Comparison of Serum RSH, Immunoglobulin Levels and MPO Activity in Patients with Cystic Echinococcosis in Turkey

Abstract

The aim of this study was to elucidate the effect of larval form of Echinococcus granulosus on human serum antioxidant status and MPO activity and also serum immunoglobulin levels. For this research, sera of 361 patients were received who applied to Refik Saydam Hıfzıssıhha and Etlik SSK Hospital between 2003 and 2005 are suspected of the having Cystic Echinococcosis. It was found that 201 (55.7%) out of 361 patients were seropositive for the disease. Special reagent in the positive and control measured IgG, IgA and IgM levels. Total sulfhydryl groups (RSH) levels and the myeloperoxidase (MPO) activity was investigated by spectrophotometric methods. Although IgA levels increased in seropositive patients, IgG and IgM levels were not change in seropositive patient’s serum. Although MPO activity increased, RSH levels weren’t change seropositive patient’s serum. CE may alter immunoglobulin levels and antioxidant status of both female and male patients with E.granulosus infection.

Keywords

• Cystic Echinococcosis (CE)
• Indirect Hemaglutination (IHA)
• Immunoglobulin (Ig)
• RSH
• Myeloperoxidase (MPO).

References

1. Umur, S., “Prevalance and economic importence of Cystic Echinococcosis in slaughtered ruminants in Burdur, Turkey”, J. Vet. Met., 50, 247-252 (2003).
2. Altıntaş, N., “Past to present: Echinococcosis in Turkey”, Acta. Trop., 85,105–112 (2003).
3. Doğanay, A., “Paraziter hastalıklardan elde edilen ekonomik kayıplar”, Vet. Hek. Derg., 64, 52-59 (1993).
4. Özer, E., Özcan, C., Arslan, N., Kalendar, H., Angın, M., “Elazığ Et ve Balık Kurumunda atılan koyun karaciğerlerinde bakteriyel ve paraziter etkenlerle bunların oluşturduğu ekonomik kayıplar”, Tr. J. Vet. Anim. Sci., 20,191-201 (1996).
5. Torgerson, P. R., Dowling, P. M., “Estimating the economic effects of Cystic Echinococcosis. Part 2. an endemic region in the United Kingdom, a wealthy industrial economy”, Ann. Trop. Med. Parasitol., 95, 177-185 (2001).
6. Çivi, S., Guler, S., “Kist Hidatik hastalığı nedeniyle oluşan ekonomik kayıplar”, T. Parasitol. Derg., 19, 230–236 (1995).
7. Calegaro,L., Zambon, V., Montella, T., Loli Piccolomini “Echinococcosis/Hydatidosis: assessment of hospital costs”, Inf. Circ. WHO Moditerr. Zooom. Cont., 40, 8-11 (1996). G., the for
8. Torgerson, P. R., Dowling, P. M., Abo-Shedada, M. N., “Estimating the economy effects of Cystic Echinococcosis. Part 3. Jordan, a developing country with lower-middle income”, Ann.Trop. Med. Parasitol., 95, 595-603 (2001).

9. Sbihi, Y., Rmiqui, A., Rodriguez-Cabezas, M. N., Orduna, A., Rodriguez- Torres, A., Osuna, A., “Comparative sensitivity of six serological tests and diagnostic value of ELISA using purified antigen in hydatidosis”, J. Clin. Lab. Anal., 15, 14–18 (2001).
10. Kagan, I. G., “A review of serological tests for the diagnosis of hydatid disease”, Bull. World Health Organ, 39, 25–37 (1968).
11. Craig, P. S., Gasser, R. B., Pwrad, L., Cabrera, P., Parietti, S., Borgues, C., Acuttis, A., Agula, J., Snowden, K., Paolillo, E., “Diagnosis of canine Echinococcosis: Comparisons of coproantigen and serum antibody tests with are coline purgation in Uruguay”, Veterinary Parasitol., 56, 293-301 (1995).
12. Hernández, A., Cardozo, G., Dematteis, S., Baz ,A., Trias, N., Nuñez, H., Barragué, A., López, L., Fuentes, J., López, O., Ferreira, C., “Cystic Echinococcosis: analysis of the serological profile related to the risk factors in individuals without ultrasound liver changes living in an endemic area of Tacuarembó, Uruguay”, Parasitology, 130, 455–60 (2005).
13. Barış, İ., Şahin, A., Bilir, N., Kalyoncu, A. F., Emri, A. S., Selçuk, Z. T., “Hidatik kist hastalığı ve Türkiye 'deki konumu", Türkiye Akciğer Hastalıkları Vakfı Yayını, Ankara, 25-36 (1989).
14. Eberhardt, M. K., “Reactive Oxygen Metabolites”, CRC Pres,, New York, 24 (2001).
15. Stadtman, E.R. “In Free Radicals, Oxidative Stress, and Antioxidants: Pathology and Physiological Significance”, T. Özben, Ed., Plenum Press: New York, 131-143 (1998).
16. Evans, W. J., “Vitamin E, vitamin C, and exercise”, Am. J. Clin. Nutr,, 72, 6478-6528 (2000).
17. Casa. C. L., Villegas, I., Lastra, A., Motilva, V., Calero, M. J. M., “Evidence for protective and antioxidant properties of rutin, a natural flavone, aganist ethanol induced gastric lesions”, Journal of Ethnopharmacology, 71, 45–53 (2000).
18. Abidova, S. S., Ovchinnikov, I. V., Shamirzaev, B. I., Ishankulova, G. F., “The effect of alpha-tocopherol on blood lipid peroxidation in patients with hepatic echinococcosis”, Eksp. Klin. Farmakol. Russian, 61, 41-42 (1998).
19. Gaafar, M. R., Eissa, M. M., Ashram, Y. A., Diab, I. H., “Experimental electrophysiological and biochemical studies”, J. Egypt Soc. Parasitol., 36, 455-66 (2006). M. parasitological,
20. Klebanoff, J. S., “Myeloperoxidase: friend and foe”, Journal of Leukocyte Biology, 77, 598-625 (2005).
21. Van Gool, T., Vetter, H., Vervoort, T., Doenhoff, M. J., Wetsteyn, J., Overbosch, D., “Serodignosis of imported schistosomiasis by a combination of a commercial indirect hemagglutination test with Schistosoma mansoni adult worm antigens and enzyme-linked immunosorbent assay with S mansoni egg antigens”, L. Clin. Microbiol., 40, 3432–3437 (2002).
22. Elmann, G. L., “Tissue sulyphydryl groups”, Arch. Biochem. Biophys., 82, 70-77 (1959).
23. Glowick, S. P., Kaplan, S. D., “Methods in Enzymology”, Academic Press., New York, 769 (1955).
24. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J., “Protein measurement with the Folin phenol reagent”, J. Biol. Chem., 193, 265-75 (1951).
25. Wattal, C., Malla, N., Khan, I. A., Agarwal, S. C., “Comparative immunosorbent assay for the diagnosis of pulmonary echinococcosis”, J. Clin. Microbiol., 24, 41–46 (1986). of enzyme-linked
26. Safioleas, M., Manti, C., Misiakos, E., “Surgical treatment for splenic hydatidosis”, World J. Surg., 21:374-377 (1997).
27. Safioleas, M., Misiakos, E.P., Kakisis, J., “Surgical treatment of human echinococossis”, In.t Surg., 85:358-365 (2000).
28. Safioleas, M., Stamoulis, I., Theocharis, S., Moulakakis, K., Makris, S., Kostakis, A., “Primary hydatid disease of the gallbladder: a rare clinical entity”, J. Hepatobiliary Pancreat. Surg., 11:352– 356 (2004).
29. Safioleas, M.C., Moulakakis, K.G., Manti, C., Kostakis, A., “Clinical considerations of primary hydatid disease of the pancreas”, Pancreatology, 5:457-461 (2005).
30. Doty, J.E., Tompkins R.K., “Management of cystic disease of the liver”, Surg. Clin. North Am.,69: 285– 295 (1989).
31. Langer, J.C., Rose, D.B., Keyston, J.S., Taylor, B.R., Langer. B., “Diagnosis and management of hydatid disease of the liver: a 15-year North American experience”, Ann. Surg., 199:412–417 (1984).
32. Tsaroucha, A. K.,
33. Polychronidis, A. C.,
34. Lyrantzopoulos, N., “Hydatid disease of the abdomen
35. and other locations”, World J Surg., 29:1161–1165 (2005).
36. Richard, M. D., “Serological diagnosis and post- operative surveillance of human hydatid disease. Latex agglutination and immunoelectrophoresis using crude cyst fluid antigen”, Pathology, 16:207-210 (1984).
37. Force, L., Torres, J. M., Carrillo, A., Buscà, J., “Evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up”, Clin. Infect. Dis., 15:473-480 (1992).
38. Bulut, V., İlhan, F., Yücel, Y. Y., Onal, S., İlhan Y., Gödekmerdan, A., “Immunological fllow-up of hydatid cyst cases”, Mem. Ins.t Oswaldo Cruz., 96:669-671 (2001).
39. Kozłowska-Łój, J., Rzymowska, J., “Infection with Echinococcus expression of superoxide dismutase gene at mRNA level in hepatocytes”, Wiad. Parazytol., 52, 287–9 (2006). (Batsch, 1786) and
40. Tischendorf, F. W., Brattig, N. W., Büttner, D.W., Pieper, A., Lintzel, M., “Serum levels of eosinophil cationic protein, eosinophil-derived neurotoxin and myeloperoxidase in infections with filariae and schistosomes”, Acta. Trop., 62:171–82 (1996).