Adıyaman İlinde Tüketime Sunulan Tavuk Etlerinde Salmonella Spp. Yaygınlığının Son Zamanlarda Geliştirilen Biotinil-Tiramid Yöntemi İle Belirlenmesi

Abstract

Dünya genelinde, Salmonella spp. nörovirüslerden sonra en fazla besin zehirlenmesine sebebiyet veren patojenik bakterilerdir. Salmonella cinsine ait 2600’den fazla serovar tanımlanmış olup, bu cinse ait serovarların en çok tercih ettiği konak kanatlı hayvanlardır. Dolayısıyla, Salmonella cinsine ait serovarlarının sebebiyet verdiği gıda zehirlenmelerinin en yaygın olanı kümes hayvanları etlerinin (özellikle tavuk eti ve bu etin kullanıldığı mamullerin) tüketilmesiyle ortaya çıkmaktadır. Bu çalışmada Adıyaman İli’nde tüketime sunulan kümes hayvanı (tavuk) etlerinde Salmonella cinsine ait bakterilerin yaygınlığının belirlenmesi amaçlanmıştır. Bu amaç için Salmonella spp.’ye spesifik olarak bağlanabilen manyetik mikro boncuklar kullanılmış ve immünomanyetik separasyon (IMS) yapılarak bakteriler izole edilmiştir. Daha sonra tiramid amplifikasyonu (TA) yardımı ile bu patojenik bakterilerin kanatlı etlerindeki bulunma oranları belirlenmiştir. Bu çalışmada perakende olarak satılan tavuk etlerinden rastgele olarak seçilen 124 örnek çalışılmış ve bunların 35 (% 28.23) adedinde Salmonella spp.’ye rastlanılmıştır.

Keywords

• Salmonella,Biyotin,Kanatlı etleri,Manyetik boncuklar,Sinyal amplifikasyonu

Determinaton of Salmonella spp. Prevalence by a Recently Developed Biotinyl-Tyramide Signal Amplification from Retailed Poultry Meat in Adıyaman Province

Abstract

Worldwide, species belonging to the genus Salmonella are pathogenic bacteria and are the second most food poisoning causative agents after noroviruses. More than 2600 serovar of Salmonella have been defined, and their primary reservoir is poultry. Therefore, salmonellae serovars are most frequently associated with consumption of poultry meat and poultry products, which leads to Salmonella-related foodborne illnesses. The main purpose of this study was to determine the prevalence of Salmonella spp. by use of biotinyl-tyramide signal amplification from retail poultry (chicken) meat in Adıyaman province. For this purpose, magnetic micro-beads which can specifically bind to Salmonella spp., was used for isolation by applying Immunomagnetic Separation (IMS), and Tyramide Amplification (TA) was used for detection of the bacteria in order to determine the presence (by ratio) of Salmonella spp. in poultry meat. In this study, 124 samples of poultry meat were studied and 35 (28.23%) of them were Salmonella positive.

Keywords

• Salmonella,Biotin,Poultry,Magnetic beads,Signal amplification

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