In this study, firstly the specificity of Dynabeads® anti‑Salmonella has been examined and then (immunomagnetic separation) IMS method has been investigated in order to isolate Salmonella spp. from 50 food samples assumed that having the contamination of Salmonella risk in parallel with the traditional cultural method that is ISO 6579 reference method. Also Salmonella enteritidis, anti‑Salmonella beads and the S. enteritidis +Dynabeads® anti‑Salmonella complexes have been visualized by atomic force microscope (AFM). As a result, IMS method enabled the detection of 14 Salmonella positive samples from the 50 naturally contaminated food samples examined whereas ISO 6579 reference method enabled 12 Salmonella positive samples. After applying statistical analysis to these data, this IMS method showed no difference in performance with the ISO 6579 reference method. But IMS method was found as a rapid and a highly sensitive method than the ISO 6579 reference method.
Anonymous. 2000o. bioMerieux API HIS—Package Insert, France.
Binnig G. Quate CF and Gerber C. 1986. Atomic force microscope. Phys Rev Lett. 56: 930-933.
' Bohra LK. 2000. Evaluation of 5' nuclease based assays for the detection of Escherichia coll O1 57:H7 and Salmonella Uphi
murium lrom food. cattle feces and enrichment broth systems. Kansas State University. Thesis of PhD, 2-13, USA. Bowen WR. Hilal N, Lovitt RW and Wright CJ. 1999. Atomic force microscopy. In'Enoylopeolıa of Food Microbiology. Volu
me 2. RK Robinson (chief ed), pp. 1418-1425. Academic Press. Great Britain. Cox J. 1999. Salmonellallntroduction. ln Encylopedia of Food Microbiology, Volume 2. RK Robinson (chief ed), pp. 1929
' 1937, Academic Press. Great Britain.
Cudjoe KS. Krone R and Olsen NN. 1994a. IMS: a new selective technique lor detection of Salmonella in foods. Int J Food
Microbiol. 23: 159-165. Cudjoe KS, Hagtvedt T and Dainty R. 1995. lmmunomagnetic separation of Salmonellafrom foods and their detection using
immunomagnetic particle (IMF)-ELISA. ını J Food Mlcrobiol. 27: 11-25. De Boer E and Beumer RR. 1999. Methodology for detection and typing of loodborne microorganisms. Int J Food Microbiol, 50: 119-130. Fratamico PM and Crawford CG. 1999. Detection by commercial particle-based assays. In Encylopedla of Food Mic
robiology. Volume 2. RK Robinson (chief ed). pp. 655-661. Academic Press. Great Britain. Mansfield LP and Forsythe SJ. 1993. Immunomagnetic separation as an alternative to enrichment broths for Salmonella
detection. Lett Appi Microbiol, 16: 122-125. Mansfield LP and Forsythe SJ. 2000. Detection ol salmonellae in food. Rev Med Microbiol, 11 (1): 37-46. Molla B, Kleer J and Sinell H.-J. 1994. Detection of Salmonella in foods by immunomagnetic separation. Arch Lebensrnit—
telhyg. 45 (5): 110-113. Safan‘k I and Safarikcva M. 1999. Review: Use of magnetic techniques for the isolation of cells. J Chromatogr B. 722: 33-53. Tan E. 2001. Atomik kuwet mikroskobu. TMMOB Fizik Mühendisleri Odası (FMG) Bülteni,. Haziran: 20—26.
İmmunomanyetik Ayırma Yöntem ile Gıdalardan Salmonella spp. İzolasyonu
Bu çalışmada ilk olarak, anti‑Salmonella küreciklerinin özgün1üğü incelenmiş ve ardından Salmonella kontaminasyonu açısından riskli olarak değerlendirilen 50 adet gıda örneğinden Salmonella spp. izolasyonu amacı ile, immunomanyetik ayırma (IMA) yöntemi ve geleneksel kültürel bir metot olan ISO 6579 referans metodu karşılaştırmalı olarak araştırılmıştır. Ayrıca Salmonellaenteritidis,anti‑Salmonellakürecikleri ve S. enteritidis +anti‑Salmonella kompleksleri, atomik kuvvet mikroskobu (AKM) kullanılarak görüntülenmiştir. Sonuç olarak; IMA yöntemi, doğal olarak kontamine olmuş 50 adet gıda öreğinin 14'ününSalmonella ile kontamine olduğunu belirleyebilmişken, ISO 6579 referans metodu 12 adedini belirleyebilmiştir. Bu verilere istatistiksel analiz uygulandıktan sonra, IMA yöntemi ile ISO 6579 referans metodu arasında önemli fark olmadığı belirlenmiştir. Ancak IMA yönteminin, ISO 6579 referans metoduna oranla hızlı ve yüksek duyarlıkta olduğu saptanmıştır.
Anonymous. 2000o. bioMerieux API HIS—Package Insert, France.
Binnig G. Quate CF and Gerber C. 1986. Atomic force microscope. Phys Rev Lett. 56: 930-933.
' Bohra LK. 2000. Evaluation of 5' nuclease based assays for the detection of Escherichia coll O1 57:H7 and Salmonella Uphi
murium lrom food. cattle feces and enrichment broth systems. Kansas State University. Thesis of PhD, 2-13, USA. Bowen WR. Hilal N, Lovitt RW and Wright CJ. 1999. Atomic force microscopy. In'Enoylopeolıa of Food Microbiology. Volu
me 2. RK Robinson (chief ed), pp. 1418-1425. Academic Press. Great Britain. Cox J. 1999. Salmonellallntroduction. ln Encylopedia of Food Microbiology, Volume 2. RK Robinson (chief ed), pp. 1929
' 1937, Academic Press. Great Britain.
Cudjoe KS. Krone R and Olsen NN. 1994a. IMS: a new selective technique lor detection of Salmonella in foods. Int J Food
Microbiol. 23: 159-165. Cudjoe KS, Hagtvedt T and Dainty R. 1995. lmmunomagnetic separation of Salmonellafrom foods and their detection using
immunomagnetic particle (IMF)-ELISA. ını J Food Mlcrobiol. 27: 11-25. De Boer E and Beumer RR. 1999. Methodology for detection and typing of loodborne microorganisms. Int J Food Microbiol, 50: 119-130. Fratamico PM and Crawford CG. 1999. Detection by commercial particle-based assays. In Encylopedla of Food Mic
robiology. Volume 2. RK Robinson (chief ed). pp. 655-661. Academic Press. Great Britain. Mansfield LP and Forsythe SJ. 1993. Immunomagnetic separation as an alternative to enrichment broths for Salmonella
detection. Lett Appi Microbiol, 16: 122-125. Mansfield LP and Forsythe SJ. 2000. Detection ol salmonellae in food. Rev Med Microbiol, 11 (1): 37-46. Molla B, Kleer J and Sinell H.-J. 1994. Detection of Salmonella in foods by immunomagnetic separation. Arch Lebensrnit—
telhyg. 45 (5): 110-113. Safan‘k I and Safarikcva M. 1999. Review: Use of magnetic techniques for the isolation of cells. J Chromatogr B. 722: 33-53. Tan E. 2001. Atomik kuwet mikroskobu. TMMOB Fizik Mühendisleri Odası (FMG) Bülteni,. Haziran: 20—26.
Mercanoğlu, B. ., & Aytaç, S. A. . (2005). İmmunomanyetik Ayırma Yöntem ile Gıdalardan Salmonella spp. İzolasyonu. Gıda, 30(5).
AMA
Mercanoğlu B, Aytaç SA. İmmunomanyetik Ayırma Yöntem ile Gıdalardan Salmonella spp. İzolasyonu. The Journal of Food. October 2005;30(5).
Chicago
Mercanoğlu, Birce, and S. Aykut Aytaç. “İmmunomanyetik Ayırma Yöntem Ile Gıdalardan Salmonella Spp. İzolasyonu”. Gıda 30, no. 5 (October 2005).
EndNote
Mercanoğlu B, Aytaç SA (October 1, 2005) İmmunomanyetik Ayırma Yöntem ile Gıdalardan Salmonella spp. İzolasyonu. Gıda 30 5
IEEE
B. . Mercanoğlu and S. A. . Aytaç, “İmmunomanyetik Ayırma Yöntem ile Gıdalardan Salmonella spp. İzolasyonu”, The Journal of Food, vol. 30, no. 5, 2005.
ISNAD
Mercanoğlu, Birce - Aytaç, S. Aykut. “İmmunomanyetik Ayırma Yöntem Ile Gıdalardan Salmonella Spp. İzolasyonu”. Gıda 30/5 (October 2005).
JAMA
Mercanoğlu B, Aytaç SA. İmmunomanyetik Ayırma Yöntem ile Gıdalardan Salmonella spp. İzolasyonu. The Journal of Food. 2005;30.
MLA
Mercanoğlu, Birce and S. Aykut Aytaç. “İmmunomanyetik Ayırma Yöntem Ile Gıdalardan Salmonella Spp. İzolasyonu”. Gıda, vol. 30, no. 5, 2005.
Vancouver
Mercanoğlu B, Aytaç SA. İmmunomanyetik Ayırma Yöntem ile Gıdalardan Salmonella spp. İzolasyonu. The Journal of Food. 2005;30(5).