Farklı dozlarda Lactobacillus buchneri ilavesinin yüksek nemli dane mısırın silolanması üzerine etkisi

Year 2023, Issue: 30, 1 - 9, 21.07.2023

Önder Canbolat

https://doi.org/10.56833/bursagida.1279059

Abstract

Amaç: Bu çalışma, farklı dozlarda Lactobacillus buchneri (LB) ilavesinin yüksek nemli dane mısırın silolanması üzerine etkisini saptamayı amaçlamıştır.

Materyal ve yöntem: Araştırmanın yem materyalini yaklaşık %70 kuru madde (KM)’de hasat edilen yüksek nemli dane mısır (YNDM) oluşturmuştur. Yüksek nemli dane mısır 0 (kontrol), 104, 105, 106 ve 107 log10 koloni oluşturan birim (kob) g-1 taze materyal (TM) oranında Lactobacillus buchneri (LB) ilave edilerek 5 grupta silolanmıştır. Her bir deneme grubu 3 tekerrür olarak 1,5 L’lik özel cam kavanozlara silolanmıştır. Silajlar 45 gün sonra açılmış ve besin madde bileşenleri saptanmıştır. Ayrıca silajların fermantasyon, aerobik stabilite, in vitro gaz üretimi ve sindirilebilirlik özellikleri saptanmıştır.

Bulgular ve sonuç: Yüksek nemli dane mısıra LB ilavesi silajların laktik asit (LA), propiyonik asit (PA) ve asetik asit (AA) ile laktik asit bakteri sayısını (LAB) önemli düzeyde artırmıştır. Buna karşın silajlara LB ilavesi pH, bütirik asit (BA), amonyak azotu (NH3N), maya ve küf miktarlarını düşürmüştür (p<0,05). Yüksek nemli dane mısıra LB ilavesi silajların in vitro gaz üretimini, metabolik enerjisini (ME) ve organik madde sindirimini (OMS) olumlu yönde etkilemiştir. Ayrıca silajların aerobik stabilitelerini geliştirmiştir. Yüksek nemli dane mısır silajları üzerinde en etkili LB dozu 107 log10 kob g-1 TM olmuştur.

Keywords

yüksek nemli dane mısır silajı, laktik asit bakterisi, silaj fermantasyonu, in vitro gaz üretimi, aerobik stabilite

Effect of adding different doses of Lactobacillus buchneri on silage of high moisture corn grain

Abstract

Objective: This study aimed to determine the effect of adding different doses of Lactobacillus buchneri on high moisture corn grain silage.

Material and method: The feed material for the study consisted of high moisture corn grain (HMCG) harvested at approximately 70% dry matter (DM). High moisture grain corn was ensiled by adding Lactobacillus buchneri (LB) at a rate of 104, 105, 106 and 107 log10 colony-forming unit: (cfu) g-1 of fresh material (FM). Silage groups were siloed into 1.5 L glass jars as 3 replications each. Silages were opened after 45 days and nutrient compositions were determined. In addition, the fermentation, aerobic stability, in vitro gas production and digestibility properties of silages were determined.

Results and conclusion: The addition of LB to high moisture corn grain significantly increased the lactic acid (LA), propionic acid (PA) and acetic acid (AA) and lactic acid bacteria (LAB) contents of silages. On the other hand, the addition of LB to silages decreased the pH, butyric acid (BA), ammonia nitrogen (NH3N), yeast and mold contents (p<0.05). Addition of LB to high moisture corn grain positively affected in vitro gas production, metabolic energy (ME), and organic matter digestion (OMS) of silages. It also improved the aerobic stability of silages. The most effective dose of LB on high moisture corn grain silages was 107 log10 cfu g-1 FM.

Keywords

high moisture corn grain silage, lactic acid bacteria, silage fermentation, in vitro gas production, aerobic stability

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