The Effects of UV-C Irradiation on Secondary Metabolite Production in Vitis Vinifera Cv. Gamay Callus Cultures (Turkish with English Abstract)

Abstract

In this study, the effects of UV-C irradiation on secondary metabolite accumulation in callus cultures of “Gamay” (Vitis vinifera L.) grape cultivar were investigated. Calli were obtained from petiole on B5 media added 0.5 mg/L BAP (benzyl amino purin), 0.5 mg/L IAA (indole acetic acid), 3% sucrose and 0.8% agar agar. UV-C was applied onto 12 days old calli at 10 and 15 cm distances from the source, for 10, 15 and 20 min. After treatment, calli were cultured in dark conditions at 25 oC and sampled after 0, 24 and 48 hours. Total phenolic compound, total flavanols, total flavonols, anthocyanin and carotenoid contents were determined by spectrophotometrically. All criteria were changed greatly depending on the combination of irradiation distance from UV-C source, treatment period and sampling time.  It was determined that UV-C irradiation had remarkable promoting effects on the accumulation of phenolics and carotenoid in the calli of Gamay grape cultivar.

Keywords

• Grapevine, callus, UV-C, phenolic compound, carotenoid

UV-C Uygulamasının Gamay Üzüm Çeşidine Ait Kalluslarda Sekonder Metabolit Üretimi Üzerine Etkileri

Abstract

Bu araştırmada Gamay (Vitis vinifera L.) üzüm çeşidine ait kallus kültürlerinde, UV-C uygulamasının sekonder metabolit birikimi üzerine olan etkileri incelenmiştir. Yaprak sapı eksplantlarından 0.5 mg/L BAP (benzil amino pürin), 0.5 mg/L (IAA) indol asetik asit, % 3 sakkaroz ve % 0.8 agar agardan oluşan B5 besin ortamında elde edilmiş olan 12 günlük kalluslara 10 ve 15 cm uzaklıktan 10, 15 ve 20 dakika süre ile 254 nm dalga boyuna sahip UV-C radyasyonu uygulaması yapılmıştır. Uygulamanın ardından kalluslar 25 °C’de ve karanlık koşullarda 0, 24 ve 48 saat süre ile inkübe edilmişlerdir. Bu süre sonunda alınan örneklerde toplam fenolik madde, toplam flavanol, toplam flavonol, antosiyanin ve karotenoit miktarları spektrofotometrik olarak belirlenmiştir. İncelenen tüm kriterlerin, UV-C radyasyonunun uygulama mesafesi, uygulama süresi ile örnek alım zamanlarından oluşan kombinasyonlara göre önemli ölçüde değiştiği belirlenmiştir. UV-C uygulamasının Gamay üzüm çeşidine ait kalluslarda fenolik bileşikler ve karotenoit birikimi üzerine dikkate değer ölçüde teşvik edici bir etkiye sahip olduğu tespit edilmiştir. 

Keywords

• Asma, kallus, UV-C, fenolik bileşik, karotenoit.

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