Aktive c kinaz 1 reseptörünün taşınabilir potansiyostatla tayinine yönelik yeni bir biyosensör tasarımı

Year 2025, Volume: 15 Issue: 3, 667 - 676, 15.09.2025

Münteha Nur Sonuç Karaboğa

https://doi.org/10.17714/gumusfenbil.1654313

Abstract

Kanser biyobelirteçleri arasında dikkat çekici bir protein olan aktive edilmiş C kinaz 1 (RACK-1) hassas ve seçici tayini için ekran baskılı karbon elektrotlar (SPCE'ler) kullanılarak çiftli kendi kendine düzenlenen tek tabaka (SAMs) taşınabilir bir immünosensör geliştirilmiştir. Biyosensörün tasarımında 4-aminotiyofenol (4-ATP) ve (3-Aminopropil)trietoksi silan ile konjuge edilmiş altın nanopartiküller (AuNP'ler) kullanılmıştır. bu nanomalzemelere bağlanan biyotanıma ajanı, 0,001 ng/mL'lik bir tespit sınırıyla ilişkili olarak 0,002–0,8 ng/mL aralığında hedef molekül RACK-1'i belirleyebilmektedir. Çalışmanın en önemli noktası, biyosensörün tayin aralığı, depolama ömrü, seçicilik ve tekrarlanabilirlik gibi analitik özelliklerinin taşınabilir bir potansiyostat ile izlenebilmesidir. Çift SAMs tabanlı geliştirilen ve farklı proteinlerin varlığında ve yapay serum ortamında RACK-1’in analizi için yüksek seçicilik gösteren mevcut biyosensör tasarımı, insan serumu ile yapılacak ileri çalışmalar için ümit vericidir.

Keywords

Altın nanopartikül , Biyosensör , Kanser biyobelirteçleri , Kendiliğinden oluşan tek tabakalar , RACK-1

A useful architecture based on layer by layer self-assembled monolayers for the determination of receptor for activated c kinase 1 with a portable potentiostat

Abstract

A layer by layer self-assembled monolayer-based (SAMs) portable immunosensor was developed using screen printed carbon electrodes (SPCEs) for the sensitive and selective determination of receptor for activated C kinase 1 (RACK-1), which has a remarkable protein among cancer biomarkers. Gold nanoparticles (AuNPs) conjugated with 4-aminothiophenol (4-ATP) and (3-Aminopropyl)triethoxysilane were used in the design of the biosensor. The biorecognition element generated by powerful materials can determine the target molecule RACK-1 in the range of 0.002–0.8 ng/mL associated with a limit of detection of 0.001 ng/mL. The highlight of the study is that the analytical characteristics of the biosensor, such as the determination range, storage life, selectivity, and reproducibility, can be monitored with a portable potentiostat. The current biosensor design, which is developed based on layer by layer SAMs and shows high selectivity in the presence of different proteins and in artificial serum, is promising for further studies with human serum.

Keywords

Biosensor , Cancer biomarkers , Gold nanoparticles , RACK-1 , Self assembled monolayers

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