The Application of 7-Chloro-4-Nitrobenzoxadiazole and 4-Fluoro-7-Nitro-2,1,3-Benzoxadiazole for The Analysis of Amines and Amino Acids Using High-Performance Liquid Chromatography

Abstract

This paper reviews analytical methods, instrumental developments and applications for derivatization of amines and amino acids with 7-Chloro-4-nitrobenzoxadiazole (NBD-Cl) and 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) using high performance liquid chromatography (HPLC). Discussion of the literature related to the application from 2001 to 2010 is presented.

Keywords

• NBD-Cl
• NBD-F
• HPLC
• 2D-HPLC
• Amines and Amino acids

References

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2. Shiseido) elution:isocratic, elutent: THF–TFA–water (25/0.05/75, v/v/v) For enantiomer separations, Chiralpak QN-AX and Chiralpak QD-AX (150 mm × 4.0 mm I.D., Chiral Technologies Europe, Illkirch, France): 10 mM citric acid in the mixed solution of MeOH–MeCN (50/50, v/v) Sample + 20 µl of mM borate buffer (pH 8.0) + 10 µl of 20 mM analytical column for the reversed-phase separation was a Mightysil RP-18 GP (100 mm × 1.0 mm I.D., Kanto Chemical, Tokyo, Japan), elution:isocratic, eluent: MeCN– THF–TFA–water (10:1:0.02:89, v/v) enantioselective column was a Sumichiral OA-2500S
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