In vitro Antibacterial, Antifungal and Anti-Mycobacterium Activity of Selected Lamiaceae and Asteraceae Species

Year 2019, Volume: 1 Issue: 1, 21 - 27, 15.12.2019

Berrin Özçelik , Rachid Belhattab İlkay Erdoğan Orhan

Abstract

In this study, six extracts obtained from
various plants of Lamiaceae and
Asteraceae families were screened in
vitro against Gram negative and
Gram positive bacteria, as well as Mycobacterium
tuberculosis and two yeasts.
Acetone extracts of Origanum glandulosum, Marrubium vulgare and
Artemisia herba alba as well as ethanol extracts of Artemisia herba
alba, A. absinthium and A. fragrance were used. Activity of
the extracts were screened against Escherichia
coli, Salmonella enteritidis; Staphylococcus aureus, Enterococcus faecalis;
Candida albicans, Candida krusei and
Mycobacterium tuberculosis H37Rv
using micro-dilution method according to the Clinical and Laboratory
Standards Institute. All the extracts showed antimicrobial activity
against Gram positive and Gram negative bacteria at concentrations ranging
between 64-256 µg/mL. Anti-Candida activities of the extract developed the same
MIC values towards all the extracts tested (MIC: 128 µg/mL) whereas A. herba
alba ethanol extract was more effective against C. krusei at the
same potency as the standard fluconazole (MIC: 64 µg/mL). Anti-mycobacterium
activity was at the same MIC values of 128 µg/mL for all the extracts tested. Tested total extracts showed antimicrobial activity with higher MIC
values than standard antimicrobial agents. Fractions of extracts or isolated
metabolites could prove to be more potent against pathogenic microorganisms.

Keywords

Lamiaceae, Asteraceae, Antimicrobial activity, Antifungal activity, Mycobacteria

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