Kadmiyum ve Kurşunun Achroia grisella Fabr. (Lepidoptera: Pyralidae)’nın Toplam Hemosit Sayısına Etkisi
Year 2020,
Volume: 10 Issue: 1, 190 - 194, 15.01.2020
Nevran Eylem Akman Gündüz
,
Sevcan Mercan
,
Özgür Özcan
Abstract
Kadmiyum (Cd) ve kurşunun (Pb) Achroia grisella’nın toplam hemosit sayısı üzerindeki etkisi laboratuar koşullarında araştırılmıştır. Larvalar üç farklı konsantrasyonda (50, 100 and 200 mg/kg) Cd ve Pb ile kontamine olmuş yapay besin ortamında yetiştirilmiştir. Kontrol grubunda böcekler ağır metal içermeyen besin ile beslenmiştir. Tüm böcekler 25 ± 2 °C sıcaklık, 60 ± 5% bağıl nem (RH) ve 16A:8K fotoperiyot koşullarında tutulmuştur. Elde edilen bulgular kadmiyuma maruz kalan larvaların toplam hemosit sayısının tüm konsantrasyonlarda azaldığını, kurşuna maruz kalanlarda ise toplam hemosit sayısının sadece en yüksek konsantrasyonda azaldığını göstermiştir.
References
- Ahmad, A. and Khan, M.A., 1988. Effects of triol and makisterone A on the hemocytes of Hieroglymphus nigrorepletus Boliver (Orthoptera: Acrididae). Proceedings of the Indian Academy of Sciences (Animal Sciences), 97(3), 203-210.
- Ahmad, A., 1995. Changes in hemocyte counts following topical application of β-ecdysone and makisterone A on 5th instar nymphs of Dysdercus cingulatus Fabr. (Hemiptera: Pyrrhocoridae). Entomologia Croatica, 1, 41-48.
- Altuntaş, H., Kılıç, A.Y.¸ Uçkan, F. and Ergin, E., 2012. Effects of gibberellic acid on hemocytes of Galleria mellonella L. (Lepidoptera: Pyralidae). Environmental Entomology, 41(3), 688-696.
- Banakou, E. and Dailianis, S., 2010. Involvement of Na+/H+ exchanger and respiratory burst enzymes NDPH oxidase and NO synthase, in Cd-induced lipid peroxidation and DNA damage in haemocytes of mussels. Comparative Biochemistry and Physiology - Part C, 152, 346–352.
- Berger, J. and Slavičková, K., 2008. Morphological characterization of hemocytes in the adult linden bug, Pyrrhocoris apterus (L.) (Heteroptera). Zoological Studies, 47(4), 466-472.
- Bischof, C., 1995. Effects of heavy metal stress on carbohydrate and lipid concentrations in the haemolymph and total body tissue of parasitized Lymantria dispar L. larv; (Lepidoptera). Comparative Biochemistry and Physiolology – Part C, 112(1), 87-92.
- Borowska, J., Sulima, B., Niklińska, M. and Pyza E., 2004. Heavy metal accumulation and its effects on development, survival and immuno-competent cells of the house fly Musca domestica from closed laboratory populations as model organism. Fresenius Environmental Bulletin, 13, 1402-1409.
- Borowska, J. and Pyza, E., 2011. Effects of heavy metals on insect immunocompetent cells. Journal of Insect Physiology, 57, 760-770.
- Çelik, D., Özbek, R., Uçkan, F., 2017. Effects of indole-3-acetic acid on hemocytes of Achoria grisella Fabr. (Lepidoptera: Pyralidae). Journal of Entomological Research Society, 19(2), 83-93.
- Ellis, J.D., Graham, J.R. and Mortensen, A., 2013. Standard methods for wax moth research. Journal of Apicultural Research, 52(1), 1-17.
- El-Sheikh, T.M.Y., Fouda, M.A., Hassan, M.I., Abd-Elhamed, A. Abd-Elghaphar and Hasaballah, A.I., 2010. Toxicological effects of some heavy metal ions on Culex pipiens L. (Diptera: Culicidae). Egyptian Academic Journal of Biological Sciences, 2(1), 63-76.
- Eslin, P. and Prévost, G., 1998. Hemocyte load and immune resistance to Asobora tabida are correlated in species of the Drosophila melanogaster subgroup. Journal of Insect Physiology, 44, 807-816.
- Fitts, J. E. and Laird, D., 2004. Standart methods for the examination of dairy products:direct microscobic methods for bacteria of somatic cells (Chapter 10). Wehl, H. M., Frank, J. F., American Public Health Assoc.
- Gleason J.M., Zhou, Y., Hackett, J.L., Haris, B.R. and Greenfield, M.D., 2016. Development of a genomic resource and quantitative trait loci mapping of male calling traits in the lesser wax moth, Achroia grisella. PLoS ONE 11(1): e0147014. doi:10.1371/ journal.pone.0147014.
- İzzetoğlu, S., Karaçali, S., 2003. The effects of 20-hydroxyecdysone on hemocytes of Galleria mellonella (Lepidoptera) in vitro conditions. Gazi University, Journal of Science, 16, 233-238.
- Kazimírová, M. and Slovák, M., 1996. Effects of heavy metals and fluorine on phagocytosis and phenoloxidase activity in Mamestra brassicae (Lepidoptera: Noctuidae). European Journal of Entomology, 93, 467-473.
- Kul, E., 2013. Jersey sığırlarında süt insülin benzeri büyüme faktörü-I (Igf-I) konsantrasyonu ile doğrusal puanlama özellikleri, süt somatik hücre sayısı ve verim özellikleri arasındaki ilişkiler, Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi, Samsun.
- Kurt, D., Kayış, T., 2015. Effects of the pyrethroid insecticide deltamethrin on the hemocytes of Galleria mellonella. Turkish Journal of Zoology, 39, 452-457.
- Mahgoup, M.O, Lau, W.H. and Bin Omar, D., 2015. Observations on the biology and larval instars discrimination of wax moth Achroia grisella F. (Pyralidae: Lepidoptera). Journal of Entomology, 12(1), 1-11.
- Prasada Rao, C.G., Ray, A. Ramamurty, P.S. 1984. Effect of ligation and ecdysone on total haemocyte count in the tobacco caterpillar, Spodoptera litura (Noctuidae:Lepidoptera). Canadian Journal of Zoology, 62, 1461-1463.
- Sanjayan, K.P., Ravikumar, T. and Albert, S., 1996. Changes in the haemocyte profile of Spilostethus hospes (Fab) (Heteroptera: Lygaeidae) in relation to eclosion, sex and mating. Journal of Biosciences, 21(6), 781-788.
- Sendi, J.J. and Salehi, R., 2010. The effect of methoprene on total hemocyte counts and histopathology of hemocytes in Papilio demoleus L. (Lepidoptera). Munis Entomology & Zoology, 5(1), 240-246.
- Sezer, B. and Özalp, P., 2015. Effects of pyriproxyfen on hemocyte count and morphology of Galleria mellonella. Fresenius Environmental Bulletin, 24(2a), 621-625.
- Sharma, P.R., Sharma, O.P. and Saxena, B.P., 2003. Effect of neem gold on hemocytes of the tobacco armyworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Current Science, 84, 690-695.
- Siddiqui, M.I. and Al-Khalifa, M.S., 2014. Review of haemocyte count, response to chemicals, phagocytosis, encapsulation and metamorphosis in insects. Italian Journal of Zoology, 81(1), 2-15.
- Suganya, M., Karthi, S. and Shivakumar, M.S., 2016. Effect of cadmium and lead exposure on tissue specific antioxidant response in Spodoptera litura. Free Radicals and Antioxidants, 6(1), 90-100.
Effect of Cadmium and Lead on Total Hemocyte Count of Achroia grisella Fabr. (Lepidoptera: Pyralidae)
Year 2020,
Volume: 10 Issue: 1, 190 - 194, 15.01.2020
Nevran Eylem Akman Gündüz
,
Sevcan Mercan
,
Özgür Özcan
Abstract
The effect of cadmium (Cd) and lead (Pb) on total hemocyte count (THC) of Achroia grisella was examined under laboratory conditions. Larvae were reared on an artificial diet medium contaminated with three different concentrations (50, 100 and 200 mg/kg) of Cd and Pb. In the control group the insects fed a heavy metal free diet. All of the insects were kept at 25 ± 2 °C temperature, 60 ± 5% relative humidity and 16L:8D photoperiod conditions.The obtained data showed that the total hemocyte count of larvae exposed to Cd resulted in a significant decrease at all concentrations, whereas total hemocyte count of Pb-treated groups decreased only at the highest concentrations of Pb.
References
- Ahmad, A. and Khan, M.A., 1988. Effects of triol and makisterone A on the hemocytes of Hieroglymphus nigrorepletus Boliver (Orthoptera: Acrididae). Proceedings of the Indian Academy of Sciences (Animal Sciences), 97(3), 203-210.
- Ahmad, A., 1995. Changes in hemocyte counts following topical application of β-ecdysone and makisterone A on 5th instar nymphs of Dysdercus cingulatus Fabr. (Hemiptera: Pyrrhocoridae). Entomologia Croatica, 1, 41-48.
- Altuntaş, H., Kılıç, A.Y.¸ Uçkan, F. and Ergin, E., 2012. Effects of gibberellic acid on hemocytes of Galleria mellonella L. (Lepidoptera: Pyralidae). Environmental Entomology, 41(3), 688-696.
- Banakou, E. and Dailianis, S., 2010. Involvement of Na+/H+ exchanger and respiratory burst enzymes NDPH oxidase and NO synthase, in Cd-induced lipid peroxidation and DNA damage in haemocytes of mussels. Comparative Biochemistry and Physiology - Part C, 152, 346–352.
- Berger, J. and Slavičková, K., 2008. Morphological characterization of hemocytes in the adult linden bug, Pyrrhocoris apterus (L.) (Heteroptera). Zoological Studies, 47(4), 466-472.
- Bischof, C., 1995. Effects of heavy metal stress on carbohydrate and lipid concentrations in the haemolymph and total body tissue of parasitized Lymantria dispar L. larv; (Lepidoptera). Comparative Biochemistry and Physiolology – Part C, 112(1), 87-92.
- Borowska, J., Sulima, B., Niklińska, M. and Pyza E., 2004. Heavy metal accumulation and its effects on development, survival and immuno-competent cells of the house fly Musca domestica from closed laboratory populations as model organism. Fresenius Environmental Bulletin, 13, 1402-1409.
- Borowska, J. and Pyza, E., 2011. Effects of heavy metals on insect immunocompetent cells. Journal of Insect Physiology, 57, 760-770.
- Çelik, D., Özbek, R., Uçkan, F., 2017. Effects of indole-3-acetic acid on hemocytes of Achoria grisella Fabr. (Lepidoptera: Pyralidae). Journal of Entomological Research Society, 19(2), 83-93.
- Ellis, J.D., Graham, J.R. and Mortensen, A., 2013. Standard methods for wax moth research. Journal of Apicultural Research, 52(1), 1-17.
- El-Sheikh, T.M.Y., Fouda, M.A., Hassan, M.I., Abd-Elhamed, A. Abd-Elghaphar and Hasaballah, A.I., 2010. Toxicological effects of some heavy metal ions on Culex pipiens L. (Diptera: Culicidae). Egyptian Academic Journal of Biological Sciences, 2(1), 63-76.
- Eslin, P. and Prévost, G., 1998. Hemocyte load and immune resistance to Asobora tabida are correlated in species of the Drosophila melanogaster subgroup. Journal of Insect Physiology, 44, 807-816.
- Fitts, J. E. and Laird, D., 2004. Standart methods for the examination of dairy products:direct microscobic methods for bacteria of somatic cells (Chapter 10). Wehl, H. M., Frank, J. F., American Public Health Assoc.
- Gleason J.M., Zhou, Y., Hackett, J.L., Haris, B.R. and Greenfield, M.D., 2016. Development of a genomic resource and quantitative trait loci mapping of male calling traits in the lesser wax moth, Achroia grisella. PLoS ONE 11(1): e0147014. doi:10.1371/ journal.pone.0147014.
- İzzetoğlu, S., Karaçali, S., 2003. The effects of 20-hydroxyecdysone on hemocytes of Galleria mellonella (Lepidoptera) in vitro conditions. Gazi University, Journal of Science, 16, 233-238.
- Kazimírová, M. and Slovák, M., 1996. Effects of heavy metals and fluorine on phagocytosis and phenoloxidase activity in Mamestra brassicae (Lepidoptera: Noctuidae). European Journal of Entomology, 93, 467-473.
- Kul, E., 2013. Jersey sığırlarında süt insülin benzeri büyüme faktörü-I (Igf-I) konsantrasyonu ile doğrusal puanlama özellikleri, süt somatik hücre sayısı ve verim özellikleri arasındaki ilişkiler, Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi, Samsun.
- Kurt, D., Kayış, T., 2015. Effects of the pyrethroid insecticide deltamethrin on the hemocytes of Galleria mellonella. Turkish Journal of Zoology, 39, 452-457.
- Mahgoup, M.O, Lau, W.H. and Bin Omar, D., 2015. Observations on the biology and larval instars discrimination of wax moth Achroia grisella F. (Pyralidae: Lepidoptera). Journal of Entomology, 12(1), 1-11.
- Prasada Rao, C.G., Ray, A. Ramamurty, P.S. 1984. Effect of ligation and ecdysone on total haemocyte count in the tobacco caterpillar, Spodoptera litura (Noctuidae:Lepidoptera). Canadian Journal of Zoology, 62, 1461-1463.
- Sanjayan, K.P., Ravikumar, T. and Albert, S., 1996. Changes in the haemocyte profile of Spilostethus hospes (Fab) (Heteroptera: Lygaeidae) in relation to eclosion, sex and mating. Journal of Biosciences, 21(6), 781-788.
- Sendi, J.J. and Salehi, R., 2010. The effect of methoprene on total hemocyte counts and histopathology of hemocytes in Papilio demoleus L. (Lepidoptera). Munis Entomology & Zoology, 5(1), 240-246.
- Sezer, B. and Özalp, P., 2015. Effects of pyriproxyfen on hemocyte count and morphology of Galleria mellonella. Fresenius Environmental Bulletin, 24(2a), 621-625.
- Sharma, P.R., Sharma, O.P. and Saxena, B.P., 2003. Effect of neem gold on hemocytes of the tobacco armyworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Current Science, 84, 690-695.
- Siddiqui, M.I. and Al-Khalifa, M.S., 2014. Review of haemocyte count, response to chemicals, phagocytosis, encapsulation and metamorphosis in insects. Italian Journal of Zoology, 81(1), 2-15.
- Suganya, M., Karthi, S. and Shivakumar, M.S., 2016. Effect of cadmium and lead exposure on tissue specific antioxidant response in Spodoptera litura. Free Radicals and Antioxidants, 6(1), 90-100.