Spectrofluorimetric Determination of Diiodohydroxyquinoline in Presence of Metronidazole in Pharmaceutical Formulation and Spiked Human Plasma

Year 2013, Volume: 26 Issue: 2, 141 - 149, 05.07.2013

Y Aboul-eneın , Nouruddin Alı Mohammed Gamal Mohammed Abdelkawy

https://izlik.org/JA76EW53DT

Abstract

Accurate and sensitive spectrofluorimetric method was developed for determination of diiodohydroxyquinoline in presence of metronidazole. In this method the native fluorescence of diiodohydroxyquinoline in water solvent at λ =495 nm when excitation was at 250 nm is used for its determination. Linear correlation was obtained in theconcentration range of 400 to 900 ng mL-1. The proposed method was successfully applied for determination ofdiiodohydroxyquinoline in bulk powder with mean accuracy of 100.21±1.13 or in spiked human plasma with mean accuracy of 100.53±1.42 without interference of metronidazole.

Keywords

spectrofluorimetry , Diiodohydroxyquinoline , Metronidazole

References

• 1. Merck Index: An Encyclopedia of Chemicals, Drugs and Biological 14th Ed., S. Budavari (Ed.), Merck & Co., Inc., Whitehouse Station, NJ(2006).
• 2. Martindale: The Extra Pharmacopoeia, the Complete Drug Reference 35th Ed., K. Profitt (Ed.), Royal Pharmaceutical Society, London, UK(2006).
• 3. United States Pharmacopeia "The National Formulary USP", United States Pharmacopeia convention Inc (2009).
• 4. Nagaraja, P, Sunitha, K R, Vasantha RA, Yathirajan H.S., Spectrophotometric determination of metronidazole and tinidazole in pharmaceutical preparations. Journal of Pharmaceutical and Biomedical Analysis 28(3-4), 527–535 (2002).
• 5. Erk N, Altun M L N., Spectrophotometric resolution of metronidazole and miconazole nitrate in ovules using ratio spectra derivative spectrophotometry and RPLC.Journal of Pharmaceutical and Biomedical Analysis, 25(1)115-122 (2001).
• 6. Khashaba PY, Refaat I H, Emara K M and Gaber H M. , Utility of Diazotized 4 - Amino-6-Chloro-1,3- Benzene Disulphonamide for the Spectrophotometric Determination of 8-Hydroxquinolines and Dibenzazepines.Analytical Letters, 32(15) 3029-3047 (1999).