Türkiye’nin Farklı Coğrafik Bölgelerinden Toplanan Propolis Örneklerinin Aspergillus versicolor ve Penicillium aurantiogriseum’e Karşı Antifungal Aktiviteleri (İngilizce)

Year 2013, Volume: 38 Issue: 3, 135 - 142, 01.06.2013

Ayhan Temiz Ayla Şener Mumcu Aslı Özkök Tüylü Kadriye Sorkun Bekir Salih

Abstract

Bu çalışmanın amacı, Türkiye’nin çeşitli coğrafik bölgelerinden toplanan 10 propolis örneğinin mikotoksin üreticisi olan, gıda kaynaklı iki küf (Aspergillus versicolor ve Penicillium aurantiogriseum) suşuna karşı antifungal aktivitelerini araştırmaktır. Propolis örneklerinin etil alkol ekstraktlarının (EEP) kimyasal kompozisyonları kütle spektorometresine birleştirilmiş gaz kromatografisi ile belirlenmiştir. EEP örneklerinin antifungal aktiviteleri %1, %5 ve %10 olacak şekilde üç farklı konsantrasyonda test edilmiştir. EEP örneklerinin antifungal aktiviteleri, incelenen küf suşunun Patates Dekstroz Agar besiyeri yüzeyindeki misel gelişiminde meydana gelen inhibisyonun yüzdesi şeklinde belirlenmiştir. EEP örneklerinin ana bileşenleri flavonoitler, aromatik alkoller, aromatik asit esterler, alifatik asit esterler, aromatik asitler, alkoller ve aromatik hidrokarbonlar olarak tespit edilmiştir. EEP örneklerinin tümü %10 konsantrasyonda her iki küf suşunun misel gelişimi üzerinde %100 inhibisyon sağlamıştır. EEP örneklerinin iki küf suşu üzerindeki antifungal etkisi %1 ve %5 konsantrasyonlarında değişkenlik göstermiştir. Araştırma sonuçları, P. aurantiogriseum suşunun misel gelişimi üzerinde inhibisyon etkisi yaratması için A. versicolor’a kıyasla daha yüksek bir EEP konsantrasyonuna gereksinim olduğuna işaret etmektedir. Küf suşları üzerinde etkin olan propolis örneklerinin flavonoit yüzdesi diğer bileşenlerin yüzdesinden oldukça daha yüksektir. Bu sonuçlar, propolis örneklerinin EEP konsantrasyonuna ve kimyasal kompozisyonuna bağlı olarak iki küf suşu üzerinde de belirgin kuvvette bir antifungal etki yarattığına işaret etmektedir.

Keywords

Propolis, antifungal aktivite, gıda kaynaklı küfler, Aspergillus versicolor, Penicillium aurantiogriseum

Antifungal Activity of Propolis Samples Collected from Different Geographical Regions of Turkey against Two Food-Related Molds, Aspergillus versicolor and Penicillium aurantiogriseum (in English)

Abstract

The aim of this study was to investigate antifungal activities of 10 propolis samples collected from various geographical regions of Turkey against 2 important food-related mycotoxin producer molds, Aspergillus versicolor and Penicillium aurantiogriseum. Chemical compositions of ethyl alcohol extracts of the propolis (EEP) samples were determined by gas chromatography coupled to mass spectrometry. Antifungal activities of EEP samples were tested at 3 different concentrations of 1%, 5% and 10% (v/v) by determination percentage of mycelial growth inhibition of the mold strains on Potato Dextrose Agar. Main organic components of EEP samples were flavonoids, aromatic alcohols, aromatic acid esters, aliphatic acid esters, aromatic acids, alcohols and aromatic hydrocarbons. All EEP samples at 10% concentration showed 100% inhibition on mycelial growth of both mold strains. Antifungal activities of EEP samples against the mold strains were variable at 1% and 5% concentrations. The results indicated that a higher EEP concentration was necessary for the inhibitory effect on mycelial growth of P. auantiogriseum comparing with A. versicolor. Percentage of flavonoids in the effective propolis samples was considerably higher than the others. These results indicated that propolis samples had a marked antifungal action against both mold strains depending on EEP concentration and chemical composition.

Keywords

Propolis, antifungal activity, food related molds, Aspergillus versicolor, Penicillium aurantiogriseum

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