In the study, new nanospheres support
containing ferrocene were synthesized and characterized, then the properties
of nanospheres as biocatalysis were researched. Novel support was prepared
from (Aminomethyl)polystyrene (AMP), tryptophan (Trp) and ferrocene aldehyde (Fc) and the
enzymatic properties of immobilized
glucose oxidase enzyme (GOx) was investigated. Modified
polystyrene was characterized IR spectra, Gel permeation chromatography and
Scanning electron microscopy. Ferrocene-tagged nanospheres-GOx
shows high reusability and storage capacity and fast incubation time
determination of glucose. After 12 months, immobilized GOx to the AMP-Trp
and AMP-Trp-Fc retained ca 20 % and
40 % for their original activity, respectively. High activities were found for
modified polymers AMP-Trp-Fc retains more than 35 % of the
initial activity after fifteenth successive cycles, which is a perfect performance.
Journal Section | Chemistry |
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Authors | |
Publication Date | December 11, 2017 |
Published in Issue | Year 2017 Volume: 30 Issue: 4 |