Investigation of Antimicrobial, Antioxidant, and Antimutagenic Activities of Wild Guelder RoseFruit Extract Grown in Ardahan and Its Surrounding Area

Year 2018, Volume: 8 Issue: 1, 18 - 25, 31.01.2018

Mehmet Arslan Nurcan Erbil Zehra tuğba Murathan

https://doi.org/10.17714/gumusfenbil.294719

Cited By: 3

Abstract

In this study, antioxidant activity, antibacterial effect against some
test bacteria, and antimutagenic effect with AMES test of Guelder rose fruit
naturally growing in Ardahan and its surrounding area were examined. Different
Gr (+), and Gr (-) bacteria and yeasts were used as test microorganisms during
the antibacterial activity tests. AMES / Salmonella/Microsome test was
preferred for antimutagenic effect study and Salmonella typhimurium TA 98 and
TA 100 strains were used. According to the obtained data, 0.80 g fruitweight on
average, 11.3% of Soluble Dry Matter content, 3.20 of pH, 0.80 mg/g of total
anthocyanin, and 52.5% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical
scavenging activity, 72.8 µmol TE/g FW 2,2′-Azino-bis
(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) 
value, 122.6 µmol Fe II/g FW Ferric Reducing Antioxidant Power (FRAP)
value were determined. It was determined that Guelder rose fruit extract has
antibacterial activity at different rates on test bacteria, but not antifungal
activity. The most sensitive bacterium was Pseudomonas aeroginosa ATCC 9027
among the test microorganisms. In antimutagenic activity, only 40 μl dose of
Guelder rose in TA98 strains caused a significant reduction compared to the
positive control. In TA100 strains, no statistically significant decrease compared
to positive control at any dose occurred.

Keywords

Antimicrobial, Antimutagenic, Antioxidant, Guelder rose, Viburnum opulus

Ardahan ve Çevresinde Yabani Olarak Yetişen Gilaburu Meyve Ekstraktının Antimikrobiyal, Antioksidan ve Antimutajenik Aktivitelerinin Araştırılması

Abstract

Bu çalışmada Ardahan ve çevresinde doğal olarak
yetişmekte olan Gilaburu meyvelerinin antioksidan özellikleri, farklı
mikroorganizmalar üzerindeki antimikrobiyal etkisi ve Ames testi ile
antimutajenik etkisi araştırıldı. Antimikrobiyal aktivite testleri esnasında
farklı Gr (+) ve Gr (-) bakteriler ile mayalar kullanıldı. Antimutajenik etki
çalışmalarında ise AMES/Salmonella Mikrozom testi ile Salmonella typhimurium TA
98 ve TA 100 suşları kullanıldı. Elde edilen verilere göre gilaburu
meyvelerinin ağırlığı ortalama olarak 0.80 g, suda çözünür kuru madde (SÇKM)
içeriği % 11.3, pH’sı 3.20, toplam antosiyanin miktarı 0.80
mg/g,1,1-diphenyl-2-picrylhydrazyl (DPPH)radikal süpürücü etkisi % 52.5,
2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) değeri 72.8 µmol
TE/g FW, Ferric Reducing Antioxidant Power (FRAP) değeri ise 122.6 µmol Fe II/g
FW olarak belirlendi. Ayrıca gilaburu ekstraktının test mikroorganizmaları
üzerinde değişen oranlarda antibakteriyel aktiviteye sahip oldukları; ancak
antifungal aktivite sergilemedikleri belirlendi. Test bakterileri içerisinde
ise en duyarlı olanının Pseudomonas aeruginosa ATCC 9027 olduğu belirlendi.
Antimutajenite deneylerinde,TA98 suşlarında gilaburunun sadece 40 µl dozunda
pozitif kontrole göre önemli bir azalma meydana getirdi. TA100 suşlarında ise
hiçbir dozda pozitif kontrole göre istatistiksel olarak önemli bir azalma
meydana gelmediği belirlendi.

Keywords

Antimikrobiyal, Antimutajen, Antioksidan, Gilaburu, Viburnum opulus

References

• Ahmad, N. and Mukhtar, H., 1999. Green tea polyphenols and cancer: Biologic mechanisms and practical implications, Nutrition Reviews, 57, 3, 78-83.
• Altun, M.L., Çıtoğlu, G.S., Yılmaz, B.S. and Çoban, T., 2008. Antioxidant properties of Viburnum opulus and Viburnum lantanagrowing in Turkey, International Journal of Food Sciences and Nutrition, 59, 3, 175-180.
• Azirak, S., and Rencuzogullari, E., 2008. The in vivo genotoxic effects of carvacrol and thymol in rat bone marrow cells, Environmental Toxicology, 23, 6, 728-735.
• Bakhshi, D. and Arakawa, O., 2006. Effects of UV-b irradiation on phenolic compound accumulation and antioxidant activity in ‘Jonathan’ apple influenced by bagging, temperature and maturation, Journal of Food, Agriculture & Environment, 4, 1, 75-79.
• Benzie, I.F.F. and Strain, J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant power; The FRAP assay, Analytical Biochemistry, 239, 70-76.
• Bolat, S.ve Özcan, M.,1995. Gilaburu (Viburnum opulus L.) Meyvesinin Morfolojik, Fenolojik ve Pomolojik Özellikleri ile Kimyasal Bileşimi. Türkiye II. Ulusal Bahçe Bitkileri Kongresi, Ç.Ü. Ziraat Fak. Yay, Cilt I., Adana. 72s.
• Bubulica, M.V., Anghel, I., Grumezescu, A.M., Saviuc, C., Anghel, G.A., Chifiriuc, M.C., Gheorghe, I., Lazar, V., and Popescu, A., 2012. In vitro evaluation of bactericidal and antibio film activity of Loniceratatarica and Viburnum opulus plant extractson Staphylococcus Strains, Farmacia, 60, 1, 80-91.
• Burnaz, N., 2007. Viburnum opulus ve V. orientalebitki ekstraktlarının kimyasal bileşimi ve biyolojik aktiviteleri, Yüksek Lisans Tezi, Karadeniz Teknik Üniversitesi Fen Bilimleri Enstitüsü, Trabzon. 66s.
• Buyukleyla, M, and Rencuzogullari, E., 2008. The effects of thymol on sister chromatid Exchange, chromosome aberration and micronucleus in human lymphocytes, Ecotoxicology Environmental Safety, Doi:10.1016/j.ecoenv.2008.10.005.
• Cemeroğlu, B., 1992. Meyve ve sebze işleme endüstrisinde temel analiz metotları. Ankara: Biltav Yayınları.
• Česonienė, L., Daubaras, R., Venclovienė, J. and Viškelis, P., 2010. Biochemical and agro-biological diversity of Viburnum opulus genotypes, Central European Journal of Biology, 5, 6, 864–871.
• Česoniene, L., Daubaras, R., Viškelis, P. and Šarkinas, A., 2012. Determination of the total phenolic and anthocyanin contents and antimicrobial activity of Viburnum Opulus fruit juice, Plant Foods for Human Nutrition, 67, 3, 256-261.
• Česoniene, L., Daubaras, R., Kraujalyte, V., Venskutonis, P.R. and Šarkinas, A., 2014. Antimicrobial activity of Viburnum opulus fruit juices and extracts, Journal fur Verbraucherschutz und Lebensmittelsicherheit, 9, 2, 129-132.
• Eryilmaz, M., Ozbilgin, S., Ergene, B., Sever Yilmaz, B., Altun, M.L. and Saltan, G., 2013. Antimicrobial activity of Turkish Viburnum species, Bangladesh Journal of Botany, 42, 2, 355-360.
• Franzios, G., Mirotsou, M., Hatziapostolou, E., Kral, J., Scouras, Z.G., and Mavragani, P.T., 1997. Insecticidal and genotoxic activities of mint essential oils, Journal of Agricultural and Food Chemistry, 45, 2690-2694.
• Giusti, M.M. and Wrolstad, R.E., 2001. Anthocyanins characterization and measurement with UV visible spectroscopy, Current protocols in food analytical chemistry. Editor: Wrolstad, R. E. New York: Willey.
• Hızlısoy, H., 2009. Çeşitli mikroorganizmalar üzerine gilaburunun antimikrobiyal etkisinin incelenmesi. Erciyes Üniversitesi, Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi.
• Ipek, E., Tuylu, B.A., and Zeytinoğlu, H., 2003. Effects of carvacrol on sister chromatid exchances in human lymphocyte cultures, Cytotechnology, 43, 145-148.
• İskender, N.Y., 2007. Türkiye Doğal Viburnum L. (Caprıfolıaceae) türlerinin uçucu yağ bileşimleri ve antimikrobiyal aktiviteleri, Yüksek Lisans Tezi, Karadeniz Teknik Üniversitesi Fen Bilimleri Enstitüsü.
• Koliopoulos, G., Pitarokili, D., Kioulos, E., Michaelakis, A. and Tzakou, O., 2010. Chemical composition and larvicidal evaluation of Mentha, Salvia, and Melissa essential oils against the West Nile virus mosquito Culex pipiens, Parasitol Research, 107, 2, 327-35.
• Kraujalytė, V., Venskutonis, P.R., Pukalskas, A., Česonienė, L. and Daubaras, R., 2013. Antioxidant properties and polyphenolic compositions of fruits from different European cranberrybush (Viburnum opulusL.) genotypes, Food Chemistry, 141, 3695–3702.
• Maron, D.M. and Ames, B.N., 1983. Revised methods fort he Salmonella mutagenicity test, Mutation Research, 113, 173-215.
• Mennen, L.I., Walker, R., Bennetau-Pelissero, C. and Scalbert, A., 2005. Risks and safety of polyphenol consumption, The American Journal of Clinical Nutrition, 81, 326–329.
• Orakçı, E.E., 2010. Gilaburunun antioksidan aktivitesi. Erciyes Üniversitesi Eczacılık Fakültesi, Bitirme tezi.
• Özbek, S., 1977. Genel Meyvecilik. Çukurova Üniv. Ziraat Fak. Yayınları 111, Ders Kitabı 6.
• Özcan, H.M., 2010. Fenolik bileşiklerin tayinine yönelik amperometrik esaslı biyosensör hazırlanması. Trakya Üniversitesi FenEdebiyat Fakültesi Kimya bölümü. Doktora tezi.
• Özçelik, S., 1992. Gıda Mikrobiyolojisi Laboratuar Kılavuzu. F.Ü. Fen-Edebiyat Fakültesi Yayınları, Yayın No: 1, Elazığ, 135. Papageorgiou, V., Gardeli, C., Mallouchos, A., Papaioannou, M. and Komaitis, M., 2008. Variation of the chemical profile and antioxidant behavior of Rosmarinus officinalis L. and Salvia fruticosa Miller grown in Greece, Journal of Agricultural and Food Chemistry, 56, 7254-7264.
• Paulauskas, A., Žukauskienė, J., Žiaukienė, D. Česonienė, L., Daubaras, R., Kupčinskienė, E.,Lazutka, J.R., Slapšytė, G., Dedonytė, V., Mierauskienė, J., Stapulionytė, A., Paškevičius, A., Levinskaitė, L., Švedienė J. and Viškelis, P., 2015. Differentiation of Viburnum accessions according to their molecular, biochemical, genotoxic and microbiological features of importance to selection, Academia Journal of Agricultural Research, 3, 6, 081-093.
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical Biology and Medicine, 26, 1231-1237.
• Rezaeirad, D., Bakhshi, D., Ghasemnezhad, M. and Lahiji, H.S., 2013. Evaluation of some quantitative and qualitative characteristics of local pears (Pyrus sp.) in the North of Iran, International Journal of Agriculture and Crop Sciences, 5, 8, 882-887.
• Sagdic, O., Aksoy, A. and Ozkan, G., 2006. Evaluation of the antibacterial and antioxidant potentials of cranberry (gilaburu, Viburnum opulus L.) fruit extract, Acta Alimentaria, 35, 4, 487-492.
• Sağıroğlu, A., 2003. Bitkisel doğal bileşikler kimyası, Trakya Üniversitesi FenEdebiyat Fakültesi Kimya bölümü biyokimya anabilim dalı, 12-13.
• Stammati, A., Bonsi, P., Zucco, F., Moezelaar, R., Alakomi, H.L., and Von Wright, A., 1999. Toxicity of selected plant volatiles in microbial and mammalian short-term assays, Food Chemistry Toxicology, 37, 813-823.
• Şeker, I.T. Ertop, M.H. and Hayta, M., 2016. Physicochemical and bioactive properties of cakes incorporated with gilaburu fruit (Viburnum opulus) pomace, Quality Assurance and Safety of Crops and Foods, 8, 2, 261-266. Yilmaz, N., Yali, N., Misir, G., Coskuncelebi, K., Karaoglu, S. and Yayli, N., 2008. Chemical composition and antimicrobial activities of the essential oils of Viburnum opulus, Viburnum lantana and Viburnum orientala, Asian Journal of Chemistry, 20, 3324–3330.
• Yürüker, A., 1993. Viburnum orientale Pallas üzerinde fitokimyasal çalışmalar, Doktora Tezi, Hacettepe Üniversitesi Sağlık Bilimleri Enstitüsü, Ankara, 5-15.
• Wan-Ibrahim, W.I., Sidik, K. and Kuppusamy, U.R., 2010. A high antioxidant level in edible plants is associated with genotoxic properties, Food Chemistry, 122, 4, 1139-1144.