Gram-Negative Bacteria

Abstract

Antagonistic Effects of Some Lactobacilli On Some Gram-Negative Bacteria

Abstract

Three lactobacilli strains were examined for the inhibitory activity against some gram-negative bacteria with a comparison of well diffusion and spot on lawn method. The inhibitory activity of lactobacilli under spot on lawn method showed significant clear zones. Although in spot on lawn method, lactobacilli have strongest antagonistic activity against P. aeruginosa ATCC 27853, in well diffusion method, it was the least sensitive tested bacteria. In spite of E. coli O157:H7 is inhibited secondly in well diffusion method (16mm), the value of the inhibition is lower than spot on lawn method (26mm). These results showed that spot on lawn method is better method than well diffusion method.

 

 

Key Words: Antagonism, Gram-negative bacteria, Lactic acid bacteria, Spot on lawn method, Well diffusion method

 

Keywords

• Antagonism, Gram-negative bacteria, Lactic acid bacteria, Spot on lawn method, Well diffusion method

References

1. Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T., “Bergey’s Manual of Determinative Bacteriology, 9th edition”. Williams and Wilkins, 566, (1994).
2. Daeschel, M.A., “Antibacterial substances from lactic acid bacteria for use as food preservatives”, Food Technol., 43, 164-167 (1989).
3. Klaenhammer, T.R., “Bacteriocins of lactic acid bacteria”, Biochemie, 70, 337-349 (1988).
4. Rodriguez, J.M., Sobrino, O.J., Fernandez, M.F., Hernandez, P.E., Sanz, B., “Antimicrobial activity of lactic acid bacteria isolated from Spanish dry fermented sausages”, 35th Int. Congr. Meat Sci. Technol., Copenhagen: 308-312, (1989).
5. Ross K.F., Ronson, C.W., Tagg, J.R., “Isolation and characterization of the antibiotic salivaricin A and its structural gene salA from Streptococcus salivarius 20P3”, Appl. Environ.Microb., 59(7): 2014-2021 (1993).
6. Cadirci B.H., Citak, S., “A comparison of two methods used for measuring antagonistic activity of lactic acid bacteria”, Pak. J. Nutr., 4 (4):237-241, (2005).
7. Kivanç, M., “Antagonistic action of lactic cultures toward spoilage and pathogenic microorganisms in food”, Die Nahrung, 34(3): 273-277 (1990).
8. Schillinger, U., Lucke F.K., “Antibacterial activity of Lactobacillus sake isolated from meat”, Appl. Environ. Microbiol., 55(8):1901-1906 (1989).

9. Con, A.H., Gokalp, H.Y., “Production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples”, Meat Sci., 55: 89-96, (2000).