The Effect of Rheum ribes Extract Origin of Elazig Province on Ventilator-Associated Pneumonia and Antioxidant Capacity

Abstract

Ventilatör ilişkili pnömoni (VİP), yoğun bakım ünitesindeki (YBÜ) en önemli enfeksiyonlardan biridir ve hastalarda mortalite ve morbidite artışına katkıda bulunur. Bu çalışmada Rheum ribes (Elazığ/Türkiye) ekstraktının VİP hastalarından elde edilen bakteri izolatları üzerinde antimikrobiyal etkilerini (agar kuyu difüzyonu, MİK testi) ve antioksidan kapasitesini (DPPH, FRAP ve metal şelatlama) değerlendirmeyi amaçladık. Analiz sonucunda R.ribes'in en yüksek antimikrobiyal etkisi 19.32 mm zon çapı ile Klebsiella pneumonia-2 (K.pneumonia) ve 18.45 mm zon çapı ile K.pneumonia-21 izolatında gözlendi. Bunların dışında Staph. aureus., K. pneumonia-20, K. pneumonia-16 ve Pseudomonas aeruginosa (P. aeruginosa) sırasıyla 18.32, 15.14, 14.56 ve 13.54 mm zon çapları ile tespit edildi. R. ribes ekstraktı K.pneumonia izolatları 16 ve 20'de 0,5 ppm'de %98,3 ve %94,88 inhibitör etki gösterirken, en yüksek izolatlar 21 ve 2'de %100 inhibitör etki göstermiştir. S. aureus %50,36 gibi yüksek bir inhibisyon etkisi göstermiştir. 0,25 ppm'de ve P. aeruginosa 0,5 ppm'de %82,82 oranında izole edilmiştir. Ayrıca DPPH, FRAP ve metal şelasyon analizleri güçlü bir antioksidan etki göstermiştir. Ribes ekstraktının antioksidan etkileri için demir iyonlarının (Fe2+) DPPH inhibisyon etkisi, FRAP analizi ve şelatlama aktivitesi değerleri sırasıyla %17.22, %1.18 ve %2.14 olarak belirlendi. Sonuç olarak, R. ribes ekstraktının patojenik bakteriler üzerindeki güçlü etkisi ve antioksidan kapasitesi belirlenmiş olup, in vivo ve klinik çalışmalarla faydalı özellikleri derinleştirilebilir.

Keywords

• Rheum ribes
• ventilator associated pneumonia
• antimicrobial activity
• antioxidant capacity

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