AN IN VITRO ASSESSMENT OF THE BIOACTIVE, CYTOTOXIC, AND ANTIDIABETIC POTENTIAL OF DIFFERENT PARTS OF TUMBLEWEED (GUNDELIA TOURNEFORTII L.)

Year 2025, Volume: 50 Issue: 1, 28 - 41

Gözde Kutlu , Kubra Feyza Erol

https://doi.org/10.15237/gida.GD24096

Abstract

This study aimed to investigate the physicochemical properties, bioactive compounds, cytotoxicity, and antidiabetic potential of the tumbleweed plant’s roots (TR), stems & leaves (TSL), and flowers (TF). Results indicated TF as the richest part in bioactive compounds and antioxidant capacity, with the highest crude protein (13.91%), crude oil (15.50%), total phenolic content (214.64 mg GAE/g), total monomeric anthocyanin content (1132.96 mg/g), ABTS (25.48 mg TE/g), CUPRAC (203.92 mg TE/g), and FRAP (32.63 µmol Fe²⁺E/g) activities. Mineral contents varied, with Mg, K, and Ca present in significant amounts across all parts. TF also showed the highest α-glucosidase (61.99%) and α-amylase (54.92%) inhibition, suggesting strong antidiabetic potential. Cytotoxicity was weak across samples, with IC50 values ranging from 1049.76 to 1641.08 μg/ mL against HEK-293 and Caco-2 cells. These findings highlight TF as the most bioactive and nutritionally rich part of the tumbleweed plant.

Keywords

Gundelia tournefortii L., bioactive properties, antidiabetic potential, antiproliferative characteristics, mineral content.

KENGER (GUNDELIA TOURNEFORTII L.) BİTKİSİNİN FARKLI KISIMLARININ BİYOAKTİF, SİTOTOKSİK VE ANTİDİYABETİK POTANSİYELİNİN IN VITRO DEĞERLENDİRİLMESİ

Abstract

Bu çalışma, kenger bitkisinin kökleri (TR), gövde ve yaprakları (TSL) ile çiçeklerinin (TF) fizikokimyasal özelliklerini, biyoaktif bileşiklerini, sitotoksisite ve antidiabetik potansiyelini incelemeyi amaçlamıştır. Sonuçlar, en yüksek ham protein (%13.91), ham yağ (%15.50), toplam fenolik içeriği (214.64 mg GAE/g), toplam monomerik antosiyanin içeriği (1132.96 mg/g), ABTS (25.48 mg TE/g), CUPRAC (203.92 mg TE/g) ve FRAP (32.63 µmol Fe²⁺E/g) aktiviteleri açısından en zengin kısmının TF olduğunu göstermiştir. Magnezyum, potasyum ve kalsiyum gibi mineraller tüm bitki kısımlarında belirgin miktarda bulunmuştur. TF, aynı zamanda en yüksek α-glukozidaz (%61.99) ve α-amilaz (%54.92) inhibisyonu göstererek güçlü antidiabetik potansiyele işaret etmiştir. Tüm örneklerde sitotoksisite zayıf bulunmuş olup HEK-293 ve Caco-2 hücrelerine karşı IC50 değerleri 1049.76-1641.08 μg/mL arasında değişmiştir. Bu bulgular, kenger bitkisinin biyoaktif ve besin öğeleri açısından en zengin kısmının TF olduğunu göstermektedir.

Keywords

Gundelia tournefortii L. bitkisi, biyoaktif özellikler, antidiyabetik potansiyel, antiproliferatif özellikler, mineral içeriği.

Ethical Statement

Yoktur

Supporting Institution

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Thanks

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References

• Ağçam, E., Akyıldız, A. (2015). Siyah havuç posasından antosiyaninlerin ekstraksiyonuna farklı çözgen ve asit konsantrasyonlarının etkileri. GIDA, 40(3), 149-156.
• Alam, M. K., Rana, Z. H., Islam, S. N., Akhtaruzzaman, M. (2020). Comparative assessment of nutritional composition, polyphenol profile, antidiabetic and antioxidative properties of selected edible wild plant species of Bangladesh. Food chemistry, 320, 126646.
• Anonymous Turkish Food Codex Regulation on Food Labelling and Informing Consumers, Issue: 29960 (2017).
• Asghari, J., Bagheri, T., Shakeri, A. (2015). Investigation of caffeeic and chlorogenic acid, essential oil and fatty acid of Gundelia tournefortii L. fur Arznei- Gewurzpflanzen, 20, 122–126.
• Coruh, N., Celep, A. S., Özgökçe, F., İşcan, M. (2007). Antioxidant capacities of Gundelia tournefortii L. extracts and inhibition on glutathione-S-transferase activity. Food Chemistry, 100(3), 1249-1253.
• Dalar, A., Zengin, G., Mukemre, M., Bengu, A. S., İşler, S. (2019). Gundelia rosea seed: Evaluation of biopharmaceutical potential and bioactive composition. South African Journal of Botany, 125, 505-510.
• Demirkan, E. N., Akyürek, Ş. N., Bayraktar, D., Kutlu, G., Törnük, F (2024). Potential use of hazelnut (Corylus avellana L.) shell powder in muffin production by partial substitution of wheat flour: Color, bioactive, textural, and sensory properties. European Food Science and Engineering, 5(1), 1-7.
• Diab, T. A., Donia, T., Saad-Allah, K. M. (2021). Characterization, antioxidant, and cytotoxic effects of some Egyptian wild plant extracts. Beni-Suef University Journal of Basic and Applied Sciences, 10, 1-13.
• Ereifej, K. I., Feng, H., Rababah, T., Almajwal, A., Alu’datt, M., Gammoh, S. I., Oweis, L. I. (2015). Chemical composition, phenolics, anthocyanins concentration and antioxidant activity of ten wild edible plants. Food and Nutrition Sciences, 6(7), 581-590.
• Erol, K. F., Kutlu, G. (2024). Assessment of antimicrobial, anti-diabetic, anti-Alzheimer and anti-cancer properties, along with bioaccessibility and bioactive potential of Polygonum cognatum Meissn extracts from various cities in Türkiye. Food Bioscience, 105669.
• Erol, K. F., Kutlu, G., Tornuk, F., Guzel, M., Donmez, I. E. (2023). Determination of antioxidant, anticancer, antidiabetic and antimicrobial activities of Turkish red pine (Pinus brutia Ten.) bark ultrasound-assisted extract as a functional food additive. Acta Alimentaria, 52(1), 102-112.
• Erol, K.F., Kutlu, G., Oktem-Olgun, E., Tornuk, F. (2024). A sustainable innovation: Functionalization of pasta with methanol extract of Turkish red pine (Pinus brutia Ten.) barks. Waste & Biomass Valorization, 1-12.
• Haghi, G., Hatami, A., Arshi, R. (2011). Distribution of caffeic acid derivatives in Gundelia tournefortii L. Food chemistry, 124(3), 1029-1035.
• Heywood, V. H. (2011). Ethnopharmacology, food production, nutrition and biodiversity conservation: towards a sustainable future for indigenous peoples. Journal of ethnopharmacology, 137(1), 1-15.
• Kadan, S., Sasson, Y., Saad, B., Zaid, H. (2018). Gundelia tournefortii antidiabetic efficacy: Chemical composition and GLUT4 translocation. Evidence‐Based Complementary and Alternative Medicine, 2018(1), 8294320.
• Karakas, F. P., Turker, A. U., Karakas, A., Mshvildadze, V., Pichette, A., Legault, J. (2017). In vitro cytotoxic, antibacterial, anti-inflammatory and antioxidant activities and phenolic content in wild-grown flowers of common daisy—A medicinal plant. Journal of herbal medicine, 8, 31-39.
• Kayacan, S., Karasu, S., Akman, P. K., Goktas, H., Doymaz, I., Sagdic, O. (2020). Effect of different drying methods on total bioactive compounds, phenolic profile, in vitro bioaccessibility of phenolic and HMF formation of persimmon. Lwt, 118, 108830.
• Keskin, M., Kaya, G., Keskin, Ş. (2021). Gundelia Tournefortii L.(Kenger): Determination of in vitro Antidiabetic Activities. Progress in Nutrition, 23(4).
• Kutlu, G. (2015). Determination of physicochemical, compositional, conformational and rheological properties of gum produced from sage seed (Salvia sclarea) at optimum conditions, MsC thesis, Yildiz Technical University, İstanbul, Türkiye (In Turkish).
• Kutlu, G. (2024). Valorization of various nut residues grown in Turkiye: Antioxidant, anticholinesterase, antidiabetic, and cytotoxic activities. Food Science & Nutrition.
• Kutlu, G., Yılmaz, S., Karabulut, A. E. (2024a) Development of a new vegan muffin formulation: Assessing its quality and sensory characteristics. European Food Science and Engineering, 5(1), 26-34.
• Kutlu, G., Özsüer, E., Madenlioğlu, M., Eroğlu, G., Akbaş, İ. (2024b). Teff: Its applicability in various food uses and potential as a key component in gluten-free food formulations—An overview of existing literature research, European Food Science and Engineering, 5 (2), 30-54.
• Matthäus, B., Özcan, M. M. (2011). Chemical evaluation of flower bud and oils of tumbleweed (Gundelia tourneforti L.) as a new potential nutrition sources. Journal of food biochemistry, 35(4), 1257-1266.
• Mechchate, H., Es-Safi, I., Louba, A., Alqahtani, A. S., Nasr, F. A., Noman, O. M., Farooq, M., Alharbi, A. S., Alqahtani, A., Bari, A., Bekkari, H., Bousta, D. (2021). In vitro alpha-amylase and alpha-glucosidase inhibitory activity and in vivo antidiabetic activity of Withania frutescens L. Foliar extract. Molecules, 26(2), 293.
• Mehrzadeh, M., Ziayeezadeh, F., Pasdaran, A., Kozuharova, E., Goyal, R., Hamedi, A. (2024). A Review of the Ethnobotany, Biological Activity, and Phytochemistry of the Plants in the Gundelia Genus. Chemistry & Biodiversity, 21(3), e202301932.
• Özaltun, B., Daştan, T. (2019). Evaluation of antimicrobial activities and in vitro cytotoxic activities of Gundelia tournefortii L. plant extracts. Medical Journal of Süleyman Demirel University, 26(4), 436-442.
• Rana, Z. H., Alam, M. K., Akhtaruzzaman, M. (2019). Nutritional composition, total phenolic content, antioxidant and α-amylase inhibitory activities of different fractions of selected wild edible plants. Antioxidants, 8(7), 203.
• Saraç, H., Demirbaș, A., Daștan, S. D., Ataș, M., Çevik, Ö., Eruygur, N. (2019). Evaluation of nutrients and biological activities of Kenger (Gundellia tournefortii L.) seeds cultivated in Sivas province. Turkish Journal of Agriculture - Food Science and Technology, 7(sp2), 52-58.
• Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., Latha, L. Y. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African journal of traditional, complementary and alternative medicines, 8(1).
• Tarhan, A., Firat, M., Topal, G. (2023). Comprehensive study of all Gundelia L. taxa exists in the globe: An insight on LC-MS/MS based phytochemical investigation and bioactivity potential of 22 species. Biochemical Systematics and Ecology, 109, 104672.
• Yasar, B., Kutlu, G., Tornuk, F. (2022). Edible flowers as sources of bioactive compounds: Determination of phenolic extraction conditions. International Journal of Gastronomy and Food Science, 30, 100618.