HeLa ve Beas-2B Hücre Hatlarına Karşı Benzimidazolyum Tuzlarının İn Vitro Sitotoksik Aktivite Çalışmaları

Year 2020, Volume: 10 Issue: 2, 505 - 513, 15.04.2020

Senem Akkoc

https://doi.org/10.17714/gumusfenbil.613631

Abstract

1H-benzo[d]imidazol çekirdeği içeren altı farklı heterosiklik bileşik (S1-S6) iki basamakta hazırlandı ve
yapıları farklı spektroskopik yöntemler kullanılarak karakterize edildi. Sentezlenen
heterosiklik bileşikler insan serviks adenokarsinom (HeLa) ve insan sağlıklı
akciğer (Beas-2B) hücre hatlarına karşı test edildi. Pozitif kontrol ilaç
olarak cisplatin aynı şartlar altında serviks hücre hattına karşı denendi.
Bileşik S4'ün HeLa hücre hattına karşı yüksek sitotoksik aktivite gösterdiği (IC₅₀:
3.72 ± 0.14 µM) bulundu.
Çalışmanın son basamağında, ilaç adayları ile etkileştirilen hücrelerin renkli
görüntüleri konfokal mikroskobu kullanılarak alındı. 

Keywords

Benzimidazol, Heterosiklik Bileşik, Konfokal, Sitotoksik Aktivite

In Vitro Cytotoxic Activity Studies of Benzimidazolium Salts against HeLa and Beas-2B Cell Lines

Abstract

Six different heterocyclic
compounds containing 1H-benzo[d]imidazole nuclei (S1-S6) were prepared in two steps and their structures were characterized
using different spectroscopic methods. The synthesized heterocyclic compounds
were tested against human cervix adenocarcinoma (HeLa) and human healthy lung
(Beas-2B) cell lines. Cisplatin as a positive control drug was tested against the cervix
cell line under the same conditions. Compound S4 was found to exhibit high cytotoxic activity against HeLa cell
line (IC₅₀: 3.72 ± 0.14 µM). In the last step of the study, color
images of the cells interacted with the drug candidates were taken using confocal
microscope.

Keywords

Benzimidazole, Heterocyclic Compound, Confocal, Cytotoxic Activity

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