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Cytotoxicity of silver nanoparticles obtained from Eruca vesicaria on rainbow trout gonad cell line-2 (RTG-2)

Year 2022, Volume: 12 Issue: 4, 1093 - 1101, 15.10.2022
https://doi.org/10.17714/gumusfenbil.1104370

Abstract

The rising application of silver (Ag) nanoparticles in many sectors such as food, medicine, and agriculture lead to toxic effects on the ecological environment. Thus, studies on biological synthesis methods are carried out in order to diminish the toxicity caused by Ag nanoparticle synthesis methods. However, studies on the toxicity of biosynthesized Ag nanoparticles on fish cell lines are very few. The purpose of this research was to perform the biological synthesis of Ag nanoparticles via Eruca vesicaria plant extract and to examine their toxicity in rainbow trout gonad cell line-2 (RTG 2). The characterization of Ag nanoparticles obtained from E. vesicaria was done by UV-vis, TEM, and XRD. The toxicity of Ag nanoparticles (100 µg/mL- 6,25 µg/mL) in the RTG-2 cell for 24 hours was determined by sulforhodamine B assay. Ag nanoparticles obtained in the form of a sphere, triangle, cube, and sizes of 5-20 nm showed significant a toxic effect on RTG-2 fish cells depending on the dose at p≤0,001 levels. This study is important in terms of proving that Ag nanoparticles obtained by biological synthesis have a toxic effect on fish cell lines and showing that there is a need for studies to reduce the release of Ag nanoparticles to the environment rather than synthesis methods.

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Gökkuşağı alabalığı gonad hücre hattı-2 (RTG-2) üzerinde Eruca vesicaria'dan elde edilen gümüş nanoparçacıkların sitotoksisitesi

Year 2022, Volume: 12 Issue: 4, 1093 - 1101, 15.10.2022
https://doi.org/10.17714/gumusfenbil.1104370

Abstract

Gümüş (Ag) nanoparçacıklarının gıda, ilaç, tarım gibi birçok sektörde artan kullanımı ekolojik çevre üzerinde toksik etkilere yol açmaktadır. Bu nedenle Ag nanoparçacık sentez yöntemlerinin neden olduğu toksisiteyi azaltmak için biyolojik sentez yöntemleri üzerinde çalışmalar yapılmaktadır. Bununla birlikte, balık hücre hatlarında biyosentezlenmiş Ag nanoparçacıklarının toksisitesi üzerine yapılan çalışmalar çok azdır. Bu çalışmanın amacı, Eruca vesicaria bitki özü ile Ag nanoparçacıklarının biyolojik sentezini gerçekleştirmek ve gökkuşağı alabalığı gonad hücre hattı-2'de (RTG 2) toksisitelerini incelemektir. E. vesicaria'dan elde edilen Ag nanoparçacıklarının karakterizasyonu UV-vis, TEM ve XRD ile yapılmıştır. Ag nanoparçacıklarının (100 µg/mL- 6,25 µg/mL) 24 saat boyunca RTG-2 hücresindeki toksisitesi sülforodamin B tahlili ile belirlendi. Küre, üçgen, küp ve 5-20 nm boyutlarında elde edilen Ag nanopartiküller, p≤0,001 seviyelerinde doza bağlı olarak RTG-2 balık hücreleri üzerinde önemli toksik etki göstermiştir. Bu çalışma, biyolojik sentez yoluyla elde edilen Ag nanoparçacıklarının balık hücre hatları üzerinde toksik etkisinin olduğunun kanıtlanması ve sentez yöntemlerinden ziyade Ag nanoparçacıklarının çevreye salınımını azaltacak çalışmalara ihtiyaç olduğunu göstermesi açısından önemlidir.

References

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  • Alyousef, A. A., Arshad, M., AlAkeel, R., & Alqasim, A. (2019). Biogenic silver nanoparticles by Myrtus communis plant extract: biosynthesis, characterization and antibacterial activity. Biotechnology & Biotechnological Equipment, 33(1), 931-936. https://doi.org/10.1080/13102818.2019.1629840
  • Aritonang, H. F., Koleangan, H., & Wuntu, A. D. (2019). Synthesis of silver nanoparticles using aqueous extract of medicinal plants’(Impatiens balsamina and Lantana camara) fresh leaves and analysis of antimicrobial activity. International journal of microbiology, 2019. https://doi.org/10.1155/2019/8642303
  • Baghizadeh, A., Ranjbar, S., Gupta, V. K., Asif, M., Pourseyedi, S., Karimi, M. J., & Mohammadinejad, R. (2015). Green synthesis of silver nanoparticles using seed extract of Calendula officinalis in liquid phase. Journal of molecular liquids, 207, 159-163. https://doi.org/10.1016/j.molliq.2015.03.029
  • Balachandar, R., Gurumoorthy, P., Karmegam, N., Barabadi, H., Subbaiya, R., Anand, K., ... & Saravanan, M. (2019). Plant-mediated synthesis, characterization and bactericidal potential of emerging silver nanoparticles using stem extract of Phyllanthus pinnatus: a recent advance in phytonanotechnology. Journal of Cluster Science, 30(6), 1481-1488. https://doi.org/10.1007/s10876-019-01591-y
  • Baran, A., Baran, M. F., Keskin, C., Kandemir, S. I., Valiyeva, M., Mehraliyeva, S., ... & Eftekhari, A. (2021). Ecofriendly/Rapid synthesis of silver nanoparticles using extract of waste parts of artichoke (cynara scolymus L.) and evaluation of their cytotoxic and antibacterial activities. Journal of Nanomaterials, 2021. https://doi.org/10.1155/2021/2270472
  • Baran, A., Fırat Baran, M., Keskin, C., Hatipoğlu, A., Yavuz, Ö., İrtegün Kandemir, S., ... & Eftekhari, A. (2022). Investigation of antimicrobial and cytotoxic properties and specification of silver nanoparticles (AgNPs) derived from Cicer arietinum L. green leaf extract. Frontiers in Bioengineering and Biotechnology, 263. https://doi.org/10.3389/fbioe.2022.855136
  • Behravan, M., Panahi, A. H., Naghizadeh, A., Ziaee, M., Mahdavi, R., & Mirzapour, A. (2019). Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International journal of biological macromolecules, 124, 148-154. https://doi.org/10.1016/j.ijbiomac.2018.11.101
  • Bélteky, P., Rónavári, A., Igaz, N., Szerencsés, B., Tóth, I. Y., Pfeiffer, I., ... & Kónya, Z. (2019). Silver nanoparticles: Aggregation behavior in biorelevant conditions and its impact on biological activity. International journal of nanomedicine, 14, 667. https://doi.org/10.2147/IJN.S185965
  • Bermejo-Nogales, A., Fernández-Cruz, M. L., & Navas, J. M. (2017). Fish cell lines as a tool for the ecotoxicity assessment and ranking of engineered nanomaterials. Regulatory Toxicology and Pharmacology, 90, 297-307. https://doi.org/10.1016/j.yrtph.2017.09.029
  • Caminada D, Escher C & Fent, K. (2006). Cytotoxicity of pharmaceuticals found in aquatic systems: Comparison of PLHC-1 and RTG-2 fish cell lines. Aquatic Toxicology, 79(2), 114–123. https://doi.org/10.1016/j.aquatox.2006.05.010
  • Carmona, E. R., Benito, N., Plaza, T., & Recio-Sánchez, G. (2017). Green synthesis of silver nanoparticles by using leaf extracts from the endemic Buddleja globosa hope. Green Chemistry Letters and Reviews, 10(4), 250-256. https://doi.org/10.1080/17518253.2017.1360400
  • Connolly, M., Fernandez-Cruz, M. L., Quesada-Garcia, A., Alte, L., Segner, H., & Navas, J. M. (2015). Comparative cytotoxicity study of silver nanoparticles (AgNPs) in a variety of rainbow trout cell lines (RTL-W1, RTH-149, RTG-2) and primary hepatocytes. International journal of environmental research and public health, 12(5), 5386-5405. https://doi.org/10.3390/ijerph120505386
  • de Aragão, A. P., de Oliveira, T. M., Quelemes, P. V., Perfeito, M. L. G., Araújo, M. C., Santiago, J., ... & Quaresma, P. (2016). JR de Souza de Almeida Leite, DA da Silva, Green synthesis of silver nanoparticles using the seaweed gracilaria birdiae and their antibacterial activity. Arabian Journal of Chemistry. https://doi.org/10.1016/j.arabjc.2016.04.014
  • de Jesús Ruíz-Baltazar, Á., Reyes-López, S. Y., de Lourdes Mondragón-Sánchez, M., Estevez, M., Hernández-Martinez, A. R., & Pérez, R. (2018). Biosynthesis of Ag nanoparticles using Cynara cardunculus leaf extract: evaluation of their antibacterial and electrochemical activity. Results in Physics, 11, 1142-1149. https://doi.org/10.1016/j.rinp.2018.11.032
  • Demir, V., Bucher, J., Kropf, C., Arenz, M., & Segner, H. (2020). Comparative study of cytotoxicity by platinum nanoparticles and ions in vitro systems based on fish cell lines. Toxicology in vitro, 66, 104859. https://doi.org/10.1016/j.tiv.2020.104859
  • Du, J., Tang, J., Xu, S., Ge, J., Dong, Y., Li, H., & Jin, M. (2018). A review on silver nanoparticles-induced ecotoxicity and the underlying toxicity mechanisms. Regulatory Toxicology and Pharmacology, 98, 231-239. https://doi.org/10.1016/j.yrtph.2018.08.003
  • Onur, D. U. R. A., Tülek, A., Sönmez, İ., Erdoğuş, F.D., Yeşilayer, A., & Kepenekci, İ. (2019). Lantana camara L.(Lamiales: Verbenaceae)'nın sulu ekstraktı kullanılarak hazırlanan gümüş nanopartikül (AgNPs) uygulamalarının Buğday gal nematodu [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)]’na etkileri. Bitki Koruma Bülteni, 59(2), 49-53. https://doi.org/10.16955/bitkorb.485072
  • Farkas, J., Christian, P., Urrea, J. A. G., Roos, N., Hassellöv, M., Tollefsen, K. E., & Thomas, K. V. (2010). Effects of silver and gold nanoparticles on rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquatic Toxicology, 96(1), 44-52. https://doi.org/10.1016/j.aquatox.2009.09.016
  • fırat BARAN, M. (2019). Prunus avium kiraz yaprağı özütü ile gümüş nanopartikül (AgNP) sentezi ve antimikrobiyal etkisinin incelenmesi. Dicle Üniversitesi Mühendislik Fakültesi Mühendislik Dergisi, 10(1), 221-227. https://doi.org/10.24012/dumf.487255
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There are 46 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Semra Çiçek 0000-0002-2927-2793

Publication Date October 15, 2022
Submission Date April 15, 2022
Acceptance Date August 30, 2022
Published in Issue Year 2022 Volume: 12 Issue: 4

Cite

APA Çiçek, S. (2022). Cytotoxicity of silver nanoparticles obtained from Eruca vesicaria on rainbow trout gonad cell line-2 (RTG-2). Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 12(4), 1093-1101. https://doi.org/10.17714/gumusfenbil.1104370