Exploration of the Relationship Between Routine Biochemistry Parameters, Scavenger Receptors, and Apoptotic Proteins During the Treatment Process of Acute Myocardial Infarction

Yıl 2024, , 370 - 381, 27.03.2024

Sema Tahtalıoğlu , Abdulkadir Çakmak , Gökhan Keskin , Burak Yazgan

https://doi.org/10.37989/gumussagbil.1321191

Öz

In this study, we aimed to investigate how the stent treatment process affects scavenger receptors and apoptotic protein levels in patients with myocardial infarction clinical picture. In this context, patients without coronary occlusive lesion on coronary angiography were included in the control group, and patients coronary occlusive lesion on coronary angiography were included in the infarction group. Routine biochemistry and cardiac markers from serum samples were measured with a biochemistry autoanalyzer. Blood samples were collected from the control group and patients with myocardial infarction (day 0, day 3 and day 30) and PBMCs were isolated with ficoll solution. LOX-1, CD36, CD68 and CXCL16 gene expressions in PBMCs were determined via qPCR. In addition, BAX, BCL-2 and Caspase-3 protein levels in serum samples were measured by ELISA method. When the results were evaluated, no significant difference was found between the groups in the routine biochemistry values for triglyceride, cholesterol and LDL. HDL levels were lower in the MI group. Conversely, glucose levels were higher in the MI group. Besides, cardiac markers, including troponin, CK-MB and creatine kinase, showed a significant increase in the MI group. However, there was no significant change in the gene expressions of LOX-1, CD36, CD68, and CXCL16 in PBMCs both between the control group and the MI group and between time-dependent MI groups (day 0, day 3 and day 30). Similarly, no significant changes were observed in the protein expressions of BAX, BCL-2 and Caspase-3. The non-significant difference in the expressions of scavenger receptors may possibly be due to the similarity of the lipid profile observed between the groups.

Anahtar Kelimeler

Acute Myocardial Infarction, BAX, Caspase 3, CD36, LOX-1

Akut Miyokard İnfarktüsü Tedavi Sürecinde Rutin Biyokimya Parametreleri, Çöpçü Reseptörler ve Apoptotik Proteinler Arasındaki İlişkinin İncelenmesi

Öz

Bu çalışmada miyokard infarktüsü klinik tablosuna sahip hastalarda stent tedavi sürecinin çöpçü reseptörler ve apoptotik protein düzeylerine nasıl etki gösterdiğinin incelenmesi amaçlanmıştır. Bu amaçla koroner anjiyografide koroner tıkayıcı lezyonu olmayanlar kontrol grubuna ve koroner anjiyografide belirgin koroner tıkayıcı lezyonu ile birlikte miyokard infarktüsü klinik tablosu bulunan hastalar infarktüs grubuna alınmıştır. Serum örneklerinden rutin biyokimya ve kardiyak belirteçler biyokimya otoanalizörü ile ölçülmüştür. Kontrol grubu ve Miyokard infarktüsü geçiren hastalardan (0. gün, 3. gün ve 30. gün) kan örnekleri alınmış ve PBMC’ler ficoll solüsyonuyla izole edilmiştir. PBMC’lerde LOX-1, CD36, CD68 ve CXCL16 gen ekspresyonları qPCR yöntemi ile belirlenmiştir. Ayrıca serum örneklerindeki BAX, BCL-2 ve Kaspaz-3 protein seviyeleri ELISA yöntemi ile ölçülmüştür. Sonuçlar değerlendirildiğinde rutin biyokimya değerlerinden trigliserid, kolesterol ve LDL kolesterol değerlerinde gruplar arasında anlamlı bir fark bulunamamıştır. HDL kolesterol Mİ gruplarında daha düşük bulunmuştur. Glukoz ise Mİ grubunda daha yüksek bulunmuştur. Mİ grubunda kardiyak belirteçler olan troponin, CK-MB ve kreatin kinaz değerleri anlamlı bir artış göstermiştir. Ancak hem kontrol grubu ile Mİ grubu arasında hem de Mİ grubunda zamana bağlı (0. gün, 3. gün ve 30. gün) PBMC’lerin LOX-1, CD36, CD68 ve CXCL16 gen ekspresyonlarında anlamlı bir değişim bulunamamıştır. Benzer olarak serum BAX, BCL-2 ve Kaspaz-3 protein ekspresyonlarında da önemli bir değişiklik gözlenmemiştir. Çöpçü reseptör ekspresyonlarında anlamlı bir artışın olmaması muhtemelen gruplar arasındaki benzer lipid profilinin gözlenmesinden kaynaklanabilir.

Anahtar Kelimeler

Akut Miyokard İnfarktüsü, BAX, CD36, Kaspaz 3, LOX-1

Kaynakça

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