Kuersetin'in Sipermetrin Kaynaklı Mide Hasarı Üzerine Etkileri: Oksidatif Stres, Enflamasyon ve Apoptozun Rolü

Year 2023, Volume: 12 Issue: 2, 556 - 566, 18.06.2023

Nurhan Akaras , Cihan Gür , Hasan Şimşek , Sibel Çiğdem Tuncer

https://doi.org/10.37989/gumussagbil.1225539

Cited By: 1

Abstract

Bu çalışma, ratlarda sipermetrin (CYP) kaynaklı gastrotoksisite üzerine kuersetin'in (QUE) etkilerini araştırmak için yapıldı. 35 adet Sprague Dawley ratı, her grupta 7 tane olacak şekilde rastgele beş gruba ayrıldı. Çalışmada, ratlara 28 gün boyunca 25 mg/kg sipermetrin uygulandıktan 30 dakika sonra oral olarak 25 ve 50 mg/kg QUE verildi. Mide dokularında oksidatif stres, inflamasyon, ER stres, apoptoz ve otofaji belirteçleri biyokimyasal olarak analiz edildi. Ayrıca mide dokusu mikroskobik değerlendirme için histolojik analiz yapıldı. Sonuçlar, QUE'nin CYP kaynaklı lipit peroksidasyonunu (MDA) düşürerek ve GSH, SOD, KAT ve GPx aktivitelerini artırarak doku hasarını önlediğini ortaya koydu. Ayrıca NF-𝜅B, IL-1β, TNF-α, iNOS ve COX-2 gibi inflamatuar belirteçleri baskılayarak anti-inflamatuar etki gösterdi. CYP kaynaklı artan PERK, ATF6, Kaspaz-3 ve Beklin-1 belirteçlerini QUE uygulanması aşağı regüle etti. Ek olarak, CYP’ye bağlı olarak oluşan mide dokusundaki patolojik doku hasarını QUE verilmesi iyileştirdi. Bu çalışmanın verileri Que nin oksidatif stresi, enflamasyonu, ER stresi, apoptozu ve otofajiyi iyileştirerek CYP kaynaklı mide toksisitesini baskıladığını göstermektedir.

Keywords

Gastrotoksisite, Kuersetin, Oksidatif Stres, Rat, Sipermetrin

Supporting Institution

Destekleyen bir kurum yoktur.

Effects of Quercetin on Cypermethrin-Induced Stomach Injury: The Role of Oxidative Stress, Inflammation, and Apoptosis.

Abstract

This study was conducted to investigate the effects of quercetin (QUE) on cypermethrin (CYP) induced gastrotoxicity in rats. 35 Sprague-Dawley rats were randomly divided into five groups, 7 in each group. In the study, 25 and 50 mg/kg QUE were administered orally 30 min after 25 mg/kg cypermethrin was administered to rats for 28 days. Oxidative stress, inflammation, ER stress, apoptosis and autophagy markers were biochemically analyzed in gastric tissues. Additionally, histological analysis was performed for microscopic evaluation of gastric tissue. The results revealed that QUE prevented tissue damage by reducing CYP-induced lipid peroxidation (MDA) and increasing GSH, SOD, CAT and GPx activities. It also showed anti-inflammatory effect by suppressing inflammatory markers such as NF-𝜅B, IL-1β, TNF-α, iNOS and COX-2. QUE administration down-regulated CYP-induced increased PERK, ATF6, Caspase-3 and Beclin-1 markers. In addition, administration of QUE ameliorated the pathological tissue damage in gastric tissue due to CYP. The data of this study show that Que suppresses CYP-induced gastric toxicity by reducing oxidative stress, inflammation, ER stress, apoptosis a autophagy.

Keywords

Cypermethrin, Gastrotoxicity, Oxidative Stress, Quercetin, Rat

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