PI3K AND MTOR IMMUNOREACTIVITY IN TESTICULAR TISSUE IN EXPERIMENTAL ALCOHOL ADDICTION MODEL

Year 2024, Volume: 13 Issue: 3, 1119 - 1124, 26.09.2024

Osman Öztürk , Aslı Okan Oflamaz , Mustafa Kurt , Ece Eroğlu , Seher Yilmaz , A. Cihangir Uğuz , Mert Ocak , Züleyha Doğanyiğit

https://doi.org/10.37989/gumussagbil.1412538

Abstract

Alcohol use disorder has negative effects on the reproductive system through the development of oxidative stress and its genotoxic effects on DNA integrity. Acute and chronic alcohol use adversely affects the male reproductive system. Phosphatidylinositol-3-kinase (PI3K) and mammalian target of rapamycin (mTOR) levels, which are involved in oxidative stress, may be important in the reproductive system in alcohol use disorder.
PI3K and mTOR immunoreactivities were evaluated in testicular tissue in an experimental acute and chronic alcohol intake model in male rats. Rats were divided into 3 groups as control male (n=7), acute male (n=7) and chronic male model (n=7). Histopathological analysis of testicular tissues taken from the experimental groups was performed by hematoxylin-eosin (H&E) staining. Then, testicular tissues of the experimental groups were dissected and PI3K and mTOR expressions were determined by immunohistochemistry method.
According to the H&E staining results, when the experimental groups were compared with the control group; It was observed that spermatozoa were less or absent in acute and chronic groups. mTOR and PI3K expressions were significantly increased in testicular tissues belonging to chronic and acute alcohol model groups. In the chronic alcohol model, both mTOR and PI3K expressions were significantly increased compared to the acute and control groups.
Our research reveal that PI3K and mTOR molecules, which are involved in oxidative stress in acute and chronic alcohol intake, may be associated with damage to the reproductive system. PI3K and mTOR proteins, can be targeted at the point of treatment against alcohol-induced reproductive damage.

Keywords

Oxidative stress, Alcohol addiction, Reproductive system, Immunohistochemistry

DENEYSEL ALKOL BAĞIMLILIĞI MODELİNDE TESTİS DOKUSUNDA PI3K VE MTOR İMMÜNOREAKTİVİTESİ

Abstract

Alkol kullanım bozukluğu, oksidatif stresin gelişmesi ve bunun DNA bütünlüğü üzerindeki genotoksik etkileri yoluyla üreme sistemi üzerinde olumsuz etkilere sahiptir. Akut ve kronik alkol kullanımı erkek üreme sistemini olumsuz etkiler. Oksidatif streste rol oynayan fosfatidilinositol-3-kinaz (PI3K) ve rapamisin memeli hedefi (mTOR) düzeyleri, alkol kullanım bozukluğunda üreme sistemi açısından önemli olabilir.
PI3K ve mTOR immünoreaktiviteleri, erkek sıçanlarda deneysel bir akut ve kronik alkol alımı modelinde testis dokusunda değerlendirildi. Sıçanlar kontrol erkek (n=7), akut erkek (n=7) ve kronik erkek model (n=7) olmak üzere 3 gruba ayrıldı. Deney gruplarından alınan testis dokularının histopatolojik analizi hematoksilen-eozin (H&E) boyama ile yapıldı. Daha sonra deney gruplarının testis dokuları diseke edilerek immünohistokimya yöntemiyle PI3K ve mTOR ekspresyonları belirlendi.
H&E boyama sonuçlarına göre deney grupları kontrol grubu ile karşılaştırıldığında; Akut ve kronik gruplarda spermatozoanın az olduğu veya hiç olmadığı görüldü. Kronik ve akut alkol model gruplarına ait testis dokularında mTOR ve PI3K ekspresyonları anlamlı derecede arttı. Kronik alkol modelinde hem mTOR hem de PI3K ekspresyonları akut ve kontrol gruplarına göre anlamlı derecede arttı.
Araştırmamız, akut ve kronik alkol alımında oksidatif strese karışan PI3K ve mTOR moleküllerinin üreme sistemindeki hasarla ilişkili olabileceğini ortaya koymaktadır. PI3K ve mTOR proteinleri, alkole bağlı üreme hasarına karşı tedavi noktasında hedeflenebilir.

Keywords

Oksidatif stres, Alkol bağımlılığı, Üreme sistemi, İmmünohistokimya

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