Trabzon Fatih Devlet Hastanesinden İzole Edilen Klinik Acinetobacter baumannii ve Pseudomonas aeruginosa Suşlarında Antibiyotik Direnç Genlerinin Araştırılması

Year 2023, Volume: 12 Issue: 2, 450 - 455, 18.06.2023

Esma Akyıldız , Ayşegül Saral , Tuba Köse , Fatih Şaban Beriş , Azer Özad Düzgün

https://doi.org/10.37989/gumussagbil.1180332

Abstract

Bu çalışmanın amacı Acinetobacter baumannii ve Pseudomonas aeruginosa izolatlarının antibiyotik duyarlılık profilini araştırmak ve bu izolatlarda direnç genlerinin varlığını belirlemektir. Direnç genlerinin tespiti PCR yöntemi ile değerlendirildi. Acinetobacter baumannii izolatlarında imipenem ve meropenem direnci sırasıyla %93,2 ve %91 olduğu görüldü. P. aeruginosa izolatlarında imipenem direnç oranı %53,9 ve meropenem direnç oranı %38,5 olarak belirlendi. Pseudomonas aeruginosa izolatları arasında aranan genlerden bir izolatta sadece OXA-23 tespit edildi. Acinetobacter baumannii izolatlarından bir izolatta OXA-24, 40 izolatta OXA-23 saptandı. Bu çalışma, CRPA (Karbapenem dirençli Pseudomonas aeruginosa) ve CRAB (Karbapenem dirençli Acinetobacter baumannii) izolatlarında karbapenem direncinden OXA-23'ün sorumlu olduğunu göstermektedir.

Keywords

Acinetobacter baumannii, Karbapenem, OXA-23, Pseudomonas aeruginoasa

Supporting Institution

Gümüşhane Üniversitesi

Project Number

18.F5119.03.02

Investigation of Antibiotic Resistance Genes in Clinical Acinetobacter baumannii and Pseudomonas.aeruginosa Strains Isolated From Trabzon Fatih State Hospital

Abstract

The aim of the study was to investigate the antibiotic susceptibility profile of Acinetobacter baumannii, and Pseudomonas aeruginosa isolates and to determine the presence of resistance genes in these isolates.The determination of resistance genes was assessed using the PCR method. Imipenem and meropenem resistance in Acinetobacter baumannii isolates were 93.2% and 91%, respectively. The imipenem resistance rate was 53.9% and meropenem resistance rate was 38.5% in Pseudomonas aeruginosa isolates. Of the genes sought among Pseudomonas aeruginosa isolates, only OXA-23 was detected in one isolate. Among Acinetobacter baumannii isolates, OXA-24 was detected in one isolate and OXA-23 in 40 isolates. This study demonstrates that OXA-23 is responsible for carbapenem resistance in CRPA (carbapenem resistant Pseudomonas aeruginosa) and CRAB (carbapenem resistant Acinetobacter baumannii) isolates.

Keywords

Acinetobacter baumannii, Carbapenem, OXA-23, Pseudomonas aeruginoasa

Project Number

18.F5119.03.02

References

• 1. Wang, X. and Qin, L.J. (2019). “A review on Acinetobacter baumannii”. Journal of Acute Disease, 8 (1), 16-20. https://doi.org/10.4103/2221-6189.250373
• 2. Özsoy, M, Sönmezer, M. and Kınıklı, S. (2019). “A recent problem at hospitals: treatment of multi-drug resistant Acinetobacter baumanii infections”. Anadolu Güncel Tıp Dergisi, 1 (2),37-41. https://doi.org/10.38053/agtd.526530
• 3. Lee, C.R, Lee, J.H, Park, M, Park, K.S, Bae, I.K, Kim, Y.B, Cha, C.J, Jeong, B.C. and Lee, S.h. (2017). “Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options”. Frontiers in Cellular and Infection Microbiolology,13 (7), 55.https://doi.org 10.3389/fcimb.2017.00055
• 4. Demirci, M, Yigin, A, Demir, C. (2019). “Karbapeneme Direncli Acinetobacter baumannii Suşlarinda OXA Tipi Karbapenemaz Genlerinin Dagiliminin Gercek Zamanli Polimeraz Zincir Reaksiyonu Yontemiyle Incelenmesi”. KLIMIK Journal, 32, 123-126. https://doi.org/10.5152/kd.2019.29
• 5. Davoodi, S, Boroumand, M.A, Sepehriseresht, S. and Pourgholi, L. (2015). “Detection of VIM-and IMP-type metallo-beta-lactamase genes in Acinetobacter baumannii isolates from patients in two hospitals in Tehran”. Iranian Jounal of Biotechnology, 13(1), 63-67. https://doi.org/10.15171/ijb1088
• 6. Girija, S.A, Jayaseelan, V.P and Arumugam, P. (2018). “Prevalence of VIM-and GIM-producing Acinetobacter baumannii from patients with severe urinary tract infection”. Acta Microbiologica et Immunologica Hungarica, 65(4), 539-550. https://doi.org/10.1556/030.65.2018.038
• 7. Kal Çakmaklıoğulları, E. and Kuru, C. (2019). “Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains: Evaluation in Different Sample Types”.ANKEM Journal, 33 (2), 37-42. https://doi.org/ 10.5222/ankem.2019.197
• 8. Rocha, A.J, Barsottini, M.R.D.O, Rocha. R.R, Laurindo, V.M, Laurindo de Moraes, F.L and Rocha, S.L. (2019). “Pseudomonas aeruginosa: Virulence Factors and Antibiotic Resistance Genes”. Brazilian Archives of Biology and Technology, 62, e19180503. https://doi.org/ 10.1590/1678-4324-2019180503
• 9. Strateva, T. and Yordanov, D. (2009). “Pseudomonas aeruginosa–a phenomenon of bacterial resistance”. Jounal of Medical Microbiology, 58(9), 1133-1148. https://doi.org/10.1099/jmm.0.009142-0.
• 10. Petrova, A.P, Stanimirova, I.D, Ivanov. I.N, Petrov, M.M, Miteva-Katrandzhieva, T.M, Grivnev, V.I, Kardjeva, V.S, Kantardzhiev, T.V. and Murdjeva, M.A. (2017). “Carbapenemase production of clinical isolates Acinetobacter baumannii and Pseudomonas aeruginosa from a Bulgarian University hospital”. Folia Medica (Plovdiv), 59 (4), 413-422. https://doi.org/10.1515/folmed-2017-0060
• 11. EUCAST. (2017). European Committee on Antimicrobial Susceptibility Testing, Breakpoint tables for interpretation of MICs and zone diameters. In. European Society of Clinical Microbiology and Infectious Diseases Basel. 12. Woodford, N, Ellington, M.J, Coelho, J.M, Turton, J.F, Ward, M.E, Brown, S, Amyes, S.G.B. and Livermore, D.M. (2006). “Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.”International Journal of Antimicrobial Agents, 27 (4), 351-353. https://doi.org/ 10.1016/j.ijantimicag.2006.01.004
• 13. Iraz, M, Özad Düzgün, A, Sandallı, C, Doymaz, M.Z, Akkoyunlu, Y, Saral, A, Peleg, A.Y, Özgümüş, O.B, Beriş, F.Ş, Karaoğlu, H. and Çopur Çiçek, A. (2015). “Distribution of β-lactamase genes among carbapenem-resistant Klebsiella pneumoniae strains isolated from patients in Turkey”. Annals of Laboratory Medicine, 35 (6), 595-601. https://doi.org/ 10.3343/alm.2015.35.6.595
• 14. Ellington, M.J, Kistler, J, Livermore, D.M. and Woodford, N. (2007). “Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases”. Journal of Antimicrobial Chemotherapy, 59 (2), 321-322. https://doi.org/ 10.1093/jac/dkl481
• 15. El-Badawy, M.F, Tawakol, W.M, El-Far, S.W, Maghrabi, I.A, Al-Ghamdi, S.A, Mansy, M.S, Ashour, M.S. and Shohayeb, M.M. (2017). “Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients”. International Journal of Microbiology, 2017, 8050432. https://doi.org/ 10.1155/2017/8050432
• 16. Isler, B, Doi, Y, Bonomo, R.A. and Paterson, D.L. (2019). “New treatment options against carbapenem-resistant Acinetobacter baumannii infections”. Antimicrobial Agents and Chemotherapy, 63 (1), e01110-18. https://doi.org/ 10.1128/AAC.01110-18
• 17. Ramadan, R.A, Gebriel, M.G, Kadry, H.M. and Mosallem, A. (2018). “Carbapenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa: characterization of carbapenemase genes and E-test evaluation of colistin-based combinations”. Infection and Drug Resistance, 11, 1261-1269. https://doi.org/ 10.2147/IDR.S170233
• 18. Kazi, M, Nikam, C, Shetty, A, Rodrigues, C. (2015). Dual-tubed multiplexPCR for molecular characterization of carbapenemases isolated among Acinetobacter spp. and Pseudomonas spp. J Appl Microbiol 118 (5), 1096–1102. https://doi.org/10.1111/jam.12770.
• 19. Hsu, L-Y, Apisarnthanarak, A, Khan, E, Suwantarat, N, Ghafur, A. and Tambyah, P.A. (2017). “Carbapenem-resistant Acinetobacter baumannii and Enterobacteriaceae in south and southeast Asia”. Clinical Microbiology Reviews, 30 (1), 1-22. https://doi.org/ 10.1128/CMR.masthead.30-1
• 20. Güven, T, Yılmaz, G, Güner, H.R, Kalem, A.K, Eser, F. and Taşyaran, M.A. (2014). “Increasing resistance of nosocomial Acinetobacter baumannii: are we going to be defeated?” Turkish Journal of Medical Science, 44(1), 73-8. https://doi.org/ 10.3906/sag-1211-21
• 21. Boral, B, Unaldi, Ö, Ergin, A, Durmaz, R, Eser, Ö.K. and Acinetobacter Study Group. (2019). “A prospective multicenter study on the evaluation of antimicrobial resistance and molecular epidemiology of multidrug-resistant Acinetobacter baumannii infections in intensive care units with clinical and environmental features”. Annals of Clinical Microbiology and Antimicrobials, 18 (1), 19. https://doi.org/ 10.1186/s12941-019-0319-8
• 22. Nitz, F, de Melo, B.O, da Silva, L,C,N, de Souza Monteiro, A, Marques, S.G, Monteiro-Neto, V, de Jesus Gomes Turri, R, Junior, A.D.S, Conceição, P.C.R, Magalhães, H.J.C, Zagmignan, A, Ferro, T.A.F, Bomfim, M.R.Q. (2021). “Molecular Detection of Drug-Resistance Genes of blaOXA-23-blaOXA-51 and mcr-1 in Clinical Isolates of Pseudomonas aeruginosa”. Microorganisms, 9 (4), 786. doi: 10.3390/microorganisms9040786.
• 23. Xiao, S.Z, Chu, H.Q, Han, L.Z, Zhang, Z.M, Li, B, Zhao, L, Xu, L. (2016). “Resistant mechanisms and molecular epidemiology of imipenem-resistant Acinetobacter baumannii”. Molecular Medıcıne Reports, 14 (3), 2483-8. doi: 10.3892/mmr.2016.5538.
• 24. Castilho, S.R.A, Godoy, C.S.M, Guilarde, A.O, Cardoso, J.L, Andre, M.C.P, Junqueira-Kipnis, A.P, Kipnis, A. (2017). “Acinetobacter baumannii strains isolated from patients in intensive care units in Goiania, Brazil: Molecular and drug susceptibility profiles”. Plos One, 12 (5), e0176790. doi: 10.1371/journal.pone.0176790.