PROPOLİS EKSTRAKTI İLE ZENGİNLEŞTİRİLMİŞ BALIK YAĞINDA LİPİT OKSİDASYONU DÜZEYİNİN BELİRLENMESİ

Year 2018, Volume: 43 Issue: 3, 523 - 532, 18.05.2018

İlknur Uçak

https://doi.org/10.15237/gida.GD18031

Cited By: 4

Abstract

Bu çalışma, propolis ekstraktının balık yağında doğal
antioksidan olarak kullanımı üzerine odaklanmıştır. Farklı konsantrasyonlarda
(100, 500, 1.000 mg/kg)  propolis
ekstraktı ve sentetik antioksidan olarak bütillendirilmiş hidroksitoluen (BHT,
100 mg/kg) balık yağına eklenerek hızlandırılmış oksidasyon koşulları altında
lipit oksidasyonu düzeyi belirlenmiştir. Depolama süresince peroksit değeri,
UV-spektrum değerleri (UV₂₃₂ ve UV₂₇₀), tiyobarbitürik
asit (TBARS) değeri ve para-anisidin değeri incelenmiştir.  Depolama boyunca propolis ekstraktı ilaveli
gruplarda peroksit değeri, UV₂₃₂ ve UV₂₇₀ değeri, TBARS
değeri ve para-anisidin değeri kontrol grubu ve BHT ilaveli gruptan daha düşük
düzeylerde bulunmuştur. Elde edilen sonuçlar, 500 ve 1.000 mg/kg konsantrasyonlarında
propolis ekstraktı ilave edilmiş balık yağı örneklerinde oksidasyon düzeyinin
daha düşük olduğunu ve en etkili konsantrasyonun 1.000 mg/kg olduğunu
göstermektedir. Bu çalışma ile, propolis ekstraktının balık yağında oksidatif
stabiliteyi sağlamada doğal bir antioksidan kaynağı olarak kullanılabileceği
gösterilmektedir.

Keywords

Balık yağı, propolis ekstraktı, lipit oksidasyonu, doğal antioksidan, oksidatif bozulma

DETERMINATION OF THE LIPID OXIDATION LEVEL IN FISH OIL ENRICHED WITH PROPOLIS EXTRACT

Abstract

This study focused on the utilization of propolis extract
as a natural antioxidant in fish oil. Propolis extract at different
concentrations (100, 500, 1,000 mg/kg) and butylated hydroxytoluene (BHT, 100
mg/kg) as synthetic antioxidant were added to fish oil in order to determine
the lipid oxidation level under accelerated oxidation conditions. Peroxide
value, UV-spectrum values (UV₂₃₂ and UV₂₇₀),
thiobarbituric acid (TBARS) value and para-anisidine value were investigated
during the storage. Peroxide value, UV₂₃₂ and UV₂₇₀
value, TBARS value and para-anisidine value of groups which supplemented with
the propolis extract was found lower than the control group and BHT added group
throughout the storage period. The results show that the oxidation level in
fish oil samples supplemented with 500 and 1.000 mg/kg propolis extract lower,
and the most effective concentration is 1.000 mg/kg propolis extract. This
study indicated that the propolis extract might be used as a natural source in order
to provide oxidative stability in fish oil.

Keywords

Fish oil, propolis extract, lipid oxidation, natural antioxidant, oxidative deterioration

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