
• Ultrasonic-assisted extraction
• HPLC
• Carotenoid
• oxidative stress

H2O2 OKSİDASYON STRESİNİN CHLAMYDOMONAS REINHARDTİİ MİKROALGİNİN KAROTENOİD ÜRETİMİ VE ANTİOKSİDAN AKTİVİTESI ÜZERİNE ETKİSİ

Öz

Bu çalışmada C. reinhardtii mikroalginin karotenoid üretimi ve antioksidan özellikleri üzerine H2O2 molekülünün oksidasyon etkisi incelenmiştir. Bu amaçla karotenoid miktarları HPLC-DAD cihazı ile, toplam fenolik madde ve antioksidan kapasite miktarları sırasıyla Folin ve CHROMAC yöntemleri ile tayin edilmiştir. Oksidatif stress süresi arttıkça karotenoid miktarlarında da artma görülmüştür. İnkübasyon süresi boyunca H2O2 konsantrasyonu arttırıldığında, toplam fenolik madde ve antioksidan kapasite değeri azalmıştır. Aynı inkübasyon süresi boyunca HPLC-DAD sonuçlarına göre, 1 μM H2O2 oksidatif strese maruz kalan mikroalglerde en yüksek miktarda karotenoid sentezlendiği ve mikroalgler için toksik dozun 20 μM oksidasyon ortamında olabileceği anlaşılmıştır. Böylece, oksidatif stres koşulları değiştiğinde, karotenoidlerin miktarları ve karotenoidlerin yapısı değiştirilebilir. Bu çalışma, mikroalg sisteminde, karotenoid sentezi ile oksidasyon stresi arasındaki ilişkinin açıklanması açısından önemlidir.

Anahtar Kelimeler

• Antioksidan
• Chlamydomonas reinhardtii
• Ultrasonik destekli ekstraksiyon
• HPLC
• Karotenoid
• Oksidatif stres

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