Preparation of Surface Imprinted Magnetic Nanoparticles for Selective Detection of Ciprofloxacin in Milk

Year 2017, Volume: 30 Issue: 4, 72 - 85, 11.12.2017

Adem Zengin

Abstract

Molecular
imprinted polymers were synthesized for selective detection of ciprofloxacin on
magnetic nanoparticles via surface initiated free radical polymerization.
Methacrylic acid, ethylene glycol dimethacrylate, azobisisobutyronitrile and
ciprofloxacin were used for the formation of molecular imprinted layer as a
functional monomer, cross linker, initiator and template molecule,
respectively. The surface characterization of the prepared nanoparticles were
carried out by using several surface characterization methods such as TEM, XPS
and VSM. The prepared imprinted nanoparticles had high binding capacity and
fast adsorption kinetics. Additionally, the prepared nanoparticles showed
superparamagnetic property with rapid magnetic separation and retained binding
selectivity after ten adsorption-desorption cycles. The imprinted magnetic
nanoparticles were subsequently applied for selective separation and
determination of CPX from milk with high recoveries (98.0% - 98.8%) and low
relative standard deviations (2.76% - 4.59%). All the results have shown that
the developed new method is a good alternative for the selective separation of
ciprofloxacin in complex medium.

Keywords

magnetic nanoparticles, molecular imprinting, magnetic separation, ciprofloxacin, milk

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