OPTIMIZATION OF THE EXTRACTION CONDITIONS OF PHENOLIC COMPOUNDS FROM ALCHEMILLA VULGARIS USING RESPONSE SURFACE METHODOLOGY

Abstract

The object of this study is to determine the best solvent and optimum extraction conditions for extraction of maximum phenolic compounds from Alchemilla vulgaris leaves. Extractions were carried out using solvents with different polarities. Box-Behnken Design was used to optimize extraction conditions including extraction time, temperature, and liquid/solid ratio. In the study, extract obtained with acetone-water indicated the highest total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity. Optimum extraction conditions for both responses were found as 168 min, 48.5 °C, and liquid/solid ratio of 41:1. The maximum TPC and TFC values were determined as 7.17 mg GAE/gdw and 3.63 mg QE/gdw under optimum extraction conditions. Concentrations of five phenolic compounds analyzed by HPLC increased significantl after optimization. The results indicated that optimizing extraction conditions is critical for quantification of phenolic compounds. The present model can contribute to food industry where phenolic compounds have potential use as biopreservatives.

Keywords

• Alchemilla vulgaris
• Antioxidant activity
• Box-Behnken Design
• Extraction optimization
• Phenolic compounds

ALCHEMILLA VULGARIS'TEN FENOLİK BİLEŞİKLERİN EKSTRAKSİYON KOŞULLARININ YANIT YÜZEY YÖNTEMİ KULLANILARAK OPTİMİZASYONU

Abstract

Bu çalışmanın amacı, Alchemilla vulgaris bitkisinin yapraklarından maksimum fenolik bileşik ekstraksiyonu için en iyi çözücünün ve optimum ekstraksiyon koşullarının belirlenmesidir. Ekstraksiyonlar, farklı polaritelere sahip çözücüler kullanılarak gerçekleştirildi. Box-Behnken tasarımı (BBD), ekstraksiyon süresi, ekstraksiyon sıcaklığı ve sıvı / katı oranı içeren ekstraksiyon koşullarını optimize etmek için kullanıldı. Çalışmada aseton-su ile elde edilen ekstrakt, en yüksek toplam fenolik içerik (TPC), toplam flavonoid içerik (TFC) ve antioksidan aktivite göstermiştir. Her iki yanıt için optimum ekstraksiyon koşulları, 168 dakika, 48.5 ºC ve 41:1 sıvı / katı oranı olarak bulundu. Optimum ekstraksiyon koşulları altında maksimum TPC ve TFC değerleri 7.17 mg GAE / gdw ve 3.63 mg QE / gdw olarak belirlendi. HPLC ile analiz edilen beş fenolik bileşiğin konsantrasyonlarının, optimizasyondan sonra önemli ölçüde arttığı bulundu. Sonuçlar, ekstraksiyon koşullarının optimize edilmesinin fenolik bileşiklerin miktarının belirlenmesi için önemli olduğunu göstermektedir. Mevcut model, fenolik bileşiklerin biyo-koruyucu olarak potansiyel kullanıma sahip olduğu gıda endüstrisine katkıda bulunabilir.

Keywords

• Alchemilla vulgaris
• Antioksidan aktivite
• Box-Behnken Tasarımı
• Ekstraksiyon optimizasyonu
• Fenolik bileşikler

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