Isolation and Molecular Identification of Streptomyces tendae and its Xylanase Production Using Cost-Effective Agro‐Residues

Abstract

Due to their biotechnological importance in different industrial fields, xylanases from microorganisms have been the focus of great attention. In this regard, bacterial strain, SHB_02, one of the best xylanase producer isolated during a screening study from soil, was identified as Streptomyces tendae (GenBank accession no. OP893637) based on phenotypic characters and 16S rRNA gene sequencing. Experiments were carried out with three different agro-residues, such as corncob, wheat bran and wheat straw, for xylanase production by fermentation. The optimum parameters for producing the xylanase from Streptomyces tendae were temperature 30 oC, pH 7.5, incubation period 72 h, inoculum concentration 2%, and shaking speed 140 rpm. Among the agricultural by-products, wheat straw, 3% (w/v), has the highest xylanase as the sole carbon source. On the other hand, the best nitrogen source was determined to be yeast extract (0.25%). The xylanase was stable at pH 8 and 50 oC for at least two h. These findings suggest that a novel strain of Streptomyces tendae may produce xylanase that can be obtained inexpensively by fermentation using agro-residues.

Keywords

• Agro-Residues
• Fermentation
• Production
• Streptomyces tendae
• Xylanase activity

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