Demir Bazlı Manyetik Nanopartiküllerin Genotoksik Etkilerinin Drosophila melanogaster’de Araştırılması (Araştırma Makalesi)

Year 2024, Volume: 5 Issue: 1, 39 - 51, 29.05.2024

Ayşen Yağmur Burgazlı , Merve Güneş , Burcin Yalcin , Ghada Tagorti , Bülent Kaya

Abstract

Manyetik nanopartiküller (MNP’ler) günümüzde nanopartiküllerin yeni bir sınıfı olarak biyosensörler, tıbbi tanı ve tedavi, manyetik rezonans görüntüleme ve daha birçok alanda sıklıkla kullanılmaya başlanmıştır. MNP’lerin kullanım alanları arasında özellikle insan üzerinde yaygın uygulama alanlarına sahip olmaları ve ayrıca potansiyel toksisiteleri hakkında literatürde bilgi eksikliği olması sebebiyle MNP’lerin kullanımı oldukça endişe uyandırıcı bir durum haline gelmiştir. Bu bağlamda MNP’lerin toksikolojik açıdan değerlendirilmeleri büyük bir önem taşımaktadır. Bu bağlamda çalışmamızda 4 farklı MNP’nin (Fe3O4 NP, NiFe2O4 NP, CoFe2O4 NP ve MnFe2O4 NP) genotoksik potansiyelleri, genotoksikoloji alanında sıklıkla kullanılan bir model organizma olan Drosophila melanogaster ile kanat somatik mutasyon ve rekombinasyon testi (SMART) yöntemi çalışılarak araştırılmıştır. Çalışmada kullanılan uygulama konsantrasyonları 1, 3, 5 ve 10 mM olarak belirlenmiştir. SMART yönteminden elde edilen sonuçlara göre; NiFe2O4 MNP’lerinin en yüksek konsantrasyonunda (10 mM) ve CoFe2O4 MNP’nin ise 3, 5 ve 10 mM’lık konsantrasyonlarında toplam klon sayısı parametresine göre istatistiksel olarak anlamlı sonuçların ortaya çıktığı ve genotoksisitenin indüklendiği tespit edilmiştir.

Keywords

Drosophila melanogaster, Manyetik nanopartiküller, SMART yöntemi, Genotoksisite

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FYL-2021-5714

Thanks

Bu çalışma Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından FYL-2021-5714 numaralı proje ile desteklenmiştir.

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