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Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province

Year 2020, Volume: 37 Issue: 1, 53 - 59, 30.04.2020
https://doi.org/10.13002/jafag4685

Abstract

In this research, it was aimed to determine existence of some viruses, causes problem, by serological and molecular techniques in potato production in Tokat province was carried out by serological and molecular tools. For this purpose, the survey was conducted to collect samples in potato production areas in Başçiftlik, Niksar, and Artova districts of Tokat province. DAS-ELISA test was performed to determine the prevalence of Potato virus Y (PVY), Potato leaf roll virus (PLRV) and Alfalfa mosaic virus (AMV). All samples were also tested by RT-PCR using specific primers to confirm the results DAS-ELISA. The results revealed that 54 % of samples were infected with PVY, PLRV, and AMV. PVY was the most common virus in the survey areas with the ratio of 50 %, followed by PLRV (5.5%) and AMV (1.38%) in all tested samples, respectively. Mixed infections also were detected within collected plants. The mixed infections PVY+PLRV was identified in four samples while each of mixed infection with PLRV+AMV, PVY+AMV and PVY+PLRV was represented with one isolate. RT-PCR amplified the expected PCR products for viruses. The best of our knowledge, AMV was first determined on potato in Tokat province.

References

  • Abou-Jawdah Y, Sobh H, El-Zammar S, Fayyad A, Lecoq H ( 2000). Incidence and management of virus diseases of cucurbits in Lebanon. Crop Protection, 19:217-224.
  • Arlı-Sökmen M, Ayan AK, Şevik MA (2005). Trabzon ve Bayburt illerinde tohumluk patates (Solanum tuberosum L.) yumrularında belirlenen virüsler. OMÜ Ziraat Fakültesi Dergisi. 20 (3), 23–26.
  • Astruc N, Marcos JF, Macquaıre G, Candresse T, Pallás V (1996). Studies on the diagnosis of hop stunt viroid in fruit trees: identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents. European Journal of Plant Pathology, 102: 837-846.
  • Bol JF (2003). Alfalfa mosaic virus: coat protein-dependent initiation ofinfection. Mol Plant Pathol 4:1–8
  • Bora T ,Karaca İ (1970). Kültür Bitkilerinde Hastalığın ve Zararın Ölçülmesi. E.Ü. Ziraat Fakültesi YardımcıDers Kitabı, Yayın No:167, pp:43
  • Bostan H, Açıkgöz S (2000). Determination of PVX and PVS symptoms on some test plants and identification of these viruses using dsRNA analysis. The Journal of Turkish Phytopathology, 29(1): 41:47.
  • Bostan H, Haliloğlu K (2004). Distribution of PLRV, PVS, PVX, and PVY (PVYN, PVY0 and PVYc) in the seed potato tubers in Turkey. Pakistan Journal of Biological Sciences, 7(7): 1140-1143.
  • Brunt AA, Loebenstein G (2001). The Main Viruses Infecting Potato Crops, In: Virus and Virus-like Diseases of Potatoes and Production of Seed- potatoes. Eds: Loebenstein G, Berger PH, Brunt AA and Lawson RH. Kluwer Academic Publishers, Dordrecht, The Netherlands: 65-134.
  • Burrows ME, Zitter TA (2005). Virus Problems of Potatoes. Department of Plant Pathology, USDA-ARS, Cornell University, NY 14853 April, Ithaca.
  • Buzkan N, Arpacı BB, Görsoy G, Zencirkıran M, Moury Benoit (2015). Genetic variability of Potato virus Y (PVY) and Tobacco etch virus (TEV) from naturally infected pepper fields in the Hatay region of Turkey.Archives of Phytopathology and Plant Protection, Taylor and Francis, 48 (7), pp.588-600.
  • Clark MF, Adams AN (1977). Charecteristic of microplatemethod of Enzyme-linked immuno sorbent assay for detection of plant viruses. J. Gen. Virol., 34: 475-483.
  • Çarpar H, Sertkaya G (2016). Hatay İli Patates Üretiminde Önemli Bazı Viral Sorunların Belirlenmesi. Nevşehir Bilim ve Teknoloji Dergisi TARGİD Özel Sayı 135-143
  • Çıtır A, (1982). Erzurum ve çevresinde tohumluk patateslerdeki virus hastalıkları ve bunların tanılanması üzerinde bazı araştırmalar. Doğa Bilim Dergisi 6(3): 99-109.
  • Çıtır A, Tugay ME, Doğanlar M, Yılmaz G, Eraslan F, Kara K, Çağatay K (1999). Tokat ilinde yayla ve ova koşullarında tohumluk patates üretimini sınırlayan zararlılar ve hastalıklar. II. Ulusal Patates Kongresi. 28-30 Haziran, Erzurum. 185-190.
  • FAO 2020. http://www.fao.org/faostat. 13-03-2020
  • Gümüş M, Erkan S (1998). Ayvalık ve Altınova yörelerinde üretilen patates çeşitlerinin yumrularında bulunan virüslerin belirlenmesi üzerinde araştırmalar. VIII. Türkiye Fitopatoloji Kongresi Bildirileri, Ankara, 348–350.
  • Guner U, Yorgancı U (2006). Plant viruses detected in the potato growing areas in Niğde and Nevşehir Provincesr, Plant Production Bulletion, 46 (1-4), 35-49.
  • Hameed A, Iqbal Z, Asad S, Mansoor S (2014). Detection of Multiple Potato Viruses in the Field Suggests Synergistic Interactions among Potato Viruses in Pakistan. Plant Pathology Journal 30(4) : 407-415
  • He Z, Larkin RP, Honeycutt W (2012). Sustainable potato production. In: global case studies. 1st ed. Springer, Dordrecht, Heidelberg, New York, London
  • Hooker W J (1986). Compendium of Potato Diseases. American Phytopathologycal Society Press, St. Paul, Minnesota, 125 PP.
  • Jeffries C, Barker H, Khurana SMP (2005). Potato viruses (and viroids) and their management. In: Potato production, improvement and post-harvest management. The Haworth’s Food Products Press, New York, USA.
  • Kutluk Yılmaz N D, Yanar Y, Kadioglu I, Cesmeli I, Erkan S (2003). Detection of Viruses in Potato Leaves, Seed Tubers and Weeds by ELISA in Tokat Province, Turkey. J. Turk. Phytopath., VoL32, No. 3, 145-156
  • Ozdemir S, Erilmez S, Paylan I. C (2011). Serological and Molecular Identification of Alfalfa mosaic alfamovirus in Potato Production Areas in Aegean Region. 410.Proceedings of the Fourth Plant Protection Congress of Turkey. 410
  • Salazar LF (2003). Potato Viruses after The XXth Century: Effects, Dissemination and Their Control. Crop Protection Department, Seminar Transcript CIP, P. O. Box: 1558, Lima 12, Peru.
  • Salazar LF (1996). Potato Viruses and Their Control. CIP, Lima. 214 pp.
  • Sertkaya E, Sertkaya G (2005). Aphid Transmission of Two Important Potato Viruses, PVY and PLRV by Myzus persicae (Sulz.) and Aphis gossypii (Glov.) in Hatay Province of Turkey. Pakistan Journal of Biological Sciences. 8(9): 1242-1246.
  • Sertkaya G, Çalışkan ME (2009). Hatay ilinde yetiştirilen patateslerde yumrularda simptom oluşturan önemli virüslerin serolojik ve biyolojik yöntemlerle araştırılması. Türkiye VIII. Tarla Bitkileri Kongresi, 19-22 Ekim, Hatay, Cilt II : 227-230.
  • Sertkaya G (2013). Status of Potato Production and Virus Diseases in Turkey. 15th European Association for Potato Research (EAPR) Virology Section Meeting. (28-31 May, Antalya, Turkey)
  • Sertkaya G, Üremiş I, Sertkaya E, Kaya K, Çalışkan ME (2009). Amik Ovasında patates alanlarındaki yabancı ot türlerinin yoğunlukları ile bazı önemli patates virüsleri ve vektörleri yönünden araştırılması. Türkiye VIII. Tarla Bitkileri Kongresi, 19-22 Ekim, Hatay, Cilt I : 143-145.
  • Sertkaya G, Çarpar H, Sertkaya E (2017). Detection of Alfalfa Mosaic Virus (AMV) in Potato Production Areas in Hatay Province of Turkey. Iğdır University Journal of the Institute of science and Technolog. 7(1): 23-29
  • Singh RP, Kurz J, Boiteau G (1995). Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. J. Virol. Methods, 55: 133-143.
  • Singh RP, Singh M (1998). Specific dedection of Potato virus A in dormant tubers by reverse transcription polymerase chain reaction. Plant Diseases, 82:230-234.
  • Stevenson W R, Loria R, Franc GD and Weingartner D P (2001). Compendium of Potato Diseases. Second Edittion, APS Press, 3340 Pilot Knob Road, St. Paul, Minnesota, USAWang B, MA Y, Zhang Z, Wu Z, Wu Y, Wang Q and Li M (2011). Potato viruses in China. Crop Prot. 30:1117-1123.
  • Xu H, Nie J (2006). Identification, charac-terization, and molecular detection of alfal-fa mosaic virus in potato. Phytopathology. 96(11): 1237-1242.
  • Yardımcı N; Kılıç H Çulal, Demir, Y (2015). Detection of PVY, PVX, PVS, PVA, and PLRV on Different Potato Varieties in Turkey Using DAS-ELISA. Journal of Agricultural Science and Technology. Vol. 17: 757-764
  • Yılmaz MA, Baloğlu S, Nas YZ (1990). Çukurova Bölgesi’nde yetiştirilen turfanda patateslerde patates yaprak kıvırcıklık virüsünün (PLRV) ELISA testi ile surveyi. Ç.Ü. Ziraat Fakültesi Dergisi, 5(3): 95-106.

Tokat ili patates üretim alanlarında bazı virüslerin serolojik ve moleküler yöntemlerle belirlenmesi

Year 2020, Volume: 37 Issue: 1, 53 - 59, 30.04.2020
https://doi.org/10.13002/jafag4685

Abstract

Bu araştırmada, Tokat ili patates üretim alanlarında sorun olan viral etmenlerin varlığının serolojik ve moleküler olarak belirlenmesi amaçlanmıştır. Bu amaç kapsamında, Tokat ilinde patates yetiştiriciliğinin en çok yapıldığı Merkez, Niksar, Artova, Başçiftlik ilçelerine surveyler yapılmıştır. Surveyler esnasında toplanan örnekler Patates Y virus (Potato virus Y-PVY), Patates yaprak kırılma virüsü (Potato leaf roll virus-PLRV) ve Yonca mozaik virüsü (Alfalfa mosaic virus-AMV) etmenlerinin varlığını belirlemek için öncelikle serolojik olarak DAS-ELISA ile testlenmiştir. Bütün örnekler DAS-ELISA test sonuçlarını kontrol etmek amacıyla spesifik primerler kullanılarak RT-PCR ile de testlenmiştir. Çalışmalar sonucunda, bölgeden toplanan örneklerin %54’ünde bir veya birden fazla virüs enfeksiyonu tespit edilmiştir. Survey alanlarından toplanan örneklerde en yüksek oran ile %50 PVY tespit edilirken, %5.5 oranla PLRV ve %1.38 oranında AMV enfeksiyonu tespit edilmiştir. Toplanan örneklerde düşük oranlarda karışık enfeksiyonlarda görülmüş olup 4 bitkide PVY+PLRV tespit edilmiş iken, birer bitkide PLRV+AMV, PVY+AMV ve PVY+PLRV karışık enfeksiyonları belirlenmiştir. Çalışma konusu virüslere spesifik primerlerle yapılan RT-PCR çalışmaları sonucunda beklenilen büyüklükte PCR ürünleri elde edilmiştir. Yapılan bu çalışma ile Tokat ilinde patates alanlarında AMV’ nin varlığı ilk defa tespit edilmiştir. 

References

  • Abou-Jawdah Y, Sobh H, El-Zammar S, Fayyad A, Lecoq H ( 2000). Incidence and management of virus diseases of cucurbits in Lebanon. Crop Protection, 19:217-224.
  • Arlı-Sökmen M, Ayan AK, Şevik MA (2005). Trabzon ve Bayburt illerinde tohumluk patates (Solanum tuberosum L.) yumrularında belirlenen virüsler. OMÜ Ziraat Fakültesi Dergisi. 20 (3), 23–26.
  • Astruc N, Marcos JF, Macquaıre G, Candresse T, Pallás V (1996). Studies on the diagnosis of hop stunt viroid in fruit trees: identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents. European Journal of Plant Pathology, 102: 837-846.
  • Bol JF (2003). Alfalfa mosaic virus: coat protein-dependent initiation ofinfection. Mol Plant Pathol 4:1–8
  • Bora T ,Karaca İ (1970). Kültür Bitkilerinde Hastalığın ve Zararın Ölçülmesi. E.Ü. Ziraat Fakültesi YardımcıDers Kitabı, Yayın No:167, pp:43
  • Bostan H, Açıkgöz S (2000). Determination of PVX and PVS symptoms on some test plants and identification of these viruses using dsRNA analysis. The Journal of Turkish Phytopathology, 29(1): 41:47.
  • Bostan H, Haliloğlu K (2004). Distribution of PLRV, PVS, PVX, and PVY (PVYN, PVY0 and PVYc) in the seed potato tubers in Turkey. Pakistan Journal of Biological Sciences, 7(7): 1140-1143.
  • Brunt AA, Loebenstein G (2001). The Main Viruses Infecting Potato Crops, In: Virus and Virus-like Diseases of Potatoes and Production of Seed- potatoes. Eds: Loebenstein G, Berger PH, Brunt AA and Lawson RH. Kluwer Academic Publishers, Dordrecht, The Netherlands: 65-134.
  • Burrows ME, Zitter TA (2005). Virus Problems of Potatoes. Department of Plant Pathology, USDA-ARS, Cornell University, NY 14853 April, Ithaca.
  • Buzkan N, Arpacı BB, Görsoy G, Zencirkıran M, Moury Benoit (2015). Genetic variability of Potato virus Y (PVY) and Tobacco etch virus (TEV) from naturally infected pepper fields in the Hatay region of Turkey.Archives of Phytopathology and Plant Protection, Taylor and Francis, 48 (7), pp.588-600.
  • Clark MF, Adams AN (1977). Charecteristic of microplatemethod of Enzyme-linked immuno sorbent assay for detection of plant viruses. J. Gen. Virol., 34: 475-483.
  • Çarpar H, Sertkaya G (2016). Hatay İli Patates Üretiminde Önemli Bazı Viral Sorunların Belirlenmesi. Nevşehir Bilim ve Teknoloji Dergisi TARGİD Özel Sayı 135-143
  • Çıtır A, (1982). Erzurum ve çevresinde tohumluk patateslerdeki virus hastalıkları ve bunların tanılanması üzerinde bazı araştırmalar. Doğa Bilim Dergisi 6(3): 99-109.
  • Çıtır A, Tugay ME, Doğanlar M, Yılmaz G, Eraslan F, Kara K, Çağatay K (1999). Tokat ilinde yayla ve ova koşullarında tohumluk patates üretimini sınırlayan zararlılar ve hastalıklar. II. Ulusal Patates Kongresi. 28-30 Haziran, Erzurum. 185-190.
  • FAO 2020. http://www.fao.org/faostat. 13-03-2020
  • Gümüş M, Erkan S (1998). Ayvalık ve Altınova yörelerinde üretilen patates çeşitlerinin yumrularında bulunan virüslerin belirlenmesi üzerinde araştırmalar. VIII. Türkiye Fitopatoloji Kongresi Bildirileri, Ankara, 348–350.
  • Guner U, Yorgancı U (2006). Plant viruses detected in the potato growing areas in Niğde and Nevşehir Provincesr, Plant Production Bulletion, 46 (1-4), 35-49.
  • Hameed A, Iqbal Z, Asad S, Mansoor S (2014). Detection of Multiple Potato Viruses in the Field Suggests Synergistic Interactions among Potato Viruses in Pakistan. Plant Pathology Journal 30(4) : 407-415
  • He Z, Larkin RP, Honeycutt W (2012). Sustainable potato production. In: global case studies. 1st ed. Springer, Dordrecht, Heidelberg, New York, London
  • Hooker W J (1986). Compendium of Potato Diseases. American Phytopathologycal Society Press, St. Paul, Minnesota, 125 PP.
  • Jeffries C, Barker H, Khurana SMP (2005). Potato viruses (and viroids) and their management. In: Potato production, improvement and post-harvest management. The Haworth’s Food Products Press, New York, USA.
  • Kutluk Yılmaz N D, Yanar Y, Kadioglu I, Cesmeli I, Erkan S (2003). Detection of Viruses in Potato Leaves, Seed Tubers and Weeds by ELISA in Tokat Province, Turkey. J. Turk. Phytopath., VoL32, No. 3, 145-156
  • Ozdemir S, Erilmez S, Paylan I. C (2011). Serological and Molecular Identification of Alfalfa mosaic alfamovirus in Potato Production Areas in Aegean Region. 410.Proceedings of the Fourth Plant Protection Congress of Turkey. 410
  • Salazar LF (2003). Potato Viruses after The XXth Century: Effects, Dissemination and Their Control. Crop Protection Department, Seminar Transcript CIP, P. O. Box: 1558, Lima 12, Peru.
  • Salazar LF (1996). Potato Viruses and Their Control. CIP, Lima. 214 pp.
  • Sertkaya E, Sertkaya G (2005). Aphid Transmission of Two Important Potato Viruses, PVY and PLRV by Myzus persicae (Sulz.) and Aphis gossypii (Glov.) in Hatay Province of Turkey. Pakistan Journal of Biological Sciences. 8(9): 1242-1246.
  • Sertkaya G, Çalışkan ME (2009). Hatay ilinde yetiştirilen patateslerde yumrularda simptom oluşturan önemli virüslerin serolojik ve biyolojik yöntemlerle araştırılması. Türkiye VIII. Tarla Bitkileri Kongresi, 19-22 Ekim, Hatay, Cilt II : 227-230.
  • Sertkaya G (2013). Status of Potato Production and Virus Diseases in Turkey. 15th European Association for Potato Research (EAPR) Virology Section Meeting. (28-31 May, Antalya, Turkey)
  • Sertkaya G, Üremiş I, Sertkaya E, Kaya K, Çalışkan ME (2009). Amik Ovasında patates alanlarındaki yabancı ot türlerinin yoğunlukları ile bazı önemli patates virüsleri ve vektörleri yönünden araştırılması. Türkiye VIII. Tarla Bitkileri Kongresi, 19-22 Ekim, Hatay, Cilt I : 143-145.
  • Sertkaya G, Çarpar H, Sertkaya E (2017). Detection of Alfalfa Mosaic Virus (AMV) in Potato Production Areas in Hatay Province of Turkey. Iğdır University Journal of the Institute of science and Technolog. 7(1): 23-29
  • Singh RP, Kurz J, Boiteau G (1995). Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. J. Virol. Methods, 55: 133-143.
  • Singh RP, Singh M (1998). Specific dedection of Potato virus A in dormant tubers by reverse transcription polymerase chain reaction. Plant Diseases, 82:230-234.
  • Stevenson W R, Loria R, Franc GD and Weingartner D P (2001). Compendium of Potato Diseases. Second Edittion, APS Press, 3340 Pilot Knob Road, St. Paul, Minnesota, USAWang B, MA Y, Zhang Z, Wu Z, Wu Y, Wang Q and Li M (2011). Potato viruses in China. Crop Prot. 30:1117-1123.
  • Xu H, Nie J (2006). Identification, charac-terization, and molecular detection of alfal-fa mosaic virus in potato. Phytopathology. 96(11): 1237-1242.
  • Yardımcı N; Kılıç H Çulal, Demir, Y (2015). Detection of PVY, PVX, PVS, PVA, and PLRV on Different Potato Varieties in Turkey Using DAS-ELISA. Journal of Agricultural Science and Technology. Vol. 17: 757-764
  • Yılmaz MA, Baloğlu S, Nas YZ (1990). Çukurova Bölgesi’nde yetiştirilen turfanda patateslerde patates yaprak kıvırcıklık virüsünün (PLRV) ELISA testi ile surveyi. Ç.Ü. Ziraat Fakültesi Dergisi, 5(3): 95-106.
There are 36 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Şerife Topkaya This is me

Publication Date April 30, 2020
Published in Issue Year 2020 Volume: 37 Issue: 1

Cite

APA Topkaya, Ş. (2020). Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 37(1), 53-59. https://doi.org/10.13002/jafag4685