Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi

Year 2019, Volume: 8 Issue: 4, 438 - 443, 31.12.2019

Afşin Ahmet Kaya Murat Şentürk Elif Çelenk Kaya

Abstract

Glutatyon redüktaz (Glutatyon: NADP⁺
oksidoredüktaz, EC 1.8.1.7; GR) düşük (GSH) veya yüksek molekül ağırlıklı
(GSSG) disülfit substratları ile indirgenmiş piridin nükleotidleri (NADPH)
arasında elekton transferini katalizler. GR inhibitörleri son zamanlarda
anti-sıtma ve kansere karşı faaliyetlerinin keşfedilmesi nedeniyle oldukça
popüler hale gelmiştir.

Enzimatik aktivite Beutler metodu
kullanılarak Spektrofotometre kullanılarak 25°C’de yapıldı. Ölçüm sistemi 100
mM Tris-HCl tamponunda (pH 8.0), 0,5 mM EDTA, 3,3 mM GSSG ve 0,1 mM NADPH içeriyordu.
Nitrobenzen (1), 3,5-Dinitrosalisilik
asit (2), (2,4-Dinitrofenil) ((4-(prop-1-en-2-yl)
sikloheks-1-enil)-metil) sulfan (3),
((7,7-dimetilbisiklo [2.2.1] heptan-1-il)metil)-(2,4-dinitrofenil)sulfan (4) ve ((2-kloro-7,7-dimetilbisiklo [2.2.1]
heptan-1-il)metil)-(2,4-dinitrofenil)sulfan (5) maddelerinin GR enzimi üzerindeki in vitro inhibisyon etkisi
incelendi.

İnhibisyon etkisi gösteren maddeler için %
aktivite-[I] grafikleri çizilerek IC₅₀ değerleri bulunmuştur. Bu
maddelerin GR için IC₅₀ değerlerinin mikromolar seviyesinde olduğu
gözlemlendi.

Keywords

Nitrolu madde, Glutatyon redüktaz

Supporting Institution

Ağrı İbrahim Çeçen Üniversitesi Bap

Project Number

ECZF.19.001

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