İsrail Sazanı (Carassius gibelio Bloch, 1782) Balıklarına Uygulanan Cypermethrin’in Oksidatif Stres Parametreleri Üzerine Etkileri

Year 2016, , 41 - 46, 31.01.2016

Hamit Uslu , Gözde Atila , Yusuf Ersan , Zaide Özden

https://doi.org/10.17714/gufbed.2016.06.005

Cited By: 1

Abstract

Bu çalışmada 96 saat boyunca düşük (1 µgL^-1), orta (1.5 µgL^-1) ve yüksek (2 µgL^-1) dozlarda Cypermethrin maruziyetinde Carassius gibelio’nun karaciğer, böbrek ve kas gibi farklı dokuları ile serum örneklerindeki oksidatif stres belirteçlerinden olan Malondialdehit (MDA) ile Glutatyon (GSH) ve Süperoksit Dismutaz (SOD) gibi antioksidanların seviyelerinin değişikliklerini belirlemek amaçlanmıştır. Bulgular MDA’nın karaciğer ve kas dokuları (tüm gruplarda; p<0.001 düzeyinde) ile serum örneklerinde (sırasıyla; p<0.01, p<0.001, p<0.001) önemli şekilde artırdığını göstermiştir. SOD seviyelerinin karaciğer (sırasıyla; p<0.05, p<0.01, p<0.001) ve kas dokuları (orta ve yüksek dozlarda; p<0.001 düzeyinde) ile serum örneklerinde (yüksek dozda; p<0.01 düzeyinde) önemli şekilde azaldığı belirlenmiştir. Ayrıca GSH seviyelerinin de kas (sırasıyla; p<0.001, p<0.001, p<0.05) ve serum örneklerinde (yüksek dozda; p<0.01 düzeyinde) önemli şekilde azaldığı tespit edilmiştir. Böbrek dokusunun oksidan ve antioksidan seviyelerinde önemli bir değişiklik gözlemlemedik (p>0.05). Cypermethrin gibi pestisitlerin kontrolsüz, bilinçsiz ve gereksiz kullanımı çevre ve halk sağlığında ciddi tehlikelere yol açabilmektedir. Bu çalışmanın sonuçları kısa süreli Cypermethrin maruziyetinde bile oksidan ve antioksidanlar arasındaki dengede oldukça önemli bozulmalar olduğunu göstermektedir.

Keywords

Antioksidan, Oksidan, Oksidatif stres, Carassius gibelio, Cypermethrin

The Effect Of Cypermethrin On Oxidative Stress Parameters In Israeli carp (Carassius gibelio Bloch, 1782) Fish

Abstract

The present study was aimed to determine changes in the levels of oxidative stress markers such as Malondialdehyde (MDA) and the antioxidant levels such as Glutathione (GSH) and Superoxide Dismutase (SOD) in different organs such as liver, kidney, muscle, and serum samples of Carassius gibelio exposure to low (1 µgL^-1), middle (1.5 µgL^-1) and high (2 µgL^-1) doses Cypermethrin for 96 h. The results showed significant increase in MDA of the liver and muscle tissues (p<0.001 level in all groups) with serum samples (p<0.01, p<0.001, p<0.001 respectively). SOD levels was determined that the significant decrease in liver (p<0.05, p<0.01, p<0.001 respectively), muscle tissues (p<0.001 level in medium and high doses) and serum samples (p<0.01 level in high dose). Moreover GSH levels was detected that significant decrease in the muscle (p<0.001, p<0.001, p<0.05 respectively) and serum samples (p<0.01 level in high doses). We did not observe a significant difference of the oxidant and antioxidants levels in the kidney tissue (p>0.05). The uncontrolled, insensible and unnecessary usage of pesticides such as Cypermethrin can be use serious hazard to the environment and public health. The result of the study showed that the very important corruption balance between oxidants and antioxidants depend on Cypermethrin exposured even in short period.

Keywords

Antioxidant, Oxidant, Oxidative stress, Carassius gibelio, Cypermethrin

References

• Boxaspen, K., ve Holm, J.C., 2001. The development of pyrethrum-based treatments against the ectoparasitic salmon lice Lepeophtheirus salmonis in sea cage rearing of Atlantic salmon Salmo salar L, Aquaculture Research, 32 (9): 701–707.
• Bradbury, S.P., ve Coats, J.R., 1989a. Comparative toxicology of the pyrethroid insecticides, Bulletin of Environmental Contamination and Toxicology, 108: 134–177.
• Bradbury, S.P., ve Coats, J.R., 1989b. Toxicokinetics an toxicodynamics of pyrethroid insecticides in fish, Environmental Toxicology and Chemistry, 8 (5): 373–380.
• Burr, S.A., ve Ray, D.E., 2004. Structure-activity and interaction effects of 14 different pyrethroids on voltage-gated chloride ion channels, Toxicologycal Sciences, 77 (2): 341-6.
• Chakraborty, K., Joseph, D., Stephy, P.S., Chakkalakal, S. J., Joy, M. ve Raola, V. K., 2014. Inter annual variability and seasonal dynamics of amino acid, vitamin and mineral signatures of ribbon fish, Trichiurus lepturus (Linnaeus, 1758), International Food Research Journal, 21 (5): 2007–2016.
• Cossu, C., Doyotte, A., Babut, M., Exinger, A., ve Vasseur, P., 2000. Antioxidants biomarkers in freshwater bivalves, Unio tumidus, in response to different contamination profiles of aquatic sediments, Ecotoxicology and Environmental Safety, 45: 106–121.
• Çelikel, Y., 2011. Alpha-Cypermethrin'in Daphnia manga (Straus 1820) (Cladocera, Crustacea) üzerine akut toksik etkisinin araştırılması. Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü. Ankara, 57s.
• Franco, J.L., Trivella, D.B.B., Trevisan, R., Dinslaken, D.F., Marques, M.R.F., Bainy, A.C.D., ve Dafre, A.L., 2006. Antioxidant status and stress proteins in the gills of the brown mussel Perna perna exposed to zinc, Chemico-Biological Interactions, 160 (3): 232–240.
• Giray, B., Gürbay, A., ve Hincal, F., 2001. Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol, Toxicology Letters, 118: 139–146.
• Hart, J.L., Thacker, J.R.M., Braidwood, J.C., Fraser, N.R., ve Mattews, J.E., 1997. Novel cypermethrin formulation for the control of sea lice on salmon (Salmo salar), Veterinary Records, 140 (7): 179–181.
• Hayes, A.W.,1994, Principles and Methods of Toxicology. 3rd Edition, New York, Raven Press, 435p.
• Hill, J.R., 1989. Aquatic organisms and pyrethroids, Pesticide Science, 27 (4): 429-465.
• Huggett, R.J., Kimerle, RA., Mehrle, P.M., ve Bergman, H.L., 1992. Biochemical, physiological and histological markers of anthropogenic stress. Boca Raton, Lewis.
• Jin, Y., Zheng, S., Pu, Y., Shu, L., Sun, L., Liu, W., Fu, Z., 2011. Cypermethrin has the potential to induce hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish (Danio rerio), Chemosphere, 82 (3): 398-404.
• Kehrer, J.P., 1993. Free radicals as mediators of tissue injury and disease, Critical Reviews in Toxicology, 23 (1): 21-48.
• Köprücü, K., Yonar, S.M., ve Şeker, E., 2010. Effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unio elongatulus eucirrus, Fisheries Science, 76 (3): 1007-1013.
• Livingstone, D.R., 2001. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms, Marine Pollution Bulletin, 42 (8): 656–666.
• Placer, Z.A., Cushman, L.L., ve Johnson, B.C., 1966. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems, Analytical Biochemistry, 16: 359–64.
• Richards, R.H., 1983. Diseases of farmed fish: Salmonids, Veterinary Records, 112 (6): 124–126.
• Roth, M., Richards, R.H., ve Sommerville, C., 1993. Current practices in the chemotherapeutic control of sea lice infestations in aquaculture: a review, Journal of Fish Diseases, 16 (1): 1–26.
• Sedlak, J., ve Lindsay, R.H., 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent, Analytical Biochemistry, 25: 192–205.
• Shashikumar, S., ve Rajini, P.S., 2010. Cypermethrin-induced alterations in vital physiological parameters and oxidative balance in Caenorhabditis elegans, Pesticide Biochemistry and Physiology, 97: 235–242.
• Treasurer, J.W., ve Wadsworth, S.L., 2004. Interspecific comparison of experimental and natural routes of Lepeophtheirus salmonis and Caligus elongatus challenge and consequences for distribution of chalimus on salmonids and therapeutant screening, Aquaculture Research, 35 (8): 773–783.
• Valavanidis, A., Vlahogianni, T., Dassenakis, M., ve Scoullos, M., 2006. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants, Ecotoxicology and Environmental Safety, 64: 178–189.
• Zhao, M., Zhang, Y., Wang, C, Fu, Z., Liu, W., ve Gan, J., 2009. Induction of macrophage apoptosis by an organochlorine insecticide acetofenate, Chemical Research in Toxicology, 22 (3): 504–510.