Clinopodium vulgare L. subsp. vulgare Ekstresinin Antioksidan, Antimikrobial, Tirozinaz İnhibitor Aktiviteleri ve RP-HPLC ile Fenolik Bileşiklerinin Araştırılması

Year 2018, Volume: 8 Issue: 2, 230 - 238, 31.07.2018

Rezzan Aliyazıcıoğlu , Sıla Özlem Şener , Nuriye Korkmaz , Şeyda Akkaya Ufuk Özgen Şengül Alpay Karaoğlu

https://doi.org/10.17714/gumusfenbil.306925

Abstract

Clinopodium vulgare L. subsp. vulgare, Clinopodium
cinsine ve Lamiaceae familyasına mensup çok yıllık aromatik otsu bir bitkidir.
Bu cins bitkiler sıklıkla geleneksel tedavide kullanılmaktadır. Farklı
Clinopodium türlerinin farklı ekstrelerinin antioksidan, antimikrobiyal,
tirozinaz inhibitör etkilerini ve HPLC ile fenolik bileşenlerinin analizini
konu alan çalışmalar olmasına rağmen, Clinopodium vulgare L. subsp. vulgare ekstresinin
bu özelliklerinin tümünü içeren herhangi bir çalışma bulunmamaktadır. Bu
çalışmanın amacı C. vulgare  subsp.
vulgare metanol ekstresinin antioksidan, antimikrobiyal, tirozinaz inhibitör
aktivitelerini ve HPLC analizi ile fenolik madde içeriğini belirlemektir. Ekstrenin fenolik
bileşenleri ters faz yüksek performanslı sıvı kromatografisi (RP-HPLC) ile
belirlendi. Antioksidan, tirozinaz inhibitör aktivite spektrofotometrik
yöntemlerle ve antimikrobiyal aktivite disk difüzyon metodu ile incelendi. Ekstrenin toplam fenolik madde miktarı 27.9±0.4 mg gallik
asit eşdeğeri/g numune, 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürme kapasitesi
IC50 değeri 0.114±0.0004 mg/mL ve ferrik indirgeyici antioksidan güç
(FRAP) değeri 1556±3 µM troloks eşdeğeri/g numune olarak hesaplandı. Tirozinaz
inhibisyon çalışma sonucuna göre ekstrenin IC50 değeri kojik asit
standardından yüksek bulundu. HPLC ile analiz sonucunda protokatekuik asit,
klorojenik asit, vanilin, sinapik asit ve benzoik asit tespit edildi. Ekstre,
asid-hızlı bakteri (M. smegmatis), bazı gram pozitif (S. aureus ve B. cereus)
ve bazı gram negatif (Y. pseudotuberculosis) bakterilere karşı ılımlı
antibakteriyal aktivite gösterdi. Ancak C. albicans and S. cerevisiae türlerine
karşı hiçbir antifungal aktivite göstermedi. Bu çalışmanın sonuçlarına göre, C. vulgare subsp. vulgare yeni
farmasotiklerin geliştirilmesinde potansiyel bir kaynak olarak düşünülebilir. 

Keywords

Antioksidan, Antimikrobiyal, Tirozinaz İnhibitör Aktivite, HPLC

Investigation of Phenolic Compounds by RP-HPLC and Antioxidant, Antimicrobial, Tyrosinase Inhibitor Activities of Clinopodium vulgare L. subsp. vulgare Extract

Abstract

Clinopodium vulgare L. subsp. vulgare is a perennial
aromatic herbaceous plant belonging to Clinopodium genus and Lamiaceae family.
This genus plants are often used in traditional therapy. There are no studies
involving the whole of these properties of the Clinopodium vulgare L. subsp.
vulgare extract, although there are studies about antioxidant, antimicrobial,
tyrosinase inhibitor effects and analysis of phenolic compounds by HPLC of
different extracts of different Clinopodium genus. The purpose of this study
was to determine the phenolic composition by HPLC, and antioxidant,
antimicrobial, and tyrosinase inhibitor activity of methanolic extract of C. vulgare  subsp. vulgare. The phenolic compounds were determined by reverse
phase high performance liquid chromatography (RP-HPLC). The antioxidant, tyrosinase
inhibitor, and antimicrobial activities of the extract were examined by spectrophotometric
methods, and disc diffusion method, respectively. The total phenolic content
(TPC), 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), and
ferric reducing antioxidant power (FRAP) values of the extract were found
27.9±0.4 mg gallic acid equivalents per g sample, 0.114±0.0004 mg/mL, and 1556±3
µM trolox equivalents per g sample, respectively. IC50 value of the extract according to the
tyrosinase inhibitition study results was found higher than kojic acid standard.
The protocatechuic acid, chlorogenic acid, vanillin, sinapic acid, benzoic acid
were detected by HPLC analysis. The extract exhibited while moderate
antibacterial activity against an acid-fast bacterium (M. smegmatis), some gram
pozitive (S. aureus and B. cereus) and gram negative (Y. pseudotuberculosis)
bacteria. But, antifungal activity was not showed against C. albicans and S. cerevisiae species. According to
the results of this study, C. vulgare  subsp. vulgare can be
considered as a potential source for developing new pharmaceuticals.

Keywords

Antioxidants, Antimicrobial, Tyrosinase inhibition, HPLC

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