Caltha palustris L. Bitkisinin Çiçek ve Yaprak Kısmının Uçucu Yağlarının Kimyasal Bileşimi ve Antimikrobiyal Aktiviteleri

Year 2019, , 189 - 195, 15.04.2019

Tayyibe Beyza Yücel , Seda Fandaklı Salih Terzioğlu , Nurettin Yaylı

https://doi.org/10.17714/gumusfenbil.399711

Abstract

Caltha palustris L. (Ranunculaceae) bitkisinin çiçek ve yaprak kısımlarının uçucu
yağları Clevenger aparatlı  subuharı
destilasyonu yöntemiyle elde edilmiştir. Elde edilen uçucu yağların kimyasal
bileşenleri, GC-FID ve GC-MS teknikleriyle aydınlatılmıştır. C. palustris
bitkisinin çiçek ve yaprak kısımlarının uçucu yağlarında sırasıyla; toplam 52
ve 36 bileşik bulunmuş olup; sırasıyla %95.34 ve %82.4’lük kısımları
aydınlatılmıştır. Çiçek kısmının uçucu yağının başlıca bileşenleri; oktadekanol
(%25.40), fitol (%18.56), octanol (%9.01), neofitadien (%3.65), alkol (%36.30)
ve terpenoit (%22.62) bileşiklerden oluştuğu; yaprak kısmının uçucu yağ
analizinde ise ise heptadekan (%10.05), fitol (%8.94), oktadekanol (%6.3),
heneikosan (%3.03), (E)-β-farnesan (%1.34) gibi ağırlıklı olarak hidrokarbon
(%30.89) ve terpenoit (%14.59) bileşiklerden oluştuğu tespit edilmiştir C. palustris
bitkisinin çiçek ve yaprak kısımlarından elde edilen uçucu yağların
antimikrobiyal aktiviteleri, 8 adet Gram pozitif ve Gram negatif bakteri ve
mantarlara karşı araştırılmıştır. Uçucu yağların, Candida albicans ve Saccharomyces cerevisiae’ya (8-14mm) karşı antimikotik aktivite gösterdiği tespit edilmiştir.

Keywords

Antimikrobiyal aktivite, Caltha palustris, GC-FID/MS, Uçucu yağ

Chemical Composition and Antimicrobial Activity of Essential Oil From the Flower and Leaf of Caltha palustris L.

Abstract

The essential oils from flower and leaf of Caltha palustris
L. were obtained by hydrodistillation with Clevenger-type apparatus and
analyzed by GC-FID and GC-MS. Fifty-two compounds in the oil resulted from
flower of C. palustris, representing 95.34% and thirty-six compounds in the oil
obtained from leaf of C. palustris, representing 82.4%, were identified. The
main components of the volatile oil of the flower part are octadecanol
(25.40%), phytol (18.56%), octanol (9.01%) and neophytadien (3.65%) which are
alcohols (36.30%) and terpenoids (22.62%) type compounds. It was found that the
volatile oil of the leaf part consisted predominantly of hydrocarbons (30.89%)  and terpenoid (14.59%)  compounds such as heptadecane (10.05%), phytol
(8.94%), octadecanol (6.3%), heneicosane (3.03%) and  (E)-β- farnesane (1.34%) were major
constituents. The antimicrobial activities of the essential oils obtained from
flower and leaf parts of C. palustris plant were investigated against 8 Gram
positive and Gram-negative bacteria and fungi. It has been determined that
essential oils have antimycotic activity against Candida albicans and Saccharomyces cerevisiae (8-14 mm),
respectively.

Keywords

Antimicrobial activity, Caltha palustris, GC-FID/MS, Essential oil

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