An EPR Study of Gamma Irradiated Medicinal Plants: Cress Seeds and Mistletoe

Abstract

The EPR spectral properties of non-irradiated and gamma-irradiated dry plants, cress seeds and mistletoe, have been studied with electron paramagnetic resonance method and the differences between non-irradiated and irradiated samples have been determined within this study. It has been shown that EPR spectra of these non-irradiated samples have very weak signal. But these plants have an intense EPR resonance line after irradiation with g value of 2.0046 ±0.0005.

Keywords

• EPR
• Gamma irradiation
• Medicinal plants.

References

1. [1] Yordanov, N.D., Gancheva, V., Radicheva, M., Hristova, B., Guelev, M., Penchev, O., “Comparative identification of irradiated herbs by the methods of electron paramagnetic resonance and thermoluminescence”, Spectrochim. Acta A 54: 2413–2419 (1998).
2. [2] Yordanov, N.D., Pachova, Z., “Gamma-irradiated dry fruits An example of a wide variety of longtime dependent EPR spectra”, Spectrochimica Acta Part A 63: 891–895 (2006).
3. [3] Yordanov, N.D., Aleksieva, K., Dimitrova, A., Georgieva, L., Tzvetkova, E., “Multifrequency EPR study on freeze-dried fruits before and after X-ray irradiation”, Radiation Physics and Chemistry 75: 1069–1074 (2006).
4. [4] Raffi, J., Agnel, J.P.L., “Electron spin resonance of irradiated fruits”, Radiat. Phys. Chem. 34: 891–894 (1989).
5. [5] Raffi, J., Yordanov, N.D., Chabane, S., Douifi, L., Gancheva, V., Ivanova, S., “Identification of irradiated treatment of aromatic herbs and spices by electron paramagnetic resonance and thermoluminescence”, Spectrochim. Acta A 56: 409–416 (2000).
6. [6] Raffi, J., Belliardo, J.J., Agnel, J.P.L., “Application of ESR to identification of irradiated foodstuffs”, J. Chim. Phys. 91: 1913–1929 (1994).
7. [7] Polonia, I., Esteves, M.P., Andrade, M.E., Empis, J., “Identification of irradiated peppers by electron spin resonance, thermoluminescence and viscosity”, Radiat. Phys. Chem. 46: 757–760 (1995).
8. [8] Yordanov, N.D., Aleksieva, K., “X- and Q-band EPR studies on fine powders of irradiated plants. New approach for detection of their radiation history by using Q-band EPR spectrometry ”, Radiat. Phys. Chem. 69: 59–64 (2004).

9. [9] Su, L., Yin, J.J., Charles, D., Zhou, K., Moore, J., Yu, L., “Total phenolic contents, chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf”, Food Chemistry 100: 990–997 (2007).
10. [10] EN Protocol EN 1786, “Detection of irradiated food containing bone: analysis by electron paramagnetic resonance”, European Committee for Standardization, Brussels (1997).
11. [11] EN Protocol EN 1787, “Determination of irradiated food containing cellulose: analysis by EPR”, European Committee for Standardization, Brussels (2000).
12. [12] EN Protocol EN 13708, “Detection of irradiated food containing crystalline sugar: analysis by EPR”, European Committee for Standardization, Brussels (2001).
13. [13] Yordanov, N.D., Gancheva, V., “A new approach for extension of the identification period of irradiated cellulose containing foodstuffs by EPR spectroscopy”, Appl. Radiat. Isot. 52: 195–198 (2000).
14. [14] Yordanov, N.D., Gancheva, V., Recent development of EPR dosimetry. In: Lund, A., Shiotani, M. (Eds.), “EPR of Free Radicals in Solids. Trends in Methods and Applications”, Kluwer Scientific Publishing, 565–599 (2003).
15. [15] Yordanov, N.D., Lagunov, O., Dimov, K., “EPR spectra induced by gamma-irradiation of some dry medical herbs”, Radiation Physics and Chemistry 78: 277–280 (2009).
16. [16] Yordanov, N.D., “Quantitative EPR spectrometryState of the Art”, Appl. Magn. Res. 6: 241-257 (1994)
17. [17] Yordanov, N.Y., Aleksieva, K., Mansour, I., “Improvement of the EPR detection of irradiated dry plants using microwave saturation and thermal treatment”, Radiation Physics and Chemistry 73: 55–60 (2005).
18. [18] Swartz, H.M., Bolton, J.R., Borg, D.C. (Eds.), Biological Applications of Electron Spin Resonance, Wiley, New York (1972).
19. [19] Maloney, D.R., Tabner, B.J., Tabner, V.A., “An electron spin resonance study of gamma-irradiated fruits”, Radiat. Phys. Chem. 39: 309–314 (1992).
20. [20] Tabner, B.J., Tabner, V.A., “Electron spin resonance spectra of gamma-irradiated citrus fruits skin, skin components and stalks”, Int. J. Food Sci. Technol. 29: 143–152 (1994).
21. [21] Ikeya, M., Baffa, O.F., Mascarenhas, S., 1989. “Quality assessment of coffee beans with ESR and gamma-irradiation”, Appl. Radiat. Isotopes 40: 1219–1222 (1989).
22. [22] Braca, A., Sortino, C., Politi, M., Morelli, I., Mendez, J., “Antioxidant activity of flavonoids from Licania Licaniaeflora”, J. Ethnopharmacol, 79: 379-381 (2002).
23. [23] Ebrahimzadeh, M.A., Hosseinihmer, S.J., Hamidinia, A., Jafari, M., “Antioxidant and free radical scavenging activity of Feijoa Sallawiana fruits peel and leaves” Pharmacologyonline, 1: 7- 14 (2008).
24. [24] Beinert, H., Palmer, G., Adv. Enzymol. 27: 105 (1965).
25. [25] Morsy, M.A., Khaled, M.M., “Novel EPR characterization of the antioxidant activity of tea leaves”, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 58, 6: 1271-1277 (2002).
26. [26] Delincee, H., Soika, C., “Improvement of the ESR detection of irradiated food containing cellulose employing a simple extraction method”, Radiat. Phys. Chem. 63: 437–441 (2002).
27. [27] Desrosiers, M.F., McLaughlin, W.L., “Examination of gamma-irradiated fruits and vegetables by electron spin resonance specroscopy”, Radiat. Phys. Chem. 34: 895–898 (1989).