HIZLI Escherichia coli TANIMLANMASINDA ULTRAVİYOLE DESTEKLİ İNDOL VE MUG ESASLI TESTLERİN KIYASLANMASI

Year 2016, Volume: 41 Issue: 2, 85 - 90, 01.04.2016

Gizem Özlük Çilak Hilal Selamoğlu Derya Dülger Fadime Kurukama Ceren Çağlar Kadir Halkman

Abstract

Bu çalışmada önceki ISO standardında (ISO 9308-1:2000) belirtilen UV destekli indol esaslı hızlı E. colitesti ile Bactident E. coli testi (MUG esaslı hızlı test) karşılaştırılmıştır. Bu amaçla çeşitli gıda örneklerindenizole edilen ve tanısı yapılmış olan 46 adet E. coli izolatı, 4 referans E. coli suşu ile koliform grup bakterileringeliştirildiği besiyerlerinde tipik koliform kolonisi oluşturmasa da; gelişerek koloni oluşturabilen bireradet Salmonella Enteritidis ve Klebsiella oxytoca olmak üzere toplam 52 Enterobacteriaceae üyesibakteri her iki yöntemle analiz edilmiştir. Araştırma sonuçlarına göre MUG esaslı hızlı test %100 doğrusonuç verirken UV destekli indol esaslı testte %94.1 doğru sonuç alınmıştır. K. oxytoca, bu testte sorunaneden olmuştur. Laboratuvar koşularında Bactident E. coli kitinin kullanılması UV destekli indol testinekıyasla çok daha hızlı ve kolay olarak değerlendirilmiştir

Keywords

Escherichia coli, MUG, indol, hızlı tanımlama

THE COMPARISON OF RAPID Escherichia coli TESTS BASED ON MUG AND ULTRAVIOLET SUPPORTED INDOLE

Abstract

In this study, Bactident E. coli (rapid test based on MUG) and indole test supported by UV which wasindicated in previous ISO method (ISO 9308:2000) was compared. Both methods were applied on 46E. coli strains which were isolated from different food samples, 4 reference E. coli strains, 2 non-coliformbacteria as Salmonella Enteritidis and Klebsiella oxytoca which can be grown on the coliform media.46 E. coli isolates, isolated from various foods and identified by MUG and indole tests. According toresults accuracy of indole test supported by UV was determined as 94.1% whereas it was found 100%for Bactident E. coli. K. oxytoca has caused problem in UV supported indole test. In laboratory conditions,using Bactident E. coli is more rapid and easy then UV supported indole test

Keywords

Escherichia coli, MUG, indole, rapid identification

References

• Fricker CR, Bullock S, Murrin K, Niemela SI. 2008. Use of ISO 9308-1 Procedure for the detection of E. coli in water utilizing two incubation temperatures and two confirmation procedures; comparison with defined substrate technology (DST). J Water Health, 06 (3): 389-397.
• Pitkänen T, Paakkari P, Miettinen IT, Tanski HH, Paulin L, Hänninen ML. 2006. Comparison of media for enumeration of coliform bacteria and Escherichia coli in non-disinfected water. J Microbiol Methods, 68: 522-529.
• Taştemür S, Halkman AK. 2014. Membran filtrasyon tekniği ile koliform analizinde kullanılan besiyerlerinin kıyaslanması. GIDA, 39 (5): 267-273. 4. Bonadonna L, Cataldo C, Semproni M. 2007. Comparison of methods and confirmation tests for the recovery Escherichia coli in water. Desalination 213: 18-23.
• Farmer JJ. 1995. Enterobacteriaceae: Introduc- tion and Identification. In: Manual of Clinical Microbiology 6th Ed (Chief Ed. PR Murray). American Society for Microbiology ASM, Washington DC, USA, p 438-449.
• Çakır İ, 2000. Escherichia coli O157:H7. Gıda Mikrobiyolojisi ve Uygulamaları. Sim Matbaacılık, Ltd. Şti., Ankara, Türkiye. s. 403-411.
• ISO 9308-1:2014 Water Quality- Detection and Enumeration of Escherichia coli and coliform bacteria.
• Lange B, Strathmann M, Oßmer R. 2013. Performance validation of chromogenic coliform agar for the enumeration of Escherichia coli and coliform bacteria. Lett Appl Microbiol 57: 547-553.
• Schets FM, Nobel PJ, Strating S, Mooijman KA, Engels GB, Brouwer A. 2001. Comparison of methods for enumeration of total coliforms and Escherichia coli in water samples in the Netherlands. National Institute of Public Health and the Environment. RIVM report: 289202 028.
• ISO 9308-1:2000 Water Quality- Detection and Enumeration of Escherichia coli and coliform bacteria. Part 8.4: Rapid Test.
• Holbrook R, Anderson JM, Baird-Parker AC. 1980. Modified Direct Plate Method for Counting Escherichia coli in foods. Food Technol. 32: 78-83. 12. Clarke PH, Cowen ST. 1952. Biochemical Methods for Bacteriology. J Gen Microbiol, 6, 187 13. MacFaddin JF. 1985. Media for Isolation- Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
• Finegold SM, Baron EJ, Bailey WR, Scott EG. 1986. Diagn Microbiol, 7th Ed., The C.V. Mosby Co., St. Louis.
• Porubsky PR, Scott EE, Williams TD. 2008. p-Dimethylaminocinnamaldehyde Derivatization for Colorimetric Detection and HPLC-UV/Vis-MS/ MS Identification of Indoles. Arch Biochem Biophys. 475: 14–17.
• Niemi RM, Heikkilä MP, Lahti K, Kalso S, Niemelä SI. 2001. Comparison of methods for determining the numbers and species distribution of coliform bacteria in well water samples. J Appl Microbiol 90, 850-858.
• Halkman K (ed). 2005. Merck Gıda Mikrobiyolojisi Uygulamaları. Başak Matbaacılık ve Tanıtım Hizmetleri Ltd. Şti., 358 s., Ankara.
• Merck. 2010. Bactident E. coli, Test kit for the rapid identification of E. coli. Merck Microbiology Manual 12th Edition. s 180.
• Weber A, Schäfer R. 1989. The rapid identification of Escherichia coli using Bactident E. coli (Article in German). Berl Munch Tierarztl Wochenschr, 102(1): 4-5.
• Hansen W, Yourasowsky E. 1984. Detection of β-Glucuronidase in Lactose-Fermenting Members of the Family Enterobacteriaceae and its Presence in Bacterial Urine Cultures. J Clin Microbiol, 20; 1177-1179.
• www.mikrobiyoloji.org. Erişim tarihi: 17.08.2015