PREPARATION OF FILM ELECTRODE USING DOPANT FOR CONSTRUCTION OF BIOSENSORS SENSITIVE TO HYDROGEN PEROXIDE

Year 2017, Volume: 30 Issue: 4, 104 - 112, 11.12.2017

Servet Çete Munise Özyurt Ertan Yildirim

Abstract

In this study, a new film
electrode for determination of hydrogen peroxide was developed. Polypyrrole-poly
(sodium-4-styrenesulphonate) film have been prepared on the platinum electrode
by the electropolymerization of pyrrole was carried out in the presence of
poly(sodium-4-styrenesulphonate). Optimum conditions of
polypyrrole-poly(sodium-4-styrenesulphonate) film was founded. Oxidation of the
hydrogen peroxide was examined at a different potential.
Therefore, four different potentials (0.4, 0.5, 0.6, and 0.7 V) were
determined. Also, concentration of pyrrole and poly(sodium-4-styrenesulphonate)
were examined. The effect of the number of cycles in their sensitivity to
hydrogen peroxide is investigated. Morphology of electrodes was qualified by means of SEM. Free enzyme work
was performed using the prepared film electrode and glucose oxidase. Linear
increases were observed in the amperometric response currents of the electrode
versus increasing glucose concentrations. It was shown that this film can be
used for hydrogen peroxide producing enzymes. 

Keywords

film electrode, hydrogen peroxide, polypyrrole, poly(sodium-4-styrenesulphonate)

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