The Investigation of the Effect of Topical Vascular Endothelial Growth Factor (VEGF) Administration On Serum Oxidative Parameters in Diabetic Rats

Abstract

There is positiveeffect of exogenvascularendothelialgrowthfactor (VEGF) applicationtodiabeticwound.It is aimed to research the effects of topical VEGF administration on oxidative parameters in rat serum.In this study, 36 male wistar albino rats (weight: 200-250 g) were used. All rats get diabetic with streptozotocin. Dorsalateral excisional wounds (4 cm lengthy) were made on the two sides of medulla spinalis on the rats and they were randomly divided into 3 groups: untreated groups (n=12), chitosan treated (n=12)and chitosan+ VEGF (n=12)(7 ng/ml). In chitosan treated groups, wounds treated topically with equal amount blank chitosan gel. After this administration on the 3 th and 7 th days of wound,animals were sacrified. NOx, TBARS and RSH levels were measured spectrophotometrically in serum. Results were expressed as mean ± standart deviation. Mean differences were compared by Anova Variance Analysis(p<0,05). It can be thought that topical VEGF administration on the excisional wound of diabetic rats may have possitive effect on the recovery of oxidative damage in serum.

Keywords

• VEGF
• Diabetes mellitus
• Wound.Healing
• Oxidative.Stress
• Antioxidant

References

1. Kumar, V., Cotran, R.S., Robbins, S.L ‘‘Pankreas’’. Editor: Mitchell, J. TemelPatoloji. 5’th ed. İstanbul: W B Saunders Company-Nobel Tıp Kitabevleri Ltd şti&YüceYayınları AŞ., 569–587 (1994).
2. Guyton, A.C., Hall, J.E. 'Medical Physiology', (Çevirieditörü: Çavuşoğlu H.), W.B. Saunders Company, Philadelphia, Nobel Tıp Kitabevleri, 419–422 (2001).
3. Wild, S., Roglic, G., Gren, A., et all. ‘‘Global Prevelance of Diabetes, Estimates for the year 2000 and projection for 2030’’, Diabetes Care, 27:1047- 1053. (2004) .
4. -S.A. Eming, P. Martin, M. Tomic-Canic. Wound repair and regeneration: Mechanisms, signaling, and translation Science Translational Medicine, 6 (265-266 (2014).
5. Matthias, Schafer., Sabina, Werner ‘‘Oxidative Stress In Normal And Impaired Wound Repair’’,ETH Zurich ,Institute Of Cell Biology,Pharmicological Research ,Volume 58, Issue2,page 165-171. ., (2008)
6. Chandan, K.Sen., Sashwati, Roy). ‘‘ Redox signals in wound healing Biochimica et BiophisicaActa 1780 1348-1361.. (2008)
7. Khanna, S., Sen, C.K “Redox regulation of the VEGF signaling path and tissue vascularization : Hydrogen peroxide the comon link between physical exercise and cutaneous wound healing”, Free Radical Biology and Medicine. ;44(2): 180-192.( 2008)
8. Nissen NN, Polverini PJ, Koch AE, Volin MV, Gamelli RL, DiPietro LA. Vascular endothelial growth factor mediates angiogenic activity during the proliferative phase of wound healing. Am J Pathol. 1998;152:1445–1452.

9. T. Murohara, J.R. Horowitz, M. Silver, et alVascular endothelial growth factor/vascular permeability factor enhances vascular permeability via nitric oxide and prostacyclinCirculation, 97:99 (1998)
10. D.D. Ku, J.K. Zaleski, S. Liu, et alVascular endothelial growth factor induces EDRF-dependent relaxation in coronary arteriesAm J Physiol, 265 :286 (1993),
11. R. van der Zee, T. Murohara, Z. Luo, et al. Vascular endothelial growth factor/vascular permeability factor augments nitric oxide release from quiescent rabbit and human vascular endothelium Circulation, 95:1030 (1997),
12. .Sashwati, R., Savita, K., Kishore, N., Thomas , K. H., Chandan, K. S ‘‘Dermal wound healing is subject to redox control’’. MolTher. 13(1): 211–220. (2006 )
13. Kolluru ,G. K., Shyamal,C., Christopher, G. K., ‘‘ Endothelial Dysfunction and Diabetes: Effects on Angiogenesis, Vascular Remodeling, and WoundHealing’’,International.Journal.of.VascularMedicine (2012).
14. Kurtel H, Granger DN, Tso P, Grisham MB: Vulnerability of intestinal interstitial fluid to oxidant stress. Am J Physiol. 262: G573-578 (1992)
15. Elmann
16. Miranda KM, Espey MG, Wink DA: A rapid, simple spectrophotometric
17. a. method for simultaneous detection of nitrate and nitrite. Nitric Oxide. 5(1): 62-71 (2001).
18. Ekmekçi, P., Bostancı, S. "Wound healing",T. KlinDermatoloji, 12: 114-120(2002).
19. Galiano, R. D., Tepper, O., Pelo M. C. R,. et al., “Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells,” American Journal of Pathology164: 1935–1947( 2004).
20. Ferrara, N., Davis-Smyth, T. “ The biology of VEGF ” Endocrine Reviews ; 18:4-25. (1997)
21. Yancopoulos, G. D., Davis, S., Gale , N. W., Rudge, J. S., Wiegand, S. J., Holash, J.) “Vascular-specific growth factors and blood vessel formation”. Nature; 407:242-8. (2000)
22. Rivard, A., Silver, M., Chen, D. et al“ Rescue of diabetes-related impairment of angiogenesis by intramuscular gene therapy with adeno-VEGF”. Am J Pathol ;154:355..(1999)
23. Simoe, R., Subash, C. G., Madan, M. C. Bharat, B. A. “Oxidative.stress,inflammation,and.cancer:Howare.they.linked? ” Free RadicBiol Med; 49(11): 1603–1616. (2010)