Abstract
In this study, coffees with different grinding degrees, including very fine (47.13-54.64 µm), fine (89.25-119.88 µm) and fine medium (248.77-312.23 µm), representing the dimensions of Turkish coffee, were stored at 5, 25 and 45 °C for 28 days. The changes in chlorogenic acid content, caffeine content and fatty acid composition were investigated during storage period (0th, 14th, 28th day). Chlorogenic acid and caffeine, which are the components responsible for the bioactive properties of coffee, varied between 1.05-1.38 g/100g DM and 1.49-1.80 g/100g DM, respectively, depending on the storage conditions. Linoleic acid (41.86-42.89%) and palmitic acid (33.23-36.36%) were determined as the main fatty acids in coffee samples, as well as oleic acid (10.59-11.47%), stearic acid (7.38-8.48%) and trace amounts of myristic acid, margaric acid, elaidic acid, cis-11 eicosenoic acid, arachidic acid and behenic acids were also detected. It was observed that the difference in particle size and storage temperature of the coffee samples did not cause a significant change on the fatty acid composition ratios, but there were significant decreases in their amounts during the storage period.