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SIĞIR LAKTOFERRİNİN ÇİĞ SÜTÜN MİKROBİYOLOJİK ÖZELLİKLERİNE ETKİSİ

Year 2021, , 681 - 691, 15.05.2021
https://doi.org/10.15237/gida.GD21041

Abstract

Sütler elde edilme şekline göre kontaminasyona gayet açık bir gıda maddesidir. Aynı zamanda içerdikleri besin bileşenleri yönünden ise mikrobiyolojik olarak çok hızlı bozulabilen bir üründür. Gerek mevzuatlar yönünden gerekse bilinçli tüketim yönünden çiğ sütlerin işletmelerde işlemeye alınma sürecine kadar çok fazla müdahele edilememektedir. Bu nedenle çiğ sütlerde başta Pseudomonas spp. olmak üzere Koliformlar ve Laktik asit bakterilerin çoğalması nedeniyle gerek üründe gerekse teknolojide çeşitli sıkıntılar ortaya çıkabilmektedir. Çalışmamızda doğal bir inhibitör madde olan laktoferrin ilavesi ile soğuk zincirde tutulan çiğ sütlerin mikrobiyolojik özelliklerine etkisi incelenmiştir. Çalışma neticesinde laktoferrinin laktik asit bakterleri üzerine önemli düzeyde (p <0.05) inhibe edici bir etkisi görülmemiştir. Ancak Pseudomonas spp. ve Koliform bakterilerin çoğalmalarını engelleyerek önemli düzeyde (p <0.05) antibakteriyel etki göstermiştir. Laktoferrin konsantrasyonu arttıkça antimikrobiyal etki de önemli düzeyde artmıştır. Bu durum laktoferrinin sıvı gıdaların soğuk sistemlerde doğal bir antimikrobiyal ajan olarak kullanılabileceğini göstermiştir.

Supporting Institution

Aksaray Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2015-016

Thanks

Aksaray Üniversitesi Bilimsel Araştırma Projelerine (Proje No: 2015-016) bu çalışmanın büyük bir kısmına verdikleri destek için teşekkür ederiz.

References

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  • Al-Nabulsi, A.A., Holley, R.A., (2007). Effects of Escherichia coli O157:H7 and meat starter cultures of bovine lactoferrin in broth and microencapsulated lactoferrin in dry sausage batters, International Journal of Food Microbiology. 113: 84–91, https://doi.org/10.1016/j.ijfoodmicro.2006.07.019.
  • Alkın, E. (2008). Laktoferrin ve gıdalarda kullanımı. Gıda ve Yem Bilimi Teknolojisi, 10: 31-38.
  • APHA (1992). In: Standard Methods for the Examination of Dairy Products, Marshall, R.T. (Ed)., 16th ed., American Public Health Association. Washington, DC., p.546.
  • Appelmelk, BJ., Yun-qing, A., Geerts, M., Thijs, BG., Boer, HA., Maclaren, DM., Graaff, J., Nuijens, JH. (1994) Lactoferrin is a lipid A-binding protein. Infect. Immun. 62: 2628-2632.
  • Avcı, G. (2007). Laktoferrinin Biyolojik Özellikleri ve Hastalıklarla İlişkisi. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 7 (1): 23-34.
  • Barbiroli, A., Bonomi, F., Capretti., Iematti, S., Manzoni, M., Piergiovanni L., Rollini, M. (2012). Antimicrobial activity of lysozyme and lactoferrin incorporated in cellulose-based food packaging. Food Control, 26: 387-392, https://doi.org/10.1016/j.foodcont.2012.01.046.
  • Branen, J. and Davidson, P.M. (2000) Activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: the effect of EDTA. Letters of Applied Microbiology, 30: 233–237, https://doi.org/10.1046/j.1472-765x.2000.00711.x.
  • Bravo, D., de Alba, M., Medina, M. (2014). Combined treatments of high-pressure with the lactoperoxidase system or lactoferrin on the inactivation of Listeria monocytogenes, Salmonella enteritidis and Escherichia coli O157:H7 in beef carpaccio. Food Microbiology, 41: 27-32, https://doi.org/10.1016/j.fm.2014.01.010.
  • Brooks, J.D., Flint, S.H. (2008). Biofilms in the food industry: problems and potential solutions, International Journal of Food Science and Technology, 43: 2163–2176, https://doi.org/10.1111/j.1365-2621.2008.01839.x.
  • Chen, P.W.; Jheng, T.T.; Shyu, C.L.; Mao, F.C. (2013). Antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. J. Dairy Sci., 96: 1438–1446. https://doi.org/10.3168/jds.2012-6112.
  • Colak, H., Hampikyan, H., Bingol, E.B., Aksu, H., (2008). The effect of nisin and bovine lactoferrin on the microbiological quality of turkish-style meatball (tekirdağ köfte), Journal of Food Safety, 28: 355–375, https://doi.org/10.1111/j.1745-4565.2008.00105.x.
  • Del Olmo, A., Calzada, Nunez, M. (2012). Effect of lactoferrin and its derivatives, high hydrostatic pressure, and their combinations, on Escherichia coli O157:H7 and Pseudomonas fluorescens in chicken filets. Innovative Food Science & Emerging Technologies. 13: 51–56, https://doi.org/10.1016/j.ifset.2011.07.016.
  • Duran, A., Kahve, H.I. (2017). The use of lactoferrin in food industry, Academic Journal of Science, 07 (02): 89-94.
  • EFSA (2012). Scientific opinion on bovine lactoferrin, European Food Safety Authority Journal, Vol. 10, Iss. 5, https://doi.org/10.2903/j.efsa.2012.2701.
  • Enrique, M., Marcos, J.F., Yuste, M., Martínez, M., Vallés, S., Manzanares, P., (2008). Inhibition of the wine spoilage yeast Dekkera bruxellensis by bovine lactoferrin-derived peptides, International Journal of Food Microbiology, 127: 229–234, https://doi.org/10.1016/j.ijfoodmicro.2008.07.011.
  • Erga, K.S., Peen, E., Tenstad, O., Reed, R.K. (2000). Lactoferrin and anti‐lactoferrin antibodies: Effects of ironloading of lactoferrin on albumin extravasation in different tissues in rats, Acta Physiologica Scandinavica. 170 (1): 11-19, https://doi.org/10.1046/j.1365-201x.2000.00754.x.
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  • Giraffa, G. (2012). Selection and design of lactic acid bacteria probiotic cultures. Eng. Life Sci, 12 (4): 391–398. https://doi.org/10.1002/elsc.201100118.
  • Gonzalez-Chavez, S.A., Arevalo Gallegos, S., Roscon Cruz, Q. (2009). Lactoferrin: structure, function and applications. International Journal of Antimicrobial Agents, 33 (4): 301.e1–301.e8, https://doi.org/10.1016/j.ijantimicag.2008.07.020.
  • Gür, F., Güzel, M., Öncül, N., Yıldırım, Z., Yıldırım M. (2010). Süt serum proteinleri ve türevlerinin biyolojik ve fizyolojik aktiviteleri, Akademik Gıda, 8 (1): 23-31.
  • Hector, A., Kröner, C., Carevic, M., Bakele, M., Rieber, N., Riethmüller, J., Griese, M., Zissel, G., Hartl, D. (2014). The chemokine CCL18 characterises Pseudomonas infections in cystic fibrosis lung disease. Eur Respir J., 44: 1608-1615, https://doi.org/10.1183/09031936.00070014.
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  • ISO/TS 11059 (2009). Milk and milk products. Method for the enumeration of Pseudomonas spp., International Standardization Organization ed., Geneva, Switzerland.
  • Jenssen, H., Hancock R. (2009). Antimicrobial properties of lactoferrin. Biochimie, 91 (1): 19-29, https://doi.org/10.1016/j.biochi.2008.05.015
  • Kamiya, H., Ehara, T. ve Matsumoto T., (2012). Inhibitory effects of lactoferrin on biofilm formation in clinical isolates of Pseudomonas aeruginosa. J Infect Chemother. 18: 47-52. https://doi.org/10.1007/s10156-011-0287-1.
  • Kim, WS., Ohashi, M., Shimazaki K. (2016). Inhibitory effects of synthetic peptides containing bovine lactoferrin C-lobe sequence on bacterial growth, Korean J Food Sci Anim Resour., 36(4): 452-457. https://doi.org/10.5851/kosfa.2016.36.4.452.
  • Komine, K., Komine, Y., Kuroishi, T., Kobayashi, J., Obara, Y., Kumagai, K. (2005). Small molecule lactoferrin with an inflammatory effect but no apparent antibacterial activity in mastitic mammary gland secretion. J. Vet. Med. Sci. 67: 667–677. https://doi.org/10.1292/jvms.67.667.
  • Kutila, T., Pyörälä, S., Saloniemi, H., Kaartinen, L. (2003). Antibacterial effect of bovine lactoferrin against udder pathogens, Acta Veterinaria Scandinavica, 44: 35-42, https://doi.org/10.1186/1751-0147-44-35.
  • Matijašic, BB., Oberckal, J., Lorbeg, PM., Paveljšek, D., Skale, N., Kolenc, B., Gruden, Š., Ulrih, NP., Kete M., Justin MZ. (2020). Characterisation of lactoferrin ısolated from acid whey using pilot-scale monolithic ion-exchange chromatography, Processes, 8 (7): 804, https://doi.org/10.3390/pr8070804.
  • Meier, F., Lacroix, C., Meile, L., Jans, C. (2018). Enterococci and pseudomonads as quality indicators in industrial production and storage of mozzarella cheese from raw cow milk, International Dairy Journal, 82: 28e34, https://doi.org/10.1016/j.idairyj.2018.02.010.
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THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK

Year 2021, , 681 - 691, 15.05.2021
https://doi.org/10.15237/gida.GD21041

Abstract

Milk is a foodstuff that is very open to contamination depending on the way it is obtained. Also, it is a microbiologically perishable product in terms of the nutritional components it contains. Regarding both regulations and consumer awareness, it cannot be intervened substantially until the process of raw milk in the dairy farms. Therefore, due to the proliferation of Coliforms, especially Pseudomonas spp. and lactic acid bacteria, various problems may arise in both product and technology. The present study aimed to investigate the effect of lactoferrin, a natural inhibitor, on the microbiological properties of raw milk kept in the cold chain. As a result of the study, no inhibitory effect of lactoferrin on lactic acid bacteria was observed. However, it showed a significant antibacterial effect by inhibiting the growth of Pseudomonas spp. and Coliform (P<0.05). As the lactoferrin concentration increased, the antimicrobial effect increased significantly. This showed that lactoferrin can be used as a natural antimicrobial agent in cold systems of liquid foods.

Project Number

2015-016

References

  • Aksan, E, (2017). Gıdaların mikrobiyal bozulması. In: Gıda mikrobiyolojisi, Erkmen, O. (Ed), 5th ed., Efil Yayınevi, Ankara, Turkey, s.82-123.
  • Al-Nabulsi, A.A., Holley, R.A., (2007). Effects of Escherichia coli O157:H7 and meat starter cultures of bovine lactoferrin in broth and microencapsulated lactoferrin in dry sausage batters, International Journal of Food Microbiology. 113: 84–91, https://doi.org/10.1016/j.ijfoodmicro.2006.07.019.
  • Alkın, E. (2008). Laktoferrin ve gıdalarda kullanımı. Gıda ve Yem Bilimi Teknolojisi, 10: 31-38.
  • APHA (1992). In: Standard Methods for the Examination of Dairy Products, Marshall, R.T. (Ed)., 16th ed., American Public Health Association. Washington, DC., p.546.
  • Appelmelk, BJ., Yun-qing, A., Geerts, M., Thijs, BG., Boer, HA., Maclaren, DM., Graaff, J., Nuijens, JH. (1994) Lactoferrin is a lipid A-binding protein. Infect. Immun. 62: 2628-2632.
  • Avcı, G. (2007). Laktoferrinin Biyolojik Özellikleri ve Hastalıklarla İlişkisi. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 7 (1): 23-34.
  • Barbiroli, A., Bonomi, F., Capretti., Iematti, S., Manzoni, M., Piergiovanni L., Rollini, M. (2012). Antimicrobial activity of lysozyme and lactoferrin incorporated in cellulose-based food packaging. Food Control, 26: 387-392, https://doi.org/10.1016/j.foodcont.2012.01.046.
  • Branen, J. and Davidson, P.M. (2000) Activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: the effect of EDTA. Letters of Applied Microbiology, 30: 233–237, https://doi.org/10.1046/j.1472-765x.2000.00711.x.
  • Bravo, D., de Alba, M., Medina, M. (2014). Combined treatments of high-pressure with the lactoperoxidase system or lactoferrin on the inactivation of Listeria monocytogenes, Salmonella enteritidis and Escherichia coli O157:H7 in beef carpaccio. Food Microbiology, 41: 27-32, https://doi.org/10.1016/j.fm.2014.01.010.
  • Brooks, J.D., Flint, S.H. (2008). Biofilms in the food industry: problems and potential solutions, International Journal of Food Science and Technology, 43: 2163–2176, https://doi.org/10.1111/j.1365-2621.2008.01839.x.
  • Chen, P.W.; Jheng, T.T.; Shyu, C.L.; Mao, F.C. (2013). Antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. J. Dairy Sci., 96: 1438–1446. https://doi.org/10.3168/jds.2012-6112.
  • Colak, H., Hampikyan, H., Bingol, E.B., Aksu, H., (2008). The effect of nisin and bovine lactoferrin on the microbiological quality of turkish-style meatball (tekirdağ köfte), Journal of Food Safety, 28: 355–375, https://doi.org/10.1111/j.1745-4565.2008.00105.x.
  • Del Olmo, A., Calzada, Nunez, M. (2012). Effect of lactoferrin and its derivatives, high hydrostatic pressure, and their combinations, on Escherichia coli O157:H7 and Pseudomonas fluorescens in chicken filets. Innovative Food Science & Emerging Technologies. 13: 51–56, https://doi.org/10.1016/j.ifset.2011.07.016.
  • Duran, A., Kahve, H.I. (2017). The use of lactoferrin in food industry, Academic Journal of Science, 07 (02): 89-94.
  • EFSA (2012). Scientific opinion on bovine lactoferrin, European Food Safety Authority Journal, Vol. 10, Iss. 5, https://doi.org/10.2903/j.efsa.2012.2701.
  • Enrique, M., Marcos, J.F., Yuste, M., Martínez, M., Vallés, S., Manzanares, P., (2008). Inhibition of the wine spoilage yeast Dekkera bruxellensis by bovine lactoferrin-derived peptides, International Journal of Food Microbiology, 127: 229–234, https://doi.org/10.1016/j.ijfoodmicro.2008.07.011.
  • Erga, K.S., Peen, E., Tenstad, O., Reed, R.K. (2000). Lactoferrin and anti‐lactoferrin antibodies: Effects of ironloading of lactoferrin on albumin extravasation in different tissues in rats, Acta Physiologica Scandinavica. 170 (1): 11-19, https://doi.org/10.1046/j.1365-201x.2000.00754.x.
  • Ergüllü, E., Üçüncü, M. (1982). Farklı sıcaklıkta bırakılan çiğ sütlerde mikroorganizma sayısı bakımından ortaya çıkan değişimler üzerinde araştırmalar, Gıda. 7 (3): 93-97.
  • Giraffa, G. (2012). Selection and design of lactic acid bacteria probiotic cultures. Eng. Life Sci, 12 (4): 391–398. https://doi.org/10.1002/elsc.201100118.
  • Gonzalez-Chavez, S.A., Arevalo Gallegos, S., Roscon Cruz, Q. (2009). Lactoferrin: structure, function and applications. International Journal of Antimicrobial Agents, 33 (4): 301.e1–301.e8, https://doi.org/10.1016/j.ijantimicag.2008.07.020.
  • Gür, F., Güzel, M., Öncül, N., Yıldırım, Z., Yıldırım M. (2010). Süt serum proteinleri ve türevlerinin biyolojik ve fizyolojik aktiviteleri, Akademik Gıda, 8 (1): 23-31.
  • Hector, A., Kröner, C., Carevic, M., Bakele, M., Rieber, N., Riethmüller, J., Griese, M., Zissel, G., Hartl, D. (2014). The chemokine CCL18 characterises Pseudomonas infections in cystic fibrosis lung disease. Eur Respir J., 44: 1608-1615, https://doi.org/10.1183/09031936.00070014.
  • ISO 15214 (1998). Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of mesophilic lactic acid bacteria. Colony-count technique at 30 ºC. International Organization for Standardization, Geneve, Switzerland.
  • ISO/FDIS 8086 (2004). Dairy plant - hygiene conditions - General guidance on inspection and sampling procedures. Final Draft international standard. Geneva, CH: International Organization for Standardization. Geneva, Switzerland.
  • ISO/TS 11059 (2009). Milk and milk products. Method for the enumeration of Pseudomonas spp., International Standardization Organization ed., Geneva, Switzerland.
  • Jenssen, H., Hancock R. (2009). Antimicrobial properties of lactoferrin. Biochimie, 91 (1): 19-29, https://doi.org/10.1016/j.biochi.2008.05.015
  • Kamiya, H., Ehara, T. ve Matsumoto T., (2012). Inhibitory effects of lactoferrin on biofilm formation in clinical isolates of Pseudomonas aeruginosa. J Infect Chemother. 18: 47-52. https://doi.org/10.1007/s10156-011-0287-1.
  • Kim, WS., Ohashi, M., Shimazaki K. (2016). Inhibitory effects of synthetic peptides containing bovine lactoferrin C-lobe sequence on bacterial growth, Korean J Food Sci Anim Resour., 36(4): 452-457. https://doi.org/10.5851/kosfa.2016.36.4.452.
  • Komine, K., Komine, Y., Kuroishi, T., Kobayashi, J., Obara, Y., Kumagai, K. (2005). Small molecule lactoferrin with an inflammatory effect but no apparent antibacterial activity in mastitic mammary gland secretion. J. Vet. Med. Sci. 67: 667–677. https://doi.org/10.1292/jvms.67.667.
  • Kutila, T., Pyörälä, S., Saloniemi, H., Kaartinen, L. (2003). Antibacterial effect of bovine lactoferrin against udder pathogens, Acta Veterinaria Scandinavica, 44: 35-42, https://doi.org/10.1186/1751-0147-44-35.
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  • Meier, F., Lacroix, C., Meile, L., Jans, C. (2018). Enterococci and pseudomonads as quality indicators in industrial production and storage of mozzarella cheese from raw cow milk, International Dairy Journal, 82: 28e34, https://doi.org/10.1016/j.idairyj.2018.02.010.
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There are 56 citations in total.

Details

Primary Language English
Subjects Food Engineering
Journal Section Articles
Authors

Ayhan Duran 0000-0002-4042-0988

Project Number 2015-016
Publication Date May 15, 2021
Published in Issue Year 2021

Cite

APA Duran, A. (2021). THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK. Gıda, 46(3), 681-691. https://doi.org/10.15237/gida.GD21041
AMA Duran A. THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK. GIDA. May 2021;46(3):681-691. doi:10.15237/gida.GD21041
Chicago Duran, Ayhan. “THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK”. Gıda 46, no. 3 (May 2021): 681-91. https://doi.org/10.15237/gida.GD21041.
EndNote Duran A (May 1, 2021) THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK. Gıda 46 3 681–691.
IEEE A. Duran, “THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK”, GIDA, vol. 46, no. 3, pp. 681–691, 2021, doi: 10.15237/gida.GD21041.
ISNAD Duran, Ayhan. “THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK”. Gıda 46/3 (May 2021), 681-691. https://doi.org/10.15237/gida.GD21041.
JAMA Duran A. THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK. GIDA. 2021;46:681–691.
MLA Duran, Ayhan. “THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK”. Gıda, vol. 46, no. 3, 2021, pp. 681-9, doi:10.15237/gida.GD21041.
Vancouver Duran A. THE EFFECT OF BOVINE LACTOFERRIN ON THE MICROBIOLOGICAL PROPERTIES OF RAW MILK. GIDA. 2021;46(3):681-9.

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