Trabzon Fatih Devlet Hastanesinden İzole Edilen Klinik Acinetobacter baumannii ve Pseudomonas aeruginosa Suşlarında Antibiyotik Direnç Genlerinin Araştırılması

Yıl 2023, , 450 - 455, 18.06.2023

Esma Akyıldız , Ayşegül Saral , Tuba Köse , Fatih Şaban Beriş , Azer Özad Düzgün

https://doi.org/10.37989/gumussagbil.1180332

Öz

Bu çalışmanın amacı Acinetobacter baumannii ve Pseudomonas aeruginosa izolatlarının antibiyotik duyarlılık profilini araştırmak ve bu izolatlarda direnç genlerinin varlığını belirlemektir. Direnç genlerinin tespiti PCR yöntemi ile değerlendirildi. Acinetobacter baumannii izolatlarında imipenem ve meropenem direnci sırasıyla %93,2 ve %91 olduğu görüldü. P. aeruginosa izolatlarında imipenem direnç oranı %53,9 ve meropenem direnç oranı %38,5 olarak belirlendi. Pseudomonas aeruginosa izolatları arasında aranan genlerden bir izolatta sadece OXA-23 tespit edildi. Acinetobacter baumannii izolatlarından bir izolatta OXA-24, 40 izolatta OXA-23 saptandı. Bu çalışma, CRPA (Karbapenem dirençli Pseudomonas aeruginosa) ve CRAB (Karbapenem dirençli Acinetobacter baumannii) izolatlarında karbapenem direncinden OXA-23'ün sorumlu olduğunu göstermektedir.

Anahtar Kelimeler

Acinetobacter baumannii, Karbapenem, OXA-23, Pseudomonas aeruginoasa

Destekleyen Kurum

Gümüşhane Üniversitesi

Proje Numarası

18.F5119.03.02

Investigation of Antibiotic Resistance Genes in Clinical Acinetobacter baumannii and Pseudomonas.aeruginosa Strains Isolated From Trabzon Fatih State Hospital

Öz

The aim of the study was to investigate the antibiotic susceptibility profile of Acinetobacter baumannii, and Pseudomonas aeruginosa isolates and to determine the presence of resistance genes in these isolates.The determination of resistance genes was assessed using the PCR method. Imipenem and meropenem resistance in Acinetobacter baumannii isolates were 93.2% and 91%, respectively. The imipenem resistance rate was 53.9% and meropenem resistance rate was 38.5% in Pseudomonas aeruginosa isolates. Of the genes sought among Pseudomonas aeruginosa isolates, only OXA-23 was detected in one isolate. Among Acinetobacter baumannii isolates, OXA-24 was detected in one isolate and OXA-23 in 40 isolates. This study demonstrates that OXA-23 is responsible for carbapenem resistance in CRPA (carbapenem resistant Pseudomonas aeruginosa) and CRAB (carbapenem resistant Acinetobacter baumannii) isolates.

Anahtar Kelimeler

Acinetobacter baumannii, Carbapenem, OXA-23, Pseudomonas aeruginoasa

Proje Numarası

18.F5119.03.02

Kaynakça

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