Sclerotinia minor Jagger üzerine potansiyel biyokontrol ajanlarının değerlendirilmesi

Year 2022, , 480 - 490, 26.12.2022

Raziye Koçak , Özden Salman , Nuh Boyraz

https://doi.org/10.29050/harranziraat.1198298

Abstract

Bu çalışma farklı bitkilerin rizosferik toprak bölgesinden izole edilen 38 bakteri izolatının ayçiçeğinde beyaz çürüklük etmenlerinden biri olan Sclerotinia minor Jagger’e karşı antagonistik etkilerini belirlemek amacıyla yapılmıştır. In vitro koşullarda test edilen bakterilerin yaklaşık %37’si orta ya da yüksek düzeyde patojenin misel gelişimini baskılamıştır. In vitro koşullarda patojeni yüksek düzeyde (%80-100) engelleyen bakteriler (Pseudomonas chlororaphis IDV5, Bacillus amyloliquefaciens IEB1, Bacillus cereus IPT3, Stenotrophomonas sp. IGL1, henüz teşhis edilememiş 2 bakteri izolatı (IFG1 ve IFG2)) daha sonra iklim odası koşullarında saksı çalışmaları ile test edilmiştir. Araştırma sonuçlarımıza göre saksı denemelerinde Pseudomonas chlororaphis dışında tüm bakteriler %100 etkili olmuştur. Ayrıca in vitro’da S. minor’e karşı etkisiz olmasına rağmen, daha önce yaptığımız farklı çalışmalarda in vivo koşullarda başarılı olduğunu tespit ettiğimiz Pseudomonas koreensis (IFG4)’de denemeye dahil edilmiş ve in vivo’da etkili bulunmuştur. Bu sonuç bazı rizosferik bakterilerin patojenin baskılanmasında rol oynayan bazı engelleyici özelliklerinin bitki ile bir araya geldiğinde ortaya çıktığını göstermektedir. Sonuç olarak çalışmamız biyolojik mücadelenin ayçiçeğinde toprak patojenlerini kontrol etmenin alternatiflerinden biri olduğunu ve bu antagonistik bakterilerin diğer özellikleri yönünden de araştırıldıktan sonra biyoajan olarak kullanılabileceğini ortaya koymaktadır.

Keywords

Sclerotinia minor, Antagonistik etki, Biyokontrol, Pseudomonas sp., Bacillus sp.

Evaluation of potential biocontrol agents on Sclerotinia minor Jagger

Abstract

This study was carried out to determine the antagonistic effects of 38 bacterial isolates isolated from the rhizospheric soil region of different plants against Sclerotinia minor Jagger, which is one of the causative agents of white rot in sunflower. Approximately 37% of the bacteria tested in vitro suppressed mycelial growth of the pathogen at moderate or high levels. Bacteria that inhibit pathogens at a high level (80-100%) in vitro (Pseudomonas chlororaphis IDV5, Bacillus amyloliquefaciens IEB1, Bacillus cereus IPT3, Stenotrophomonas sp. IGL1, 2 unidentified bacterial isolates (IFG1 and IFG2)) were potted under climatic chamber conditions tested by work. According to our research results, all bacteria except Pseudomonas chlororaphis were 100% effective in pot experiments. In addition, although it is ineffective against S. minor in vitro, Pseudomonas koreensis (IFG4), which we have found to be successful in vivo conditions in different studies we have done before, was included in the trial and was found effective in this study as well. This result shows that some inhibitory properties of some rhizospheric bacteria, which play a role in suppressing the pathogen, appear when they come together with the plant. In conclusion, our study reveals that biological control is one of the alternatives to control soil pathogens in sunflower and can be used as a bioagent after investigating other properties of these antagonistic bacteria.

Keywords

Sclerotinia minor, Antagonist effect, Biocontrol, Psudomonas sp., Bacillus sp.

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