Glikoz İzomeraz Üreticisi Dört Geobacillus Suşunun İzolasyonu ve Moleküler Metotlar Kullanarak Tanımlanması

Year 2018, , 299 - 308, 31.07.2018

Kadriye İnan Bektaş

https://doi.org/10.17714/gumusfenbil.319426

Abstract

Türkiye'nin
batısındaki iki kaplıca, termofilik mikroorganizmaların mevcudiyetine göre
araştırıldı ve iki termal kaplıcadan,23 termofilik bakteri izole edildi.
İzolatlardan, 4 tanesinin glikoz izomeraz üretimi açısından pozitif olduğu
belirlendi. BT5, CT6SARI ve CT1.2 izolatlarının glikoz
izomerazları, pH 6.5'te optimum büyüme gösterdi; buna karşılık izolat BT1
enzimi, pH 7.5'te optimal aktivite gösterdi. Bu enzimler arasında BT1
izolatının optimal büyüme sıcaklığı 75 °C olarak belirlenirken, diğer üç
izolatın enzimleri ise 80 °C'de optimal büyüme gösterdi. 16S rRNA gen dizi
analizleri ile 4 izolatın, Geobacillus
cinsine ait türler oldukları belirlendi. recN
dizisi benzerlik analizine dayanarak, izolat CT6SARI’nın,
G. termodenitficans’a; izolat BT1’in
de, G. stearothermophilus’a ait
suşlar oldukları belirlendi.BT5 ve CT1.2 izolatlarının kesin tür tayinleri
yapılamadı.

Keywords

Geobacillus, Glikoz izomeraz, recN, 16S rRNA

Isolation of Four Glucose Isomerase Producing Strains of Geobacillus and Their Identification Using Molecular Methods

Abstract

Two hot springs located in west of
Turkey were investigated with respect to presence of thermophilic microorganisms. 23 thermophilic bacteria were
isolated from two hot springs. Among these, 4 strains were found positive for
glucose isomerase production. The
glucose
isomerases of strains BT5,
CT6SARI and CT1.2 showed
optimal growth at pH 6.5, whereas the enzyme of strain BT1 represented optimal
activity at pH 7.5. The strain BT1 had the lowest temperature optima (75°C)
among these strains whereas the other three strains showed optimal growth at 80
°C. On the basis of the 16S rRNA gene sequence analysis, the 4 isolates are
members of the genus Geobacillus.
Based on recN sequence similarity analysis, the isolate CT6SARI is a strain of G. thermodenitficans. The isolate BT1 is a strain of G. stearothermophilus. The species
identity for the isolates BT5 and CT1.2are uncertain.

Keywords

Geobacillus, Glucose-isomerase, recN, 16S rRNA

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