İsatinin HepG2 ve AML12 hücre hatları üzerindeki moleküler etkilerinin araştırılması

Year 2024, , 394 - 401, 15.06.2024

Deniz Şumnulu

https://doi.org/10.17714/gumusfenbil.1389743

Abstract

İsatin, indol türevi olan organik bir bileşiktir. Couroupita guianensis bitkisinin doğal bir bileşenidir. İnsan vücudu hormonu olan adrenalinin de metabolik türevidir. Yapılan çalışmalarda isatin türevlerinin anti-tümöral etkileri gösterilmiştir. Bu çalışmada isatinin hepatoselüler kanser hücre hattı olan HepG2 üzerindeki sitotoksik etkileri ve sağlıklı karaciğer hücre hattı olan AML12 üzerindeki sitotoksik, koruyucu-proliferatif etkileri MTT, floresan boyama, yara iyileşmesi ve gerçek zamanlı polimeraz zincir reaksiyonu analizleri ile araştırıldı. 48 saatlik isatin uygulaması için IC50 değerleri HepG2 için 186.23 µM, AML12 için 7.05 mM olarak hesaplandı. Her iki hücre hattı için HepG2 uygulamasının IC50 dozuna bağlı olarak, yara iyileşmesi ve floresan boyama analizleri, HepG2 hücrelerinde proliferasyonun baskılandığını ve apoptozun tetiklendiğini, ancak AML12 hücrelerinde proliferasyonun teşvik edildiğini göstermiştir. Ek olarak, oksidatif stres genleri CuZn/Mn-SOD ve mitokondriyal apoptotik yol genleri Bax, kesilmiş-Cas3, APAF1 ve p53'ün ekspresyon seviyeleri HepG2'de artmış ancak AML12 hücre hatlarında azalmıştır. Bu çalışmalar sonucunda isatinin doğal formunun karaciğer kanseri hücre dizileri için iyi bir anti-kanser ajanı ve sağlıklı karaciğer hücreleri için koruyucu bir takviye olabileceği sonucuna varılmıştır.

Keywords

AML12, Anti-kanser, Sitotoksik, HepG2, İsatin

Investigation of the molecular effects of isatin on HepG2 and AML12 cell lines

Abstract

Isatin is an indole-derived organic compound. It is a natural component of the Couroupita guianensis plant. It is also the metabolic derivative of the human body hormone adrenaline. Studies have shown the anti-tumoral effects of isatin derivatives. In this study, the cytotoxic effects of isatin on HepG2, a hepatocellular cancer cell line was investigated. Additionally, its cytotoxic and protective-proliferative effects on AML12, a healthy liver cell line was investigated. This evaluation was conducted using MTT, fluorescent staining, wound healing, and real-time polymerase chain reaction analyses. The IC50 values for 48 hours of isatin application were calculated as 186.23 µM for HepG2 and 7.05 mM for AML12. The analysis of wound healing and fluorescent staining at varying doses of HepG2 application revealed suppression of proliferation and triggered apoptosis in HepG2 cells. In contrast, AML12 cells exhibited promoted proliferation under similar conditions. Moreover, the observed upregulation of oxidative stress genes CuZn/Mn-SOD and mitochondrial apoptotic pathway genes Bax, cleaveled-Cas3, APAF1, and p53 in HepG2 cells contrasted with their decreased expression in AML12 cell lines. These results suggest the potential of natural isatin as a promising anti-cancer agent for liver cancer cell lines and as a protective supplement for healthy liver cells.

Keywords

AML12, Anti-cancer, Cytotoxic, HepG2, Isatin

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