THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS

Savaş İzzetoğlu; Sabire Karaçalı

EN

THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS

Abstract

ABSTRACT

The effects of different concentrations of 20-hydroxyecdysone (20-E) on hemocytes of Galleria mellonella were investigated in vitro. Hemocyte culture was performed in Grace's insect medium with fetal calf serum. The hormone was added to the cultures at two different concentrations. During a period of three days, the cells (plasmatocytes, granulocytes and total cell number) were counted under an inverted microscope. Obtained numbers were evaluated with one way variance analysis (ANOVA). Hormone treatment led to a dose-dependent decrease in total cell and granulocyte number. The number of plasmatocytes, which are the predominant cells for phagocytose activity in immune response, was enhanced by hormone treatment.

Key Words: 20-hydroxyecdysone, hemocyte, in vitro, Galleria mellonella, insect

Keywords

20-hydroxyecdysone, hemocyte, in vitro, Galleria mellonella, insect

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Savaş İzzetoğlu This is me

Sabire Karaçalı This is me

Publication Date

August 6, 2010

Submission Date

August 6, 2010

Acceptance Date

-

Published in Issue

Year 2003 Volume: 16 Number: 2

IZ

https://izlik.org/JA27XJ92NX

Cite

RIS / Bibtex

APA

İzzetoğlu, S., & Karaçalı, S. (2010). THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS. Gazi University Journal of Science, 16(2), 233-238. https://izlik.org/JA27XJ92NX

AMA

1.İzzetoğlu S, Karaçalı S. THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS. Gazi University Journal of Science. 2010;16(2):233-238. https://izlik.org/JA27XJ92NX

Chicago

İzzetoğlu, Savaş, and Sabire Karaçalı. 2010. “THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS”. Gazi University Journal of Science 16 (2): 233-38. https://izlik.org/JA27XJ92NX.

EndNote

İzzetoğlu S, Karaçalı S (August 1, 2010) THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS. Gazi University Journal of Science 16 2 233–238.

IEEE

[1]S. İzzetoğlu and S. Karaçalı, “THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS”, Gazi University Journal of Science, vol. 16, no. 2, pp. 233–238, Aug. 2010, [Online]. Available: https://izlik.org/JA27XJ92NX

ISNAD

İzzetoğlu, Savaş - Karaçalı, Sabire. “THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS”. Gazi University Journal of Science 16/2 (August 1, 2010): 233-238. https://izlik.org/JA27XJ92NX.

JAMA

1.İzzetoğlu S, Karaçalı S. THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS. Gazi University Journal of Science. 2010;16:233–238.

MLA

İzzetoğlu, Savaş, and Sabire Karaçalı. “THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS”. Gazi University Journal of Science, vol. 16, no. 2, Aug. 2010, pp. 233-8, https://izlik.org/JA27XJ92NX.

Vancouver

1.Savaş İzzetoğlu, Sabire Karaçalı. THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS. Gazi University Journal of Science [Internet]. 2010 Aug. 1;16(2):233-8. Available from: https://izlik.org/JA27XJ92NX