New approaches to the control of Neodiprion sertifer (Geoffroy, 1785) (Hymenoptera: Diprionidae)

Year 2024, , 192 - 200, 21.06.2024

Hilal Tunca , Damla Çayci

https://doi.org/10.29050/harranziraat.1407990

Abstract

Neodiprion sertifer (Geoffroy) (Hymenoptera: Diprionidae) is a major pest on Pinus spp. in Europe. In this study, new control methods were tried to control N. sertifer. The effectiveness of Bauveria bassiana (Nostalgist, Bb %1.5 1 x 10 8 kob /ml min, 250 ml/100L), azadirachtin (Nimbecidine, 0.3g azadirachtin/l, 500 ml/da-100L; Nimiks4.5, 40g azadirachtin /l, 200 ml/100L; Neem Azal ®-T/S, 10 g azadirachtin /l, 300ml/100L) and spinosad (Oread, 480 g spinosad/l, 10ml/100L) was tested on N. sertifer larvae. In addition, the effect of the idiobiont parasitoid Bracon hebetor (Say) (Hymenoptera: Braconidae) venom was evaluated. Bauveria bassiana, spinosad and azadirachtin were applied to the larvae by spraying method. Different numbers of B. hebetor females (1-5 females) were also tested on N. sertifer larvae. All experiments were carried out at 25 ± 1°C, 60-70% relative humidity and 16L:8D h photoperiod. After B. bassiana application, the survival rates were 71.66% and 61.66% at the 6th day and 7th day. The maximum efficacy was obtained with azadirachtin in Nimiks 4.5 (40 g azadirachtin/l). The highest mortality (100.0%) was found at 24 h after the application of spinosad (10ml/100l). The most effective combination of B. hebetor female venom on N. sertifer larvae is 5 females and 7 days duration. The study shows that spinosad, azadirachtin and B. hebetor female venom can achieve effective control of N. sertifer larvae.

Keywords

Neodiprion sertifer, Bauveria bassiana, spinosad, azadirahtin, Bracon hebetor venom

Neodiprion sertifer (Geoffroy, 1785) (Hymenoptera: Diprionidae)’in mücadelesinde yeni yaklaşımlar

Abstract

Neodiprion sertifer (Geoffroy) (Hymenoptera: Diprionidae) Avrupa’da Pinus spp.‘nin en önemli zararlısı konumundadır. Bu çalışmada N. sertifer’in mücadelesi için yeni mücadele yöntemleri denenmiştir. Bauveria bassiana (Nostalgist, Bb %1.5 1 x 10 8 kob /ml min, 250 ml/100L), azadirachtin (Nimbecidine, 0.3g azadirachtin, 500 ml/da-100L, Nimiks4.5, 40g azadirachtin /l, 200 ml/100L; Neem Azal ®-T/S, 10 g azadirachtin /l,300ml/100L) ve spinosad’ın (Oread, 480 g spinosad/l, 10ml/100L) etkinliği N. sertifer larvaları üzerinde denenmiştir. Ayrıca idiobiont parazitoit Bracon hebetor (Say) (Hymenoptera: Braconidae)‘un venomu da değerlendirilmiştir. Bauveria bassiana, spinosad ve azadirachtin larvalara püskürtme yoluyla uygulanmıştır. Farklı sayıdaki B. hebetor dişisi de (1-5 dişi) N. sertifer üzerinde test edilmiştir. Bütün denemeler 25 ± 1°C, 60-70% orantılı nem ve 16:8 saat aydınlık:karanlık koşullarda gerçekleştirilmiştir. Bauveria bassiana uygulamasından sonra larvaların yaşam oranı 6. ve 7. günde %71.66 ve %61.66 olarak bulunmuştur. Azadirachtinde maksimum etkinlik Nimiks 4.5 (40 g azadirachtin/l)‘de elde edilmiştir. En yüksek ölüm oranı (%100) spinosad (10ml/100l) uygulamasından 24 saat sonra bulunmuştur. B. hebetor dişi venomunun N. sertifer larvaları üzerinde en etkili olduğu kombinasyon 5 dişi ve 7 gün süredir. Bu çalışma spinosad, azadirachtin ve B. hebetor dişi venomunun N. sertifer larvaları üzerinde etkili bir kontrol sağlayabileceğini göstermiştir.

Keywords

Neodiprion sertifer, Bauveria bassiana, spinosad, azadirachtin, Bracon hebetor venom

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