Salinity Tolerance of Durum Wheat Genotypes in Androgenesis

Abstract

The aim of this study was to investigate the response of certain old and modern durum wheat genotypes (Triticum durum Desf. Kunduru 1149, Berkmen 469, Mirzabey, Altın and Yılmaz) to salinity using anther culture technique. Anther response and callus production were investigated in genotypes applying without and after cold pretreatments on spikes, and significant differences were found in the results. Berkmen 469 produced higher amount of calli both without and after cold pretreatment (7.47% and 16.42%, respectively) than other genotypes. The percentage of responsive anthers and production of calli increased after cold pretreatment in all genotypes tested in this study.  Subsequently calli were transferred to the standard MS medium (control) and three different MS media containing various salt concentrations (NaCl doses of 0.5 mM, 1.0 mM and 1.5 mM). In this study, total plant regeneration was observed as 21.21% in Kunduru 1149 and 13.30% in Berkmen 469. Moreover, Kunduru 1149 succeeded in the plant regeneration on the MS medium modified by 0.5 mM and 1.0 mM NaCl concentrations.

 

Key words: Triticum durum Desf., salinity, androgenesis, plant regeneration

 

Keywords

• Triticum durum Desf., salinity, androgenesis, plant regeneration

References

1. Savaskan, C., Ellerbrook, C., Fish, L.J. and Snape, J.W., “Doubled haploid production in Turkish durum wheats crossing with maize”, Plant Breeding, 116, 299-301 (1997).
2. Razdan, M.K., Haploid Production, “An introduction to plant tissue culture”, Oxford and IBH. Pub., 105-124 (1992).
3. Ximings, L., Savaskan, C., Polok, K., Bielawska, A., Szarejko, I. and Maluszynski, M., “The effect of media and culture conditions on androgenetic response in barley, In: Reports Botanical Garden of the Polish Academy of Sciences”, Vol. 5/6. J.J. Rybczynski, I. Szarejko, J. Puchalski and M. Maluszynski (Eds.), pp. 487 – 495, Warsaw (1994).
4. Saidi, N., Cherkaoui, S., Chlyah, A. and Chlyah, H., “Embryo formation and regeneration in Triticum turgidum ssp. durum anther culture”, Plant Cell, Tissue and Organ Culture, 51, 27-33 (1997).
5. Savaskan, C., Szarejko, I. and Toker, M.C., “Callus production and plant regeneration from anther culture of some Turkish barley cultivars”, Turkish Journal of Botany, 23, 359-365 (1999).
6. Levitt, J., R”esponses of Plants to Environmental Stresses”, Vol. I Chilling, freezing, and high temperature stres, London, New York, Toronto: Academic Press, (1980).
7. Flowers, J. and Yeo, A.R., “Variability in the resistance of sodium chlorid salinity within rice (Oryza sativa L.) varieties”, New Phytol, 88, 363- 373 (1981).
8. Wang, X.Z., Hu, H., “The Effect of Potato II Medium for Triticale Anther Culture”, Plant Science Letter, 36, 237-239 (1984).

9. Bregitzer, P., “Plant regeneration and callus type in barley: Effects of genotype and culture medium”, Crop Sciences, 32, 1108-1112 (1992).
10. Doğramacı-Altuntepe, M., Peterson, T.S. and Jauhar, P.P., “Anther culture-derived regenerants of durum wheat and their cytological characterization”, The J. of Heredity, 92, 56-64 (2001).
11. Hassawi, D.S., Qrunfleh, I. and Dradkah, N., “Production of doubled haploids from some jordanian wheat cultivars via anther culture technique”, Journal of Food, Agriculture and Environment, 3, 161-164 (2005).
12. Lazar, M.D., Schaeffer, G.W. and Baezinger, P.S., “The physical environment in reletion to high frequency callus and plantet development in anther culture of wheat (Triticum aestivum L.) cv. “Chris””, Journal of Plant Physiology, 121, 103- 109 (1985).
13. Arzani, A. and Mirodjagh, S.S., “Response of durum wheat cultivars to immature embryo culture, callus induction and in vitro salt stres”, Plant Cell, Tissue and Organ Culture, 58, 67-72 (1999).