In this study, the adsorption conditions of Fe2+ ions on Saccharomyces cerevisae immobilized pumice stone were investigated by column method. In order toperform this aim, the effect of solution pH value, initial concentration of ions andsuitable solution and concentration for recovery of adsorbed ions wereinvestigated.At pH 5, the maximum adsorption was obtained. At optimal pH value,adsorption capacity of sorbent for metal was obtained as 1,81 mg.g-1, when initialconcentration was 60 mg.ml-1. Influence of HCl, NaCl, NH4Cl and EDTAsolutions on desorption were investigated and maximum recovery was achieved by0,2M EDTA solution. Desorption were 98% level provided by 0,2 M EDTAsolution.
Madrid, Y. and Camara , C., “Biological Substrates for Metal and Preconcentration and Speciation”, Trends in Anal. Chem., 16, 36-44 (1997).
Drake L.R., and Rayson G.D., “Plant-Derived Materials for Metal Ion-Selective Binding and Preconcentration”. Anal. Chem., 1, 22-27 (1996).
Aksu. Z. and Kutsal, T., “Bioseparation Process for Removing Lead(II) Ions from Waste Water by Using C. vulgaris”, J. Chem. Tech. Biotech., 52, 109-118 (1991). 2 4 6 Tekrar Kullanım Sayısı
No of adsorption-desorption cycle
Tsezos M., Baird H.I. and Shemilt, L.W., “The Kinetics of Radium Biosorption”, The Chem. Eng. J., 33, B35-B41 (1986).
Crist, R.H., Oberhaser, K. Shank, N., Nguyen, M., “Nature of Binding between Metallic Ions and Algal Cell Walls”, Environ. Sci. Tech., 15, 1212-1217 (1981).
Mahan, C.A. and Holcombe, J.A., “Immobilization of Algae Cells on Silica Gel and Their Characterization for Trace Metal Preconcentration”, Anal. Chem., 64, 1933-1940 (1992).
Bağ, H., Türker, A.R. and Lale, M., “Determination of Cu, Zn, Fe, Ni and Cd by Flame Atomic Absorption Spectrophotometry after Preconcentration by Escherichia coli Immobilized on Sepiolite”, Talanta, 51, 1035-1043 (2000)
Maquieria, A., Elmahadi, H.A.M. and Puchades, R., “Use of Saccharomyces cerevisiae in Flow Injection Atomic Absorption Spectrometry for Trace Metal Preconcentration”, Anal, Chem., 66, 1462-1467 (1994).
Gerin, P.A., Asther, M. and Rouxhet, P.G., “Peroxidase Production by the Filamentous Fungus Phanerochaete chrysosporium in Relation to Immobilization in “Filtering” Carriers”, Enzyme and Microbial Tech., 20, 294-300 (1997)
Garnham, G.W., Codd, G.A. and Gadd, G.M., “Accumulation of Cobalt, Zinc and Manganese by the Estuarine Green Microalga Chlorella salina Immobilized in Alginate Microbeads”, Environ. Sci. Tech., 26 (9), 1764-1770 (1992)
Elmahadi, H.A.M. Greenway, G.M., “Immobilization of Algae as a Reagent for Preconcentration in Trace Element Atomic Absorption Spectrometry”, J. Anal. At. Spect., 6, 643-651 (1991).
Maquieria, A., Elmahadi, H.A.M. and Puchades, R., “Technique and Support for Microorganism Immobilization Application to Trace Metals Enrichment by Flow Injection Atomic Absorption Spectrometry”, Analyst, 121, 1633- 1640 (1996).
Mahan, C.A. and Holcombe, J.A., “Preconcentration of Trace Metals Using Silica-Immobilized Algae Cells in a Chromatographic Separation Procedure”, Spectrochimica Acta, 47B, 1483-1490 (1992).
Madrid, Y., Cabrera, C., Corona, T. P., and Camara, C., “Speciation of Methyl Mercury and Hg(II) Using Saccharomyces cerevisiae, Determination by Continuous Flow Mercury Cold Vapor Generation Atomic Absorption Spectrometry”, Anal. Chem., 67, 750-754, (1995)
Suh, J.H., Yun, J.W. and Kim, D.S., “Comparison of Pb2+ Accumulation Characteristics Between Live and Dead Cells of Saccharomyces cerevisiae and Aureobasidium pullulans”, Biotechnology Letters, 20 (3), 247-251, (1998)
Bassari, A., Akyüz, T. and Kurtcebe, T., “The Removal of Th, Cs and Sr Ions from Solutions Using Granulated Pumice Stone”, J. of Inclusion Phenomena and Molecular Recognition in Chemistry, 26, 83-88, (1996)
Bruce M.M., “Ponza Pazarı”, M.T.A. Genel Müdürlüğü Fizibilite Etüdleri Dairesi, Ankara (1991).
Bağ, H., Lale, M. and Türker, A. R., “Determination of Iron and Nickel by Flame Atomic Absorption Spectrophotometry after Preconcentration on Saccharomyces cerevisiae Immobilized Sepiolite”, Talanta, 47, 689- 697 (1998).
Skoog, D.A. and West, D.M., “Fundamentals of Analytical Chemistry 3nded.” Holt Rinehart and Winston, USA, 790-791 (1976).
Elmahadi, H.A.M. Greanway, G.M., “Immobilization Algae as a Reagent for Preconcentration in Trace Element Atomic Absorption Spectrometry”, J. Anal. A. Spectrum., 6, 643-651 (1991).
Robles, L.C. and Aller, A.J., “Preconcentration of Beryllium on the Outer Membrane of Esherichia coli and Pseudomonas putida Prior to Determination by Electrothermal Atomic Absorption Spectrometry”, J. Anal. A. Spectrum., 9, 871-878 (1994)
Shengjun, M. and Holcombe, J.A., “Preconcentration of Copper on Algae and Determination by Slurry Graphite Furnace Atomic Absorption Spectrometry”, Anal. Chem., 62, 1994-1997 (1990)
Tsezos, M., Baird, M.H.I and Shemilt, L.W., “The Elution of Radium Adsorbed by Microbial Biomass”, The Chem. Eng. J., 34, B57-B62 (1987)
Nakajima, A., and Sakagucki, T., “Selective Accumulation of Heavy Metals by Microorganisms”, App. Microbiology and Biotech., 24, 59-65 (1986)
Tsezos, M., “Recovery of Uranium from Biological Adsorbents-Desorption Equilibrium”, Biotech. and Bioeng., 26, 973-981 (1984)
Lale, M., Temoçin, Z. and Bağ, H., “Sorption Behavior of Copper(II), Zinc(II) and Nickel(II) on Formaldehyde Cross-Linked Saccharomyces cerevisiae Immobilized on Pumice Stone ” Fresenius Environmental Bulletin, 10, 736-740 (2001)
Bu çalışmada kolon yöntemi kullanılarak Fe2+ iyonlarının Saccharomyces cerevisiae immobilize edilmiş ponza taşına adsorpsiyon şartları araştırıldı. Bu amaçla; adsorpsiyona çözelti pH sının, iyon başlangıç derişiminin ve adsorplanan iyonların geri kazanılması için uygun çözelti türü ve derişiminin etkisi araştırıldı. Maksimum adsorpsiyonun sağlandığı pH değerinin 5 olduğu belirlendi. Optimum pH değerinde, adsorbanın metal tutma kapasitesi 60 µg/mL başlangıç derişiminde 1,81mg/g dır. Desorpsiyona HCl, NaCl, NH4Cl ve EDTA çözeltilerinin etkisi araştırıldı. En uygun çözeltinin 0,2 M’lık EDTA çözeltisi olduğu gözlendi. 0,2 M EDTA çözeltisi ile %98 oranında desorpsiyon sağlandı
Madrid, Y. and Camara , C., “Biological Substrates for Metal and Preconcentration and Speciation”, Trends in Anal. Chem., 16, 36-44 (1997).
Drake L.R., and Rayson G.D., “Plant-Derived Materials for Metal Ion-Selective Binding and Preconcentration”. Anal. Chem., 1, 22-27 (1996).
Aksu. Z. and Kutsal, T., “Bioseparation Process for Removing Lead(II) Ions from Waste Water by Using C. vulgaris”, J. Chem. Tech. Biotech., 52, 109-118 (1991). 2 4 6 Tekrar Kullanım Sayısı
No of adsorption-desorption cycle
Tsezos M., Baird H.I. and Shemilt, L.W., “The Kinetics of Radium Biosorption”, The Chem. Eng. J., 33, B35-B41 (1986).
Crist, R.H., Oberhaser, K. Shank, N., Nguyen, M., “Nature of Binding between Metallic Ions and Algal Cell Walls”, Environ. Sci. Tech., 15, 1212-1217 (1981).
Mahan, C.A. and Holcombe, J.A., “Immobilization of Algae Cells on Silica Gel and Their Characterization for Trace Metal Preconcentration”, Anal. Chem., 64, 1933-1940 (1992).
Bağ, H., Türker, A.R. and Lale, M., “Determination of Cu, Zn, Fe, Ni and Cd by Flame Atomic Absorption Spectrophotometry after Preconcentration by Escherichia coli Immobilized on Sepiolite”, Talanta, 51, 1035-1043 (2000)
Maquieria, A., Elmahadi, H.A.M. and Puchades, R., “Use of Saccharomyces cerevisiae in Flow Injection Atomic Absorption Spectrometry for Trace Metal Preconcentration”, Anal, Chem., 66, 1462-1467 (1994).
Gerin, P.A., Asther, M. and Rouxhet, P.G., “Peroxidase Production by the Filamentous Fungus Phanerochaete chrysosporium in Relation to Immobilization in “Filtering” Carriers”, Enzyme and Microbial Tech., 20, 294-300 (1997)
Garnham, G.W., Codd, G.A. and Gadd, G.M., “Accumulation of Cobalt, Zinc and Manganese by the Estuarine Green Microalga Chlorella salina Immobilized in Alginate Microbeads”, Environ. Sci. Tech., 26 (9), 1764-1770 (1992)
Elmahadi, H.A.M. Greenway, G.M., “Immobilization of Algae as a Reagent for Preconcentration in Trace Element Atomic Absorption Spectrometry”, J. Anal. At. Spect., 6, 643-651 (1991).
Maquieria, A., Elmahadi, H.A.M. and Puchades, R., “Technique and Support for Microorganism Immobilization Application to Trace Metals Enrichment by Flow Injection Atomic Absorption Spectrometry”, Analyst, 121, 1633- 1640 (1996).
Mahan, C.A. and Holcombe, J.A., “Preconcentration of Trace Metals Using Silica-Immobilized Algae Cells in a Chromatographic Separation Procedure”, Spectrochimica Acta, 47B, 1483-1490 (1992).
Madrid, Y., Cabrera, C., Corona, T. P., and Camara, C., “Speciation of Methyl Mercury and Hg(II) Using Saccharomyces cerevisiae, Determination by Continuous Flow Mercury Cold Vapor Generation Atomic Absorption Spectrometry”, Anal. Chem., 67, 750-754, (1995)
Suh, J.H., Yun, J.W. and Kim, D.S., “Comparison of Pb2+ Accumulation Characteristics Between Live and Dead Cells of Saccharomyces cerevisiae and Aureobasidium pullulans”, Biotechnology Letters, 20 (3), 247-251, (1998)
Bassari, A., Akyüz, T. and Kurtcebe, T., “The Removal of Th, Cs and Sr Ions from Solutions Using Granulated Pumice Stone”, J. of Inclusion Phenomena and Molecular Recognition in Chemistry, 26, 83-88, (1996)
Bruce M.M., “Ponza Pazarı”, M.T.A. Genel Müdürlüğü Fizibilite Etüdleri Dairesi, Ankara (1991).
Bağ, H., Lale, M. and Türker, A. R., “Determination of Iron and Nickel by Flame Atomic Absorption Spectrophotometry after Preconcentration on Saccharomyces cerevisiae Immobilized Sepiolite”, Talanta, 47, 689- 697 (1998).
Skoog, D.A. and West, D.M., “Fundamentals of Analytical Chemistry 3nded.” Holt Rinehart and Winston, USA, 790-791 (1976).
Elmahadi, H.A.M. Greanway, G.M., “Immobilization Algae as a Reagent for Preconcentration in Trace Element Atomic Absorption Spectrometry”, J. Anal. A. Spectrum., 6, 643-651 (1991).
Robles, L.C. and Aller, A.J., “Preconcentration of Beryllium on the Outer Membrane of Esherichia coli and Pseudomonas putida Prior to Determination by Electrothermal Atomic Absorption Spectrometry”, J. Anal. A. Spectrum., 9, 871-878 (1994)
Shengjun, M. and Holcombe, J.A., “Preconcentration of Copper on Algae and Determination by Slurry Graphite Furnace Atomic Absorption Spectrometry”, Anal. Chem., 62, 1994-1997 (1990)
Tsezos, M., Baird, M.H.I and Shemilt, L.W., “The Elution of Radium Adsorbed by Microbial Biomass”, The Chem. Eng. J., 34, B57-B62 (1987)
Nakajima, A., and Sakagucki, T., “Selective Accumulation of Heavy Metals by Microorganisms”, App. Microbiology and Biotech., 24, 59-65 (1986)
Tsezos, M., “Recovery of Uranium from Biological Adsorbents-Desorption Equilibrium”, Biotech. and Bioeng., 26, 973-981 (1984)
Lale, M., Temoçin, Z. and Bağ, H., “Sorption Behavior of Copper(II), Zinc(II) and Nickel(II) on Formaldehyde Cross-Linked Saccharomyces cerevisiae Immobilized on Pumice Stone ” Fresenius Environmental Bulletin, 10, 736-740 (2001)
Lale, M., Şahin, N., & Temoçin, Z. (2010). ADSORPTION OF Fe2+ IONS WITH Saccharomyces cerevisiae IMMOBILIZED PUMICE STONE. Gazi University Journal of Science, 18(3), 365-373.
AMA
Lale M, Şahin N, Temoçin Z. ADSORPTION OF Fe2+ IONS WITH Saccharomyces cerevisiae IMMOBILIZED PUMICE STONE. Gazi University Journal of Science. August 2010;18(3):365-373.
Chicago
Lale, Mustafa, Nazan Şahin, and Zülfikar Temoçin. “ADSORPTION OF Fe2+ IONS WITH Saccharomyces Cerevisiae IMMOBILIZED PUMICE STONE”. Gazi University Journal of Science 18, no. 3 (August 2010): 365-73.
EndNote
Lale M, Şahin N, Temoçin Z (August 1, 2010) ADSORPTION OF Fe2+ IONS WITH Saccharomyces cerevisiae IMMOBILIZED PUMICE STONE. Gazi University Journal of Science 18 3 365–373.
IEEE
M. Lale, N. Şahin, and Z. Temoçin, “ADSORPTION OF Fe2+ IONS WITH Saccharomyces cerevisiae IMMOBILIZED PUMICE STONE”, Gazi University Journal of Science, vol. 18, no. 3, pp. 365–373, 2010.
ISNAD
Lale, Mustafa et al. “ADSORPTION OF Fe2+ IONS WITH Saccharomyces Cerevisiae IMMOBILIZED PUMICE STONE”. Gazi University Journal of Science 18/3 (August 2010), 365-373.
JAMA
Lale M, Şahin N, Temoçin Z. ADSORPTION OF Fe2+ IONS WITH Saccharomyces cerevisiae IMMOBILIZED PUMICE STONE. Gazi University Journal of Science. 2010;18:365–373.
MLA
Lale, Mustafa et al. “ADSORPTION OF Fe2+ IONS WITH Saccharomyces Cerevisiae IMMOBILIZED PUMICE STONE”. Gazi University Journal of Science, vol. 18, no. 3, 2010, pp. 365-73.
Vancouver
Lale M, Şahin N, Temoçin Z. ADSORPTION OF Fe2+ IONS WITH Saccharomyces cerevisiae IMMOBILIZED PUMICE STONE. Gazi University Journal of Science. 2010;18(3):365-73.